Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00825 and BWI76_RS00830 are separated by 151 nucleotides BWI76_RS00830 and BWI76_RS00835 are separated by 97 nucleotides BWI76_RS00835 and BWI76_RS00840 are separated by 254 nucleotides
BWI76_RS00825: BWI76_RS00825 - AraC family transcriptional regulator, at 168,919 to 169,782
_RS00825
BWI76_RS00830: BWI76_RS00830 - metal-binding protein ZinT, at 169,934 to 170,581
_RS00830
BWI76_RS00835: BWI76_RS00835 - phosphoenolpyruvate carboxylase, at 170,679 to 173,330
_RS00835
BWI76_RS00840: BWI76_RS00840 - acetylornithine deacetylase, at 173,585 to 174,736
_RS00840
Position (kb) 170
171
172
173
174 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 169.825 kb on + strand at 169.843 kb on - strand at 169.919 kb on + strand at 169.927 kb on - strand at 169.982 kb on - strand at 170.030 kb on - strand, within BWI76_RS00830 at 170.082 kb on - strand, within BWI76_RS00830 at 170.235 kb on - strand, within BWI76_RS00830 at 170.241 kb on + strand, within BWI76_RS00830 at 170.251 kb on + strand, within BWI76_RS00830 at 170.552 kb on - strand at 170.664 kb on - strand at 170.694 kb on - strand at 170.785 kb on + strand at 170.917 kb on - strand at 170.939 kb on + strand at 170.950 kb on + strand, within BWI76_RS00835 at 170.968 kb on - strand, within BWI76_RS00835 at 171.149 kb on - strand, within BWI76_RS00835 at 171.387 kb on - strand, within BWI76_RS00835 at 171.426 kb on + strand, within BWI76_RS00835 at 171.434 kb on - strand, within BWI76_RS00835 at 171.434 kb on - strand, within BWI76_RS00835 at 171.502 kb on - strand, within BWI76_RS00835 at 171.652 kb on + strand, within BWI76_RS00835 at 171.676 kb on + strand, within BWI76_RS00835 at 171.760 kb on + strand, within BWI76_RS00835 at 171.768 kb on - strand, within BWI76_RS00835 at 171.960 kb on + strand at 171.979 kb on - strand, within BWI76_RS00835 at 172.047 kb on + strand, within BWI76_RS00835 at 172.115 kb on + strand, within BWI76_RS00835 at 172.127 kb on + strand, within BWI76_RS00835 at 172.244 kb on - strand, within BWI76_RS00835 at 172.641 kb on + strand, within BWI76_RS00835 at 172.692 kb on - strand, within BWI76_RS00835 at 172.853 kb on + strand, within BWI76_RS00835 at 172.853 kb on + strand, within BWI76_RS00835 at 172.861 kb on - strand, within BWI76_RS00835 at 172.861 kb on - strand, within BWI76_RS00835 at 172.928 kb on + strand, within BWI76_RS00835 at 173.110 kb on - strand at 173.127 kb on - strand at 173.311 kb on + strand at 173.334 kb on + strand at 173.342 kb on - strand at 173.539 kb on + strand at 173.619 kb on - strand at 173.698 kb on - strand at 173.737 kb on + strand, within BWI76_RS00840 at 173.863 kb on + strand, within BWI76_RS00840 at 173.960 kb on - strand, within BWI76_RS00840 at 173.960 kb on - strand, within BWI76_RS00840 at 174.257 kb on + strand, within BWI76_RS00840
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 169,825 + -0.3 169,843 - -0.1 169,919 + -0.1 169,927 - +0.0 169,982 - -0.2 170,030 - BWI76_RS00830 0.15 -0.9 170,082 - BWI76_RS00830 0.23 -0.3 170,235 - BWI76_RS00830 0.46 -0.4 170,241 + BWI76_RS00830 0.47 -0.1 170,251 + BWI76_RS00830 0.49 +0.1 170,552 - +0.6 170,664 - -1.2 170,694 - -1.1 170,785 + -3.5 170,917 - -4.7 170,939 + -5.9 170,950 + BWI76_RS00835 0.10 -5.2 170,968 - BWI76_RS00835 0.11 -3.2 171,149 - BWI76_RS00835 0.18 -5.3 171,387 - BWI76_RS00835 0.27 -3.4 171,426 + BWI76_RS00835 0.28 -4.1 171,434 - BWI76_RS00835 0.28 -5.0 171,434 - BWI76_RS00835 0.28 -3.2 171,502 - BWI76_RS00835 0.31 -4.4 171,652 + BWI76_RS00835 0.37 -4.2 171,676 + BWI76_RS00835 0.38 -5.0 171,760 + BWI76_RS00835 0.41 -1.6 171,768 - BWI76_RS00835 0.41 -5.8 171,960 + -0.7 171,979 - BWI76_RS00835 0.49 -2.8 172,047 + BWI76_RS00835 0.52 -6.3 172,115 + BWI76_RS00835 0.54 -3.4 172,127 + BWI76_RS00835 0.55 -6.1 172,244 - BWI76_RS00835 0.59 -2.8 172,641 + BWI76_RS00835 0.74 -3.0 172,692 - BWI76_RS00835 0.76 -3.3 172,853 + BWI76_RS00835 0.82 -5.5 172,853 + BWI76_RS00835 0.82 -4.0 172,861 - BWI76_RS00835 0.82 -4.0 172,861 - BWI76_RS00835 0.82 -1.5 172,928 + BWI76_RS00835 0.85 -5.4 173,110 - -4.5 173,127 - -3.6 173,311 + -4.5 173,334 + -4.8 173,342 - -0.9 173,539 + -0.1 173,619 - -0.4 173,698 - +0.6 173,737 + BWI76_RS00840 0.13 -0.2 173,863 + BWI76_RS00840 0.24 -0.7 173,960 - BWI76_RS00840 0.33 -0.3 173,960 - BWI76_RS00840 0.33 -0.8 174,257 + BWI76_RS00840 0.58 -0.3
Or see this region's nucleotide sequence