Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4492 and BT4493 are separated by 95 nucleotides BT4493 and BT4494 overlap by 38 nucleotides BT4494 and BT4495 are separated by 131 nucleotides BT4495 and BT4496 are separated by 100 nucleotides
BT4492: BT4492 - conserved hypothetical protein (NCBI ptt file), at 5,908,395 to 5,910,131
BT4492
BT4493: BT4493 - putative sugar nucleotide epimerase (NCBI ptt file), at 5,910,227 to 5,911,540
BT4493
BT4494: BT4494 - conserved hypothetical protein (NCBI ptt file), at 5,911,503 to 5,911,823
BT4494
BT4495: BT4495 - conserved hypothetical protein (NCBI ptt file), at 5,911,955 to 5,912,224
BT4495
BT4496: BT4496 - putative oxidoreductase (NCBI ptt file), at 5,912,325 to 5,913,056
BT4496
Position (kb)
5910
5911
5912 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 5909.228 kb on + strand, within BT4492 at 5909.238 kb on + strand, within BT4492 at 5909.351 kb on + strand, within BT4492 at 5909.372 kb on - strand, within BT4492 at 5909.372 kb on - strand, within BT4492 at 5909.405 kb on - strand, within BT4492 at 5909.416 kb on - strand, within BT4492 at 5909.441 kb on + strand, within BT4492 at 5909.442 kb on - strand, within BT4492 at 5909.442 kb on - strand, within BT4492 at 5909.524 kb on + strand, within BT4492 at 5909.525 kb on - strand, within BT4492 at 5909.534 kb on + strand, within BT4492 at 5909.558 kb on + strand, within BT4492 at 5909.564 kb on + strand, within BT4492 at 5909.624 kb on + strand, within BT4492 at 5909.660 kb on + strand, within BT4492 at 5909.661 kb on - strand, within BT4492 at 5909.661 kb on - strand, within BT4492 at 5909.702 kb on + strand, within BT4492 at 5909.729 kb on + strand, within BT4492 at 5909.741 kb on + strand, within BT4492 at 5909.748 kb on + strand, within BT4492 at 5909.897 kb on + strand, within BT4492 at 5909.897 kb on + strand, within BT4492 at 5909.899 kb on + strand, within BT4492 at 5909.982 kb on + strand at 5910.068 kb on + strand at 5910.069 kb on - strand at 5910.069 kb on - strand at 5910.102 kb on - strand at 5910.202 kb on - strand at 5910.203 kb on + strand at 5910.235 kb on - strand at 5910.260 kb on - strand at 5910.344 kb on - strand at 5910.344 kb on - strand at 5910.344 kb on - strand at 5910.391 kb on - strand, within BT4493 at 5910.546 kb on - strand, within BT4493 at 5910.577 kb on + strand, within BT4493 at 5910.578 kb on - strand, within BT4493 at 5910.791 kb on + strand, within BT4493 at 5910.793 kb on + strand, within BT4493 at 5910.794 kb on - strand, within BT4493 at 5910.896 kb on - strand, within BT4493 at 5911.054 kb on + strand, within BT4493 at 5911.090 kb on + strand, within BT4493 at 5911.118 kb on + strand, within BT4493 at 5911.118 kb on + strand, within BT4493 at 5911.119 kb on - strand, within BT4493 at 5911.193 kb on + strand, within BT4493 at 5911.196 kb on - strand, within BT4493 at 5911.196 kb on - strand, within BT4493 at 5911.240 kb on + strand, within BT4493 at 5911.240 kb on + strand, within BT4493 at 5911.359 kb on - strand, within BT4493 at 5911.359 kb on - strand, within BT4493 at 5911.386 kb on - strand, within BT4493 at 5911.392 kb on - strand, within BT4493 at 5911.421 kb on + strand at 5911.480 kb on - strand at 5911.502 kb on + strand at 5911.624 kb on + strand, within BT4494 at 5911.639 kb on + strand, within BT4494 at 5911.647 kb on + strand, within BT4494 at 5911.773 kb on + strand, within BT4494 at 5911.773 kb on + strand, within BT4494 at 5911.774 kb on - strand, within BT4494 at 5911.825 kb on + strand at 5911.826 kb on - strand at 5911.826 kb on - strand at 5911.829 kb on + strand at 5911.842 kb on - strand at 5911.898 kb on - strand at 5911.914 kb on + strand at 5912.015 kb on + strand, within BT4495 at 5912.022 kb on + strand, within BT4495 at 5912.122 kb on - strand, within BT4495 at 5912.128 kb on - strand, within BT4495 at 5912.137 kb on - strand, within BT4495 at 5912.239 kb on - strand at 5912.281 kb on - strand at 5912.287 kb on - strand at 5912.287 kb on - strand at 5912.314 kb on - strand at 5912.414 kb on + strand, within BT4496 at 5912.415 kb on - strand, within BT4496 at 5912.418 kb on + strand, within BT4496 at 5912.446 kb on - strand, within BT4496
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 5,909,228 + BT4492 0.48 -3.5 5,909,238 + BT4492 0.49 +2.7 5,909,351 + BT4492 0.55 -1.9 5,909,372 - BT4492 0.56 +1.1 5,909,372 - BT4492 0.56 +0.0 5,909,405 - BT4492 0.58 +0.1 5,909,416 - BT4492 0.59 -0.4 5,909,441 + BT4492 0.60 -2.7 5,909,442 - BT4492 0.60 -0.1 5,909,442 - BT4492 0.60 +1.1 5,909,524 + BT4492 0.65 -0.6 5,909,525 - BT4492 0.65 +4.0 5,909,534 + BT4492 0.66 +0.9 5,909,558 + BT4492 0.67 -0.9 5,909,564 + BT4492 0.67 +2.9 5,909,624 + BT4492 0.71 -2.1 5,909,660 + BT4492 0.73 -4.1 5,909,661 - BT4492 0.73 +1.7 5,909,661 - BT4492 0.73 -2.3 5,909,702 + BT4492 0.75 +1.4 5,909,729 + BT4492 0.77 -1.4 5,909,741 + BT4492 0.77 +0.9 5,909,748 + BT4492 0.78 -0.7 5,909,897 + BT4492 0.86 -2.4 5,909,897 + BT4492 0.86 +0.3 5,909,899 + BT4492 0.87 -1.1 5,909,982 + -0.1 5,910,068 + -3.9 5,910,069 - -0.1 5,910,069 - -1.5 5,910,102 - -2.7 5,910,202 - -2.2 5,910,203 + -0.2 5,910,235 - -1.2 5,910,260 - +3.8 5,910,344 - -0.8 5,910,344 - +0.4 5,910,344 - -0.2 5,910,391 - BT4493 0.12 +1.4 5,910,546 - BT4493 0.24 +2.0 5,910,577 + BT4493 0.27 -1.7 5,910,578 - BT4493 0.27 +0.8 5,910,791 + BT4493 0.43 -1.5 5,910,793 + BT4493 0.43 +0.8 5,910,794 - BT4493 0.43 +1.6 5,910,896 - BT4493 0.51 -1.1 5,911,054 + BT4493 0.63 +2.6 5,911,090 + BT4493 0.66 +1.2 5,911,118 + BT4493 0.68 +1.4 5,911,118 + BT4493 0.68 -0.5 5,911,119 - BT4493 0.68 -3.5 5,911,193 + BT4493 0.74 -4.5 5,911,196 - BT4493 0.74 +0.6 5,911,196 - BT4493 0.74 -0.5 5,911,240 + BT4493 0.77 -0.2 5,911,240 + BT4493 0.77 +2.3 5,911,359 - BT4493 0.86 -1.7 5,911,359 - BT4493 0.86 +2.4 5,911,386 - BT4493 0.88 +1.4 5,911,392 - BT4493 0.89 +1.2 5,911,421 + -1.3 5,911,480 - -2.3 5,911,502 + -2.6 5,911,624 + BT4494 0.38 -3.3 5,911,639 + BT4494 0.42 -0.2 5,911,647 + BT4494 0.45 -1.0 5,911,773 + BT4494 0.84 -2.7 5,911,773 + BT4494 0.84 -1.5 5,911,774 - BT4494 0.84 -1.9 5,911,825 + +0.6 5,911,826 - -0.2 5,911,826 - -3.3 5,911,829 + +1.3 5,911,842 - -2.0 5,911,898 - -1.7 5,911,914 + -1.7 5,912,015 + BT4495 0.22 +0.8 5,912,022 + BT4495 0.25 -0.8 5,912,122 - BT4495 0.62 +0.6 5,912,128 - BT4495 0.64 +0.8 5,912,137 - BT4495 0.67 +1.6 5,912,239 - -0.2 5,912,281 - -4.1 5,912,287 - +1.3 5,912,287 - -2.5 5,912,314 - -2.4 5,912,414 + BT4496 0.12 -1.7 5,912,415 - BT4496 0.12 -2.2 5,912,418 + BT4496 0.13 -0.2 5,912,446 - BT4496 0.17 +1.4
Or see this region's nucleotide sequence