Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00350 and BWI76_RS00355 are separated by 396 nucleotides BWI76_RS00355 and BWI76_RS00360 are separated by 139 nucleotides BWI76_RS00360 and BWI76_RS00365 are separated by 28 nucleotides
BWI76_RS00350: BWI76_RS00350 - acyltransferase, at 72,900 to 73,799
_RS00350
BWI76_RS00355: BWI76_RS00355 - DNA polymerase I, at 74,196 to 76,985
_RS00355
BWI76_RS00360: BWI76_RS00360 - hypothetical protein, at 77,125 to 77,306
_RS00360
BWI76_RS00365: BWI76_RS00365 - YihA family ribosome biogenesis GTP-binding protein, at 77,335 to 77,967
_RS00365
Position (kb) 74
75
76
77 Strain fitness (log2 ratio) -2
-1
0
1
2 at 73.221 kb on + strand, within BWI76_RS00350 at 73.238 kb on + strand, within BWI76_RS00350 at 73.250 kb on - strand, within BWI76_RS00350 at 73.255 kb on + strand, within BWI76_RS00350 at 73.256 kb on - strand, within BWI76_RS00350 at 73.274 kb on - strand, within BWI76_RS00350 at 73.274 kb on - strand, within BWI76_RS00350 at 73.279 kb on - strand, within BWI76_RS00350 at 73.286 kb on - strand, within BWI76_RS00350 at 73.317 kb on + strand, within BWI76_RS00350 at 73.340 kb on + strand, within BWI76_RS00350 at 73.348 kb on - strand, within BWI76_RS00350 at 73.392 kb on + strand, within BWI76_RS00350 at 73.474 kb on - strand, within BWI76_RS00350 at 73.491 kb on + strand, within BWI76_RS00350 at 73.491 kb on + strand, within BWI76_RS00350 at 73.499 kb on - strand, within BWI76_RS00350 at 73.501 kb on - strand, within BWI76_RS00350 at 73.520 kb on - strand, within BWI76_RS00350 at 73.520 kb on - strand, within BWI76_RS00350 at 73.520 kb on - strand, within BWI76_RS00350 at 73.521 kb on - strand, within BWI76_RS00350 at 73.566 kb on + strand, within BWI76_RS00350 at 73.566 kb on + strand, within BWI76_RS00350 at 73.574 kb on - strand, within BWI76_RS00350 at 73.580 kb on + strand, within BWI76_RS00350 at 73.588 kb on - strand, within BWI76_RS00350 at 73.599 kb on - strand, within BWI76_RS00350 at 73.600 kb on - strand, within BWI76_RS00350 at 73.610 kb on - strand, within BWI76_RS00350 at 73.619 kb on - strand at 73.625 kb on - strand, within BWI76_RS00350 at 73.627 kb on - strand, within BWI76_RS00350 at 73.703 kb on + strand, within BWI76_RS00350 at 73.754 kb on + strand at 73.754 kb on + strand at 73.762 kb on - strand at 73.824 kb on - strand at 73.837 kb on + strand at 73.837 kb on + strand at 73.845 kb on - strand at 73.845 kb on - strand at 73.918 kb on + strand at 73.926 kb on - strand at 73.944 kb on + strand at 73.962 kb on - strand at 74.072 kb on + strand at 74.080 kb on - strand at 74.080 kb on - strand at 74.091 kb on + strand at 75.445 kb on + strand, within BWI76_RS00355 at 76.113 kb on + strand, within BWI76_RS00355 at 76.465 kb on + strand, within BWI76_RS00355 at 77.143 kb on + strand at 77.308 kb on - strand at 77.380 kb on - strand at 77.381 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 73,221 + BWI76_RS00350 0.36 -0.2 73,238 + BWI76_RS00350 0.38 -0.3 73,250 - BWI76_RS00350 0.39 -0.1 73,255 + BWI76_RS00350 0.39 +0.0 73,256 - BWI76_RS00350 0.40 +0.2 73,274 - BWI76_RS00350 0.42 +0.2 73,274 - BWI76_RS00350 0.42 -0.5 73,279 - BWI76_RS00350 0.42 -0.1 73,286 - BWI76_RS00350 0.43 +0.8 73,317 + BWI76_RS00350 0.46 -0.9 73,340 + BWI76_RS00350 0.49 -0.1 73,348 - BWI76_RS00350 0.50 +0.0 73,392 + BWI76_RS00350 0.55 +0.3 73,474 - BWI76_RS00350 0.64 +0.2 73,491 + BWI76_RS00350 0.66 -0.3 73,491 + BWI76_RS00350 0.66 -1.3 73,499 - BWI76_RS00350 0.67 +0.2 73,501 - BWI76_RS00350 0.67 -0.4 73,520 - BWI76_RS00350 0.69 -0.1 73,520 - BWI76_RS00350 0.69 +0.2 73,520 - BWI76_RS00350 0.69 -0.2 73,521 - BWI76_RS00350 0.69 +0.2 73,566 + BWI76_RS00350 0.74 -0.7 73,566 + BWI76_RS00350 0.74 +0.2 73,574 - BWI76_RS00350 0.75 +0.2 73,580 + BWI76_RS00350 0.76 +0.1 73,588 - BWI76_RS00350 0.76 -1.1 73,599 - BWI76_RS00350 0.78 +0.0 73,600 - BWI76_RS00350 0.78 +0.1 73,610 - BWI76_RS00350 0.79 -0.9 73,619 - +0.7 73,625 - BWI76_RS00350 0.81 -0.3 73,627 - BWI76_RS00350 0.81 +0.3 73,703 + BWI76_RS00350 0.89 +1.2 73,754 + +0.1 73,754 + -0.6 73,762 - -1.1 73,824 - -0.2 73,837 + -0.5 73,837 + -0.3 73,845 - -0.0 73,845 - -0.0 73,918 + +0.7 73,926 - -0.4 73,944 + +0.4 73,962 - +0.3 74,072 + +2.4 74,080 - -0.0 74,080 - -0.7 74,091 + -1.9 75,445 + BWI76_RS00355 0.45 +0.0 76,113 + BWI76_RS00355 0.69 -1.3 76,465 + BWI76_RS00355 0.81 -1.2 77,143 + +0.2 77,308 - -0.2 77,380 - -0.5 77,381 - -0.5
Or see this region's nucleotide sequence