Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT4217

Experiment: BHIS with Chlorpromazine 0.1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT4215 and BT4216 are separated by 38 nucleotidesBT4216 and BT4217 are separated by 2 nucleotidesBT4217 and BT4218 are separated by 46 nucleotides BT4215: BT4215 - shikimate dehydrogenase (EC 1.1.1.25) (from data), at 5,552,093 to 5,552,839 BT4215 BT4216: BT4216 - ubiquinone/menaquinone biosynthesis methyltransferase (NCBI ptt file), at 5,552,878 to 5,553,615 BT4216 BT4217: BT4217 - phosphoribosylamidoimidazole-succinocarboxamide synthase (NCBI ptt file), at 5,553,618 to 5,554,562 BT4217 BT4218: BT4218 - phosphate starvation-inducible protein, PhoH (NCBI ptt file), at 5,554,609 to 5,555,625 BT4218 Position (kb) 5553 5554 5555Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5552.863 kb on - strandat 5552.863 kb on - strandat 5553.595 kb on - strandat 5553.620 kb on - strandat 5553.932 kb on - strand, within BT4217at 5553.936 kb on - strand, within BT4217at 5554.718 kb on - strand, within BT4218at 5554.858 kb on - strand, within BT4218at 5554.930 kb on - strand, within BT4218at 5554.984 kb on - strand, within BT4218at 5555.070 kb on - strand, within BT4218at 5555.072 kb on - strand, within BT4218at 5555.085 kb on - strand, within BT4218at 5555.255 kb on - strand, within BT4218at 5555.386 kb on - strand, within BT4218at 5555.525 kb on - strandat 5555.537 kb on - strandat 5555.537 kb on - strandat 5555.537 kb on - strandat 5555.549 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM
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5,552,863 - -0.5
5,552,863 - +1.3
5,553,595 - -3.2
5,553,620 - +2.3
5,553,932 - BT4217 0.33 -0.0
5,553,936 - BT4217 0.34 -0.0
5,554,718 - BT4218 0.11 +2.0
5,554,858 - BT4218 0.24 -2.6
5,554,930 - BT4218 0.32 +0.6
5,554,984 - BT4218 0.37 -2.6
5,555,070 - BT4218 0.45 -1.1
5,555,072 - BT4218 0.46 -2.3
5,555,085 - BT4218 0.47 +0.7
5,555,255 - BT4218 0.64 -2.7
5,555,386 - BT4218 0.76 -1.2
5,555,525 - -1.6
5,555,537 - -1.6
5,555,537 - -2.5
5,555,537 - -2.8
5,555,549 - -2.3

Or see this region's nucleotide sequence