Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3888 and BT3889 are separated by 24 nucleotides BT3889 and BT3890 are separated by 89 nucleotides
BT3888: BT3888 - putative tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase (NCBI ptt file), at 5,058,844 to 5,059,899
BT3888
BT3889: BT3889 - subtilisin-like serine proteases (NCBI ptt file), at 5,059,924 to 5,061,279
BT3889
BT3890: BT3890 - putative exodeoxyribonuclease VII large subunit (NCBI ptt file), at 5,061,369 to 5,062,622
BT3890
Position (kb)
5059
5060
5061
5062 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 5059.907 kb on + strand at 5060.028 kb on + strand at 5060.028 kb on + strand at 5060.029 kb on - strand at 5060.045 kb on - strand at 5060.045 kb on - strand at 5060.150 kb on - strand, within BT3889 at 5060.154 kb on - strand, within BT3889 at 5060.236 kb on - strand, within BT3889 at 5060.236 kb on - strand, within BT3889 at 5060.548 kb on + strand, within BT3889 at 5060.569 kb on - strand, within BT3889 at 5060.577 kb on - strand, within BT3889 at 5060.788 kb on + strand, within BT3889 at 5060.789 kb on - strand, within BT3889 at 5060.955 kb on + strand, within BT3889 at 5060.956 kb on - strand, within BT3889 at 5060.956 kb on - strand, within BT3889 at 5061.057 kb on - strand, within BT3889 at 5061.111 kb on + strand, within BT3889 at 5061.112 kb on - strand, within BT3889 at 5061.112 kb on - strand, within BT3889 at 5061.226 kb on + strand at 5061.227 kb on - strand at 5061.260 kb on - strand at 5061.278 kb on - strand at 5061.306 kb on - strand at 5061.306 kb on - strand at 5061.332 kb on - strand at 5061.359 kb on - strand at 5061.362 kb on + strand at 5061.388 kb on + strand at 5061.394 kb on + strand at 5061.495 kb on + strand, within BT3890 at 5061.567 kb on - strand, within BT3890 at 5061.605 kb on + strand, within BT3890 at 5061.616 kb on + strand, within BT3890 at 5061.617 kb on - strand, within BT3890 at 5061.672 kb on + strand, within BT3890 at 5061.673 kb on - strand, within BT3890 at 5061.749 kb on - strand, within BT3890 at 5061.776 kb on - strand, within BT3890 at 5061.821 kb on + strand, within BT3890 at 5061.822 kb on - strand, within BT3890 at 5061.906 kb on + strand, within BT3890 at 5062.006 kb on + strand, within BT3890 at 5062.059 kb on - strand, within BT3890 at 5062.063 kb on - strand, within BT3890 at 5062.063 kb on - strand, within BT3890 at 5062.264 kb on - strand, within BT3890
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 5,059,907 + -2.5 5,060,028 + -2.1 5,060,028 + +0.1 5,060,029 - +2.7 5,060,045 - -0.9 5,060,045 - -1.2 5,060,150 - BT3889 0.17 -0.8 5,060,154 - BT3889 0.17 -2.6 5,060,236 - BT3889 0.23 -1.9 5,060,236 - BT3889 0.23 -2.9 5,060,548 + BT3889 0.46 -0.9 5,060,569 - BT3889 0.48 -0.5 5,060,577 - BT3889 0.48 -3.6 5,060,788 + BT3889 0.64 +0.1 5,060,789 - BT3889 0.64 -1.1 5,060,955 + BT3889 0.76 -2.5 5,060,956 - BT3889 0.76 +0.5 5,060,956 - BT3889 0.76 +0.1 5,061,057 - BT3889 0.84 -2.2 5,061,111 + BT3889 0.88 -3.7 5,061,112 - BT3889 0.88 -1.6 5,061,112 - BT3889 0.88 -1.1 5,061,226 + +0.1 5,061,227 - +0.3 5,061,260 - -0.7 5,061,278 - -1.7 5,061,306 - +0.1 5,061,306 - +1.1 5,061,332 - -2.1 5,061,359 - +2.5 5,061,362 + +1.7 5,061,388 + -0.9 5,061,394 + -3.9 5,061,495 + BT3890 0.10 +1.0 5,061,567 - BT3890 0.16 +1.0 5,061,605 + BT3890 0.19 -1.5 5,061,616 + BT3890 0.20 +2.9 5,061,617 - BT3890 0.20 -2.2 5,061,672 + BT3890 0.24 -2.5 5,061,673 - BT3890 0.24 -5.6 5,061,749 - BT3890 0.30 +0.1 5,061,776 - BT3890 0.32 +1.4 5,061,821 + BT3890 0.36 -0.4 5,061,822 - BT3890 0.36 +1.6 5,061,906 + BT3890 0.43 -1.7 5,062,006 + BT3890 0.51 +0.4 5,062,059 - BT3890 0.55 -1.5 5,062,063 - BT3890 0.55 -1.5 5,062,063 - BT3890 0.55 -2.1 5,062,264 - BT3890 0.71 -1.2
Or see this region's nucleotide sequence