Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4216 and BT4217 are separated by 2 nucleotides BT4217 and BT4218 are separated by 46 nucleotides BT4218 and BT4219 are separated by 90 nucleotides BT4219 and BT4220 are separated by 83 nucleotides
BT4216: BT4216 - ubiquinone/menaquinone biosynthesis methyltransferase (NCBI ptt file), at 5,552,878 to 5,553,615
BT4216
BT4217: BT4217 - phosphoribosylamidoimidazole-succinocarboxamide synthase (NCBI ptt file), at 5,553,618 to 5,554,562
BT4217
BT4218: BT4218 - phosphate starvation-inducible protein, PhoH (NCBI ptt file), at 5,554,609 to 5,555,625
BT4218
BT4219: BT4219 - hypothetical protein (NCBI ptt file), at 5,555,716 to 5,556,393
BT4219
BT4220: BT4220 - flotillin-like protein (NCBI ptt file), at 5,556,477 to 5,558,135
BT4220
Position (kb)
5554
5555
5556 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5553.620 kb on - strand at 5553.932 kb on - strand, within BT4217 at 5553.936 kb on - strand, within BT4217 at 5554.718 kb on - strand, within BT4218 at 5554.858 kb on - strand, within BT4218 at 5554.930 kb on - strand, within BT4218 at 5554.984 kb on - strand, within BT4218 at 5555.070 kb on - strand, within BT4218 at 5555.072 kb on - strand, within BT4218 at 5555.085 kb on - strand, within BT4218 at 5555.255 kb on - strand, within BT4218 at 5555.386 kb on - strand, within BT4218 at 5555.525 kb on - strand at 5555.537 kb on - strand at 5555.537 kb on - strand at 5555.537 kb on - strand at 5555.549 kb on - strand at 5555.701 kb on + strand at 5555.701 kb on + strand at 5555.757 kb on - strand at 5555.762 kb on - strand at 5555.762 kb on - strand at 5555.980 kb on + strand, within BT4219 at 5556.089 kb on + strand, within BT4219 at 5556.116 kb on - strand, within BT4219 at 5556.257 kb on + strand, within BT4219 at 5556.257 kb on + strand, within BT4219 at 5556.258 kb on - strand, within BT4219 at 5556.306 kb on + strand, within BT4219 at 5556.358 kb on - strand at 5556.358 kb on - strand at 5556.382 kb on - strand at 5556.460 kb on - strand at 5556.460 kb on - strand at 5556.592 kb on - strand at 5556.592 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 5,553,620 - +2.3 5,553,932 - BT4217 0.33 -0.0 5,553,936 - BT4217 0.34 -0.0 5,554,718 - BT4218 0.11 +2.0 5,554,858 - BT4218 0.24 -2.6 5,554,930 - BT4218 0.32 +0.6 5,554,984 - BT4218 0.37 -2.6 5,555,070 - BT4218 0.45 -1.1 5,555,072 - BT4218 0.46 -2.3 5,555,085 - BT4218 0.47 +0.7 5,555,255 - BT4218 0.64 -2.7 5,555,386 - BT4218 0.76 -1.2 5,555,525 - -1.6 5,555,537 - -1.6 5,555,537 - -2.5 5,555,537 - -2.8 5,555,549 - -2.3 5,555,701 + -1.0 5,555,701 + -1.4 5,555,757 - -3.1 5,555,762 - -1.0 5,555,762 - -3.6 5,555,980 + BT4219 0.39 -1.0 5,556,089 + BT4219 0.55 -3.2 5,556,116 - BT4219 0.59 -0.0 5,556,257 + BT4219 0.80 -1.8 5,556,257 + BT4219 0.80 -1.8 5,556,258 - BT4219 0.80 -0.0 5,556,306 + BT4219 0.87 -1.6 5,556,358 - +0.5 5,556,358 - -2.8 5,556,382 - +1.8 5,556,460 - -2.0 5,556,460 - -1.2 5,556,592 - +1.6 5,556,592 - -1.6
Or see this region's nucleotide sequence