Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3352 and BT3353 are separated by 115 nucleotides BT3353 and BT3354 are separated by 52 nucleotides
BT3352: BT3352 - haloacid dehalogenase-like hydrolase (NCBI ptt file), at 4,323,799 to 4,324,611
BT3352
BT3353: BT3353 - Snf2 family helicase (NCBI ptt file), at 4,324,727 to 4,327,810
BT3353
BT3354: BT3354 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (NCBI ptt file), at 4,327,863 to 4,328,975
BT3354
Position (kb)
4324
4325
4326
4327
4328 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4323.861 kb on + strand at 4323.911 kb on - strand, within BT3352 at 4323.950 kb on + strand, within BT3352 at 4323.950 kb on + strand, within BT3352 at 4323.963 kb on + strand, within BT3352 at 4323.963 kb on + strand, within BT3352 at 4323.964 kb on - strand, within BT3352 at 4323.968 kb on - strand, within BT3352 at 4324.019 kb on - strand, within BT3352 at 4324.118 kb on - strand, within BT3352 at 4324.127 kb on - strand, within BT3352 at 4324.241 kb on + strand, within BT3352 at 4324.242 kb on - strand, within BT3352 at 4324.407 kb on - strand, within BT3352 at 4324.407 kb on - strand, within BT3352 at 4324.407 kb on - strand, within BT3352 at 4324.416 kb on + strand, within BT3352 at 4324.460 kb on - strand, within BT3352 at 4324.469 kb on + strand, within BT3352 at 4324.469 kb on + strand, within BT3352 at 4324.516 kb on + strand, within BT3352 at 4324.522 kb on + strand, within BT3352 at 4324.605 kb on - strand at 4324.673 kb on + strand at 4324.709 kb on + strand at 4324.710 kb on - strand at 4324.849 kb on + strand at 4324.958 kb on - strand at 4324.979 kb on - strand at 4325.097 kb on + strand, within BT3353 at 4325.133 kb on - strand, within BT3353 at 4325.174 kb on - strand, within BT3353 at 4325.190 kb on - strand, within BT3353 at 4325.314 kb on + strand, within BT3353 at 4325.314 kb on + strand, within BT3353 at 4325.315 kb on - strand, within BT3353 at 4325.320 kb on - strand, within BT3353 at 4325.351 kb on + strand, within BT3353 at 4325.352 kb on - strand, within BT3353 at 4325.480 kb on - strand, within BT3353 at 4325.546 kb on - strand, within BT3353 at 4325.647 kb on + strand, within BT3353 at 4325.736 kb on - strand, within BT3353 at 4325.805 kb on - strand, within BT3353 at 4325.869 kb on - strand, within BT3353 at 4326.069 kb on - strand, within BT3353 at 4326.127 kb on + strand, within BT3353 at 4326.223 kb on + strand, within BT3353 at 4326.224 kb on - strand, within BT3353 at 4326.224 kb on - strand, within BT3353 at 4326.224 kb on - strand at 4326.225 kb on + strand, within BT3353 at 4326.225 kb on + strand, within BT3353 at 4326.311 kb on + strand, within BT3353 at 4326.312 kb on - strand, within BT3353 at 4326.386 kb on + strand, within BT3353 at 4326.513 kb on + strand at 4326.643 kb on + strand, within BT3353 at 4326.648 kb on - strand, within BT3353 at 4326.648 kb on - strand, within BT3353 at 4326.708 kb on + strand, within BT3353 at 4326.770 kb on - strand, within BT3353 at 4327.024 kb on + strand, within BT3353 at 4327.056 kb on + strand, within BT3353 at 4327.134 kb on + strand, within BT3353 at 4327.208 kb on - strand, within BT3353 at 4327.289 kb on + strand, within BT3353 at 4327.410 kb on + strand, within BT3353 at 4327.453 kb on + strand, within BT3353 at 4327.702 kb on - strand at 4327.729 kb on + strand at 4327.729 kb on + strand at 4327.731 kb on + strand at 4327.825 kb on - strand at 4327.838 kb on - strand at 4327.885 kb on + strand at 4328.058 kb on + strand, within BT3354 at 4328.059 kb on - strand, within BT3354 at 4328.113 kb on - strand, within BT3354 at 4328.115 kb on - strand, within BT3354 at 4328.115 kb on - strand at 4328.153 kb on + strand, within BT3354 at 4328.260 kb on - strand, within BT3354 at 4328.268 kb on + strand, within BT3354 at 4328.360 kb on + strand, within BT3354 at 4328.597 kb on - strand, within BT3354 at 4328.766 kb on - strand, within BT3354 at 4328.801 kb on + strand, within BT3354
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 4,323,861 + -2.6 4,323,911 - BT3352 0.14 +1.4 4,323,950 + BT3352 0.19 -0.9 4,323,950 + BT3352 0.19 -0.9 4,323,963 + BT3352 0.20 +0.1 4,323,963 + BT3352 0.20 +1.8 4,323,964 - BT3352 0.20 +2.2 4,323,968 - BT3352 0.21 -0.1 4,324,019 - BT3352 0.27 -2.4 4,324,118 - BT3352 0.39 -2.8 4,324,127 - BT3352 0.40 +3.4 4,324,241 + BT3352 0.54 -0.4 4,324,242 - BT3352 0.54 -1.4 4,324,407 - BT3352 0.75 +1.5 4,324,407 - BT3352 0.75 -1.9 4,324,407 - BT3352 0.75 +0.2 4,324,416 + BT3352 0.76 -1.1 4,324,460 - BT3352 0.81 +0.2 4,324,469 + BT3352 0.82 -1.8 4,324,469 + BT3352 0.82 +1.2 4,324,516 + BT3352 0.88 +2.4 4,324,522 + BT3352 0.89 -2.8 4,324,605 - +1.8 4,324,673 + -0.8 4,324,709 + -4.6 4,324,710 - -1.1 4,324,849 + -1.4 4,324,958 - +0.2 4,324,979 - -2.1 4,325,097 + BT3353 0.12 -1.5 4,325,133 - BT3353 0.13 -1.8 4,325,174 - BT3353 0.14 -2.2 4,325,190 - BT3353 0.15 +2.8 4,325,314 + BT3353 0.19 -1.8 4,325,314 + BT3353 0.19 -1.0 4,325,315 - BT3353 0.19 +0.2 4,325,320 - BT3353 0.19 -1.7 4,325,351 + BT3353 0.20 -0.8 4,325,352 - BT3353 0.20 +0.2 4,325,480 - BT3353 0.24 -1.3 4,325,546 - BT3353 0.27 -2.9 4,325,647 + BT3353 0.30 +2.2 4,325,736 - BT3353 0.33 -1.8 4,325,805 - BT3353 0.35 -0.1 4,325,869 - BT3353 0.37 -1.8 4,326,069 - BT3353 0.44 -1.1 4,326,127 + BT3353 0.45 +0.2 4,326,223 + BT3353 0.49 +2.0 4,326,224 - BT3353 0.49 +0.2 4,326,224 - BT3353 0.49 +0.6 4,326,224 - -2.1 4,326,225 + BT3353 0.49 -2.1 4,326,225 + BT3353 0.49 -1.5 4,326,311 + BT3353 0.51 +0.2 4,326,312 - BT3353 0.51 -1.8 4,326,386 + BT3353 0.54 +0.0 4,326,513 + +0.2 4,326,643 + BT3353 0.62 -2.8 4,326,648 - BT3353 0.62 +0.1 4,326,648 - BT3353 0.62 +0.2 4,326,708 + BT3353 0.64 +3.6 4,326,770 - BT3353 0.66 -1.1 4,327,024 + BT3353 0.74 +1.2 4,327,056 + BT3353 0.76 -1.8 4,327,134 + BT3353 0.78 -0.8 4,327,208 - BT3353 0.80 +3.0 4,327,289 + BT3353 0.83 -2.4 4,327,410 + BT3353 0.87 -1.7 4,327,453 + BT3353 0.88 -2.4 4,327,702 - -1.5 4,327,729 + -2.6 4,327,729 + -2.6 4,327,731 + +2.5 4,327,825 - +0.8 4,327,838 - +0.2 4,327,885 + +0.9 4,328,058 + BT3354 0.18 -0.7 4,328,059 - BT3354 0.18 +0.2 4,328,113 - BT3354 0.22 -2.8 4,328,115 - BT3354 0.23 -0.8 4,328,115 - -2.8 4,328,153 + BT3354 0.26 -3.0 4,328,260 - BT3354 0.36 -2.8 4,328,268 + BT3354 0.36 +0.8 4,328,360 + BT3354 0.45 +0.2 4,328,597 - BT3354 0.66 -1.2 4,328,766 - BT3354 0.81 +1.0 4,328,801 + BT3354 0.84 +0.2
Or see this region's nucleotide sequence