Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3073 and BT3074 are separated by 2 nucleotides BT3074 and BT3075 overlap by 10 nucleotides BT3075 and BT3076 are separated by 109 nucleotides BT3076 and BT3077 overlap by 41 nucleotides
BT3073: BT3073 - putative TIM-barrel enzyme, possible dehydrogenase, contains a highly conserved dihydrouridine synthase domain (NCBI ptt file), at 3,897,137 to 3,898,129
BT3073
BT3074: BT3074 - conserved hypothetical protein (NCBI ptt file), at 3,898,132 to 3,899,097
BT3074
BT3075: BT3075 - NAD-dependent epimerase/dehydratase family protein (NCBI ptt file), at 3,899,088 to 3,900,095
BT3075
BT3076: BT3076 - hypothetical protein (NCBI ptt file), at 3,900,205 to 3,900,399
BT3076
BT3077: BT3077 - ribonuclease R (NCBI ptt file), at 3,900,359 to 3,902,515
BT3077
Position (kb)
3899
3900
3901 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 3898.134 kb on - strand at 3898.138 kb on - strand at 3898.188 kb on - strand at 3898.248 kb on - strand, within BT3074 at 3898.249 kb on + strand, within BT3074 at 3898.250 kb on - strand, within BT3074 at 3898.274 kb on + strand, within BT3074 at 3898.323 kb on + strand, within BT3074 at 3898.330 kb on - strand, within BT3074 at 3898.450 kb on + strand, within BT3074 at 3898.451 kb on - strand, within BT3074 at 3898.577 kb on + strand, within BT3074 at 3898.578 kb on - strand, within BT3074 at 3898.581 kb on + strand, within BT3074 at 3898.601 kb on - strand, within BT3074 at 3898.639 kb on - strand, within BT3074 at 3898.677 kb on - strand, within BT3074 at 3898.743 kb on + strand, within BT3074 at 3898.743 kb on + strand, within BT3074 at 3898.764 kb on + strand, within BT3074 at 3898.779 kb on + strand, within BT3074 at 3899.025 kb on - strand at 3899.036 kb on - strand at 3899.083 kb on + strand at 3899.084 kb on - strand at 3899.149 kb on + strand at 3899.162 kb on - strand at 3899.166 kb on + strand at 3899.187 kb on + strand at 3899.187 kb on + strand at 3899.187 kb on + strand at 3899.188 kb on - strand at 3899.205 kb on - strand, within BT3075 at 3899.205 kb on - strand, within BT3075 at 3899.205 kb on - strand, within BT3075 at 3899.244 kb on - strand, within BT3075 at 3899.292 kb on - strand, within BT3075 at 3899.299 kb on - strand, within BT3075 at 3899.307 kb on + strand, within BT3075 at 3899.327 kb on + strand, within BT3075 at 3899.327 kb on + strand, within BT3075 at 3899.328 kb on - strand, within BT3075 at 3899.358 kb on + strand, within BT3075 at 3899.358 kb on + strand, within BT3075 at 3899.370 kb on + strand, within BT3075 at 3899.381 kb on + strand, within BT3075 at 3899.381 kb on + strand, within BT3075 at 3899.382 kb on - strand, within BT3075 at 3899.383 kb on + strand, within BT3075 at 3899.384 kb on - strand, within BT3075 at 3899.384 kb on - strand, within BT3075 at 3899.384 kb on - strand, within BT3075 at 3899.417 kb on + strand, within BT3075 at 3899.467 kb on - strand, within BT3075 at 3899.467 kb on - strand, within BT3075 at 3899.570 kb on - strand, within BT3075 at 3899.570 kb on - strand, within BT3075 at 3899.579 kb on + strand, within BT3075 at 3899.580 kb on - strand, within BT3075 at 3899.580 kb on - strand, within BT3075 at 3899.587 kb on + strand, within BT3075 at 3899.591 kb on + strand, within BT3075 at 3899.637 kb on - strand, within BT3075 at 3899.645 kb on + strand, within BT3075 at 3899.646 kb on - strand, within BT3075 at 3899.653 kb on + strand, within BT3075 at 3899.660 kb on + strand, within BT3075 at 3899.688 kb on + strand, within BT3075 at 3899.698 kb on + strand, within BT3075 at 3899.735 kb on + strand, within BT3075 at 3899.743 kb on + strand, within BT3075 at 3899.766 kb on - strand, within BT3075 at 3899.783 kb on + strand, within BT3075 at 3899.789 kb on + strand, within BT3075 at 3899.789 kb on + strand, within BT3075 at 3899.809 kb on + strand, within BT3075 at 3899.818 kb on + strand, within BT3075 at 3899.818 kb on + strand, within BT3075 at 3899.818 kb on + strand, within BT3075 at 3899.870 kb on + strand, within BT3075 at 3899.871 kb on - strand, within BT3075 at 3899.882 kb on + strand, within BT3075 at 3899.883 kb on - strand, within BT3075 at 3899.887 kb on + strand, within BT3075 at 3899.888 kb on - strand, within BT3075 at 3899.978 kb on - strand, within BT3075 at 3900.030 kb on + strand at 3900.082 kb on - strand at 3900.082 kb on - strand at 3900.114 kb on - strand at 3900.114 kb on - strand at 3900.146 kb on - strand at 3900.146 kb on - strand at 3900.146 kb on - strand at 3900.146 kb on - strand at 3900.216 kb on + strand at 3900.216 kb on + strand at 3900.217 kb on - strand at 3900.217 kb on - strand at 3900.253 kb on - strand, within BT3076 at 3900.353 kb on + strand, within BT3076
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 3,898,134 - +0.5 3,898,138 - -1.2 3,898,188 - -0.8 3,898,248 - BT3074 0.12 -4.6 3,898,249 + BT3074 0.12 +0.2 3,898,250 - BT3074 0.12 +0.2 3,898,274 + BT3074 0.15 +0.2 3,898,323 + BT3074 0.20 -4.0 3,898,330 - BT3074 0.20 -5.2 3,898,450 + BT3074 0.33 -2.8 3,898,451 - BT3074 0.33 +0.2 3,898,577 + BT3074 0.46 -0.8 3,898,578 - BT3074 0.46 -2.6 3,898,581 + BT3074 0.46 +0.2 3,898,601 - BT3074 0.49 -2.1 3,898,639 - BT3074 0.52 +0.2 3,898,677 - BT3074 0.56 -1.8 3,898,743 + BT3074 0.63 -3.5 3,898,743 + BT3074 0.63 -0.8 3,898,764 + BT3074 0.65 -3.9 3,898,779 + BT3074 0.67 -2.4 3,899,025 - +0.2 3,899,036 - -1.8 3,899,083 + -2.8 3,899,084 - +0.2 3,899,149 + -2.7 3,899,162 - -3.0 3,899,166 + -3.4 3,899,187 + -0.8 3,899,187 + -1.8 3,899,187 + -1.4 3,899,188 - -0.8 3,899,205 - BT3075 0.12 -1.1 3,899,205 - BT3075 0.12 -4.0 3,899,205 - BT3075 0.12 -4.6 3,899,244 - BT3075 0.15 -2.4 3,899,292 - BT3075 0.20 -2.7 3,899,299 - BT3075 0.21 -2.8 3,899,307 + BT3075 0.22 -5.2 3,899,327 + BT3075 0.24 -4.4 3,899,327 + BT3075 0.24 -3.9 3,899,328 - BT3075 0.24 -1.2 3,899,358 + BT3075 0.27 -0.4 3,899,358 + BT3075 0.27 -1.4 3,899,370 + BT3075 0.28 -1.4 3,899,381 + BT3075 0.29 -0.7 3,899,381 + BT3075 0.29 -4.4 3,899,382 - BT3075 0.29 -0.5 3,899,383 + BT3075 0.29 -3.0 3,899,384 - BT3075 0.29 -1.5 3,899,384 - BT3075 0.29 -2.4 3,899,384 - BT3075 0.29 -1.6 3,899,417 + BT3075 0.33 -3.4 3,899,467 - BT3075 0.38 -1.4 3,899,467 - BT3075 0.38 -3.9 3,899,570 - BT3075 0.48 -2.8 3,899,570 - BT3075 0.48 -0.1 3,899,579 + BT3075 0.49 +3.3 3,899,580 - BT3075 0.49 -1.1 3,899,580 - BT3075 0.49 -3.0 3,899,587 + BT3075 0.50 -0.9 3,899,591 + BT3075 0.50 -3.0 3,899,637 - BT3075 0.54 -1.4 3,899,645 + BT3075 0.55 -0.8 3,899,646 - BT3075 0.55 -3.0 3,899,653 + BT3075 0.56 -1.4 3,899,660 + BT3075 0.57 -2.8 3,899,688 + BT3075 0.60 +0.3 3,899,698 + BT3075 0.61 -1.8 3,899,735 + BT3075 0.64 -2.2 3,899,743 + BT3075 0.65 -2.1 3,899,766 - BT3075 0.67 -3.0 3,899,783 + BT3075 0.69 -3.6 3,899,789 + BT3075 0.70 -3.7 3,899,789 + BT3075 0.70 -3.4 3,899,809 + BT3075 0.72 -1.6 3,899,818 + BT3075 0.72 -4.0 3,899,818 + BT3075 0.72 -0.6 3,899,818 + BT3075 0.72 -2.1 3,899,870 + BT3075 0.78 -3.1 3,899,871 - BT3075 0.78 -1.8 3,899,882 + BT3075 0.79 -3.6 3,899,883 - BT3075 0.79 -1.8 3,899,887 + BT3075 0.79 -4.1 3,899,888 - BT3075 0.79 -2.1 3,899,978 - BT3075 0.88 -1.8 3,900,030 + -0.8 3,900,082 - -3.3 3,900,082 - -2.6 3,900,114 - +0.2 3,900,114 - +2.1 3,900,146 - +0.2 3,900,146 - -2.6 3,900,146 - +1.2 3,900,146 - -1.8 3,900,216 + -2.7 3,900,216 + -2.0 3,900,217 - -1.7 3,900,217 - -0.9 3,900,253 - BT3076 0.25 -1.4 3,900,353 + BT3076 0.76 -1.4
Or see this region's nucleotide sequence