Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01680 and BWI76_RS01685 are separated by 196 nucleotides BWI76_RS01685 and BWI76_RS01690 are separated by 185 nucleotides
BWI76_RS01680: BWI76_RS01680 - transcriptional regulator IclR, at 342,127 to 342,954
_RS01680
BWI76_RS01685: BWI76_RS01685 - methionine synthase, at 343,151 to 346,834
_RS01685
BWI76_RS01690: BWI76_RS01690 - membrane protein, at 347,020 to 348,651
_RS01690
Position (kb) 343
344
345
346
347 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 342.297 kb on - strand, within BWI76_RS01680 at 342.302 kb on - strand, within BWI76_RS01680 at 342.323 kb on - strand, within BWI76_RS01680 at 342.379 kb on - strand, within BWI76_RS01680 at 342.413 kb on - strand, within BWI76_RS01680 at 342.525 kb on + strand, within BWI76_RS01680 at 342.533 kb on - strand, within BWI76_RS01680 at 342.533 kb on - strand, within BWI76_RS01680 at 342.773 kb on + strand, within BWI76_RS01680 at 343.064 kb on - strand at 343.148 kb on + strand at 343.148 kb on + strand at 343.148 kb on + strand at 343.332 kb on - strand at 343.406 kb on - strand at 343.456 kb on + strand at 343.461 kb on + strand at 343.498 kb on + strand at 343.606 kb on + strand, within BWI76_RS01685 at 343.627 kb on + strand, within BWI76_RS01685 at 343.646 kb on - strand, within BWI76_RS01685 at 343.880 kb on + strand, within BWI76_RS01685 at 343.930 kb on - strand, within BWI76_RS01685 at 344.059 kb on + strand, within BWI76_RS01685 at 344.495 kb on - strand, within BWI76_RS01685 at 344.500 kb on - strand, within BWI76_RS01685 at 344.505 kb on - strand, within BWI76_RS01685 at 344.523 kb on + strand, within BWI76_RS01685 at 344.691 kb on - strand, within BWI76_RS01685 at 344.742 kb on + strand, within BWI76_RS01685 at 344.742 kb on + strand, within BWI76_RS01685 at 344.742 kb on + strand, within BWI76_RS01685 at 344.902 kb on - strand, within BWI76_RS01685 at 345.344 kb on + strand, within BWI76_RS01685 at 345.350 kb on - strand, within BWI76_RS01685 at 345.350 kb on - strand, within BWI76_RS01685 at 345.702 kb on - strand, within BWI76_RS01685 at 345.702 kb on - strand, within BWI76_RS01685 at 345.702 kb on - strand, within BWI76_RS01685 at 345.713 kb on - strand, within BWI76_RS01685 at 345.938 kb on + strand, within BWI76_RS01685 at 345.971 kb on - strand at 346.016 kb on - strand, within BWI76_RS01685 at 346.086 kb on - strand, within BWI76_RS01685 at 346.116 kb on - strand, within BWI76_RS01685 at 346.329 kb on + strand, within BWI76_RS01685 at 346.413 kb on - strand, within BWI76_RS01685 at 346.575 kb on + strand at 346.610 kb on - strand at 346.756 kb on - strand at 346.872 kb on + strand at 346.909 kb on + strand at 346.909 kb on + strand at 346.910 kb on - strand at 346.983 kb on - strand at 347.029 kb on + strand at 347.043 kb on - strand at 347.097 kb on - strand at 347.097 kb on - strand at 347.121 kb on - strand at 347.263 kb on + strand, within BWI76_RS01690 at 347.321 kb on + strand, within BWI76_RS01690 at 347.478 kb on - strand, within BWI76_RS01690 at 347.495 kb on - strand, within BWI76_RS01690 at 347.657 kb on - strand, within BWI76_RS01690 at 347.813 kb on - strand, within BWI76_RS01690
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 342,297 - BWI76_RS01680 0.21 +0.0 342,302 - BWI76_RS01680 0.21 -0.3 342,323 - BWI76_RS01680 0.24 -0.5 342,379 - BWI76_RS01680 0.30 -0.4 342,413 - BWI76_RS01680 0.35 -1.7 342,525 + BWI76_RS01680 0.48 +0.7 342,533 - BWI76_RS01680 0.49 -0.8 342,533 - BWI76_RS01680 0.49 -0.7 342,773 + BWI76_RS01680 0.78 -0.8 343,064 - -0.3 343,148 + -1.0 343,148 + -0.1 343,148 + -1.5 343,332 - -2.0 343,406 - -1.0 343,456 + -1.2 343,461 + -2.2 343,498 + -1.4 343,606 + BWI76_RS01685 0.12 +0.2 343,627 + BWI76_RS01685 0.13 -0.7 343,646 - BWI76_RS01685 0.13 -1.1 343,880 + BWI76_RS01685 0.20 -0.9 343,930 - BWI76_RS01685 0.21 -1.0 344,059 + BWI76_RS01685 0.25 -1.4 344,495 - BWI76_RS01685 0.36 -1.5 344,500 - BWI76_RS01685 0.37 -1.5 344,505 - BWI76_RS01685 0.37 -1.2 344,523 + BWI76_RS01685 0.37 -0.9 344,691 - BWI76_RS01685 0.42 -3.2 344,742 + BWI76_RS01685 0.43 -1.3 344,742 + BWI76_RS01685 0.43 -1.3 344,742 + BWI76_RS01685 0.43 -1.1 344,902 - BWI76_RS01685 0.48 -1.0 345,344 + BWI76_RS01685 0.60 -1.5 345,350 - BWI76_RS01685 0.60 -0.5 345,350 - BWI76_RS01685 0.60 +0.7 345,702 - BWI76_RS01685 0.69 -2.2 345,702 - BWI76_RS01685 0.69 +0.1 345,702 - BWI76_RS01685 0.69 -1.5 345,713 - BWI76_RS01685 0.70 -0.3 345,938 + BWI76_RS01685 0.76 -1.5 345,971 - +0.2 346,016 - BWI76_RS01685 0.78 -1.5 346,086 - BWI76_RS01685 0.80 -1.1 346,116 - BWI76_RS01685 0.80 -1.9 346,329 + BWI76_RS01685 0.86 -1.4 346,413 - BWI76_RS01685 0.89 -0.7 346,575 + -0.1 346,610 - -0.4 346,756 - -1.1 346,872 + -0.7 346,909 + -1.1 346,909 + -0.0 346,910 - -0.5 346,983 - -0.5 347,029 + -0.4 347,043 - -0.6 347,097 - -0.8 347,097 - -0.1 347,121 - -0.7 347,263 + BWI76_RS01690 0.15 +1.2 347,321 + BWI76_RS01690 0.18 -0.1 347,478 - BWI76_RS01690 0.28 +2.5 347,495 - BWI76_RS01690 0.29 -0.8 347,657 - BWI76_RS01690 0.39 -0.2 347,813 - BWI76_RS01690 0.49 +1.7
Or see this region's nucleotide sequence