Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2513 and BT2514 are separated by 43 nucleotides BT2514 and BT2515 are separated by 139 nucleotides BT2515 and BT2516 are separated by 68 nucleotides BT2516 and BT2517 are separated by 631 nucleotides
BT2513: BT2513 - conserved hypothetical protein (NCBI ptt file), at 3,137,994 to 3,139,433
BT2513
BT2514: BT2514 - hypothetical protein (NCBI ptt file), at 3,139,477 to 3,139,674
BT2514
BT2515: BT2515 - hypothetical protein (NCBI ptt file), at 3,139,814 to 3,139,963
BT2515
BT2516: BT2516 - conserved hypothetical protein (NCBI ptt file), at 3,140,032 to 3,140,724
BT2516
BT2517: BT2517 - GcpE, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (NCBI ptt file), at 3,141,356 to 3,143,197
BT2517
Position (kb)
3140
3141 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3139.052 kb on + strand, within BT2513 at 3139.100 kb on + strand, within BT2513 at 3139.100 kb on + strand, within BT2513 at 3139.113 kb on + strand, within BT2513 at 3139.135 kb on + strand, within BT2513 at 3139.135 kb on + strand, within BT2513 at 3139.136 kb on - strand, within BT2513 at 3139.165 kb on - strand, within BT2513 at 3139.170 kb on - strand, within BT2513 at 3139.190 kb on - strand, within BT2513 at 3139.190 kb on - strand, within BT2513 at 3139.216 kb on - strand, within BT2513 at 3139.251 kb on - strand, within BT2513 at 3139.264 kb on - strand, within BT2513 at 3139.359 kb on + strand at 3139.359 kb on + strand at 3139.415 kb on + strand at 3139.416 kb on - strand at 3139.431 kb on + strand at 3139.446 kb on + strand at 3139.544 kb on + strand, within BT2514 at 3139.581 kb on - strand, within BT2514 at 3139.613 kb on + strand, within BT2514 at 3139.613 kb on + strand, within BT2514 at 3139.614 kb on - strand, within BT2514 at 3139.614 kb on - strand, within BT2514 at 3139.614 kb on - strand, within BT2514 at 3139.614 kb on - strand, within BT2514 at 3139.660 kb on + strand at 3139.661 kb on - strand at 3139.670 kb on + strand at 3139.704 kb on - strand at 3139.722 kb on - strand at 3139.724 kb on - strand at 3139.728 kb on - strand at 3139.738 kb on - strand at 3139.746 kb on + strand at 3139.747 kb on - strand at 3139.771 kb on + strand at 3139.772 kb on - strand at 3139.824 kb on + strand at 3139.824 kb on + strand at 3139.836 kb on - strand, within BT2515 at 3139.838 kb on - strand, within BT2515 at 3139.839 kb on + strand, within BT2515 at 3139.867 kb on + strand, within BT2515 at 3139.890 kb on - strand, within BT2515 at 3139.900 kb on + strand, within BT2515 at 3139.900 kb on + strand, within BT2515 at 3140.032 kb on - strand at 3140.092 kb on - strand at 3140.103 kb on + strand, within BT2516 at 3140.103 kb on + strand, within BT2516 at 3140.125 kb on + strand, within BT2516 at 3140.126 kb on - strand, within BT2516 at 3140.126 kb on - strand, within BT2516 at 3140.214 kb on - strand, within BT2516 at 3140.217 kb on + strand, within BT2516 at 3140.230 kb on - strand, within BT2516 at 3140.265 kb on - strand, within BT2516 at 3140.312 kb on + strand, within BT2516 at 3140.325 kb on + strand, within BT2516 at 3140.412 kb on + strand, within BT2516 at 3140.415 kb on - strand, within BT2516 at 3140.418 kb on - strand, within BT2516 at 3140.430 kb on - strand, within BT2516 at 3140.448 kb on - strand, within BT2516 at 3140.477 kb on + strand, within BT2516 at 3140.478 kb on - strand, within BT2516 at 3140.478 kb on - strand, within BT2516 at 3140.543 kb on + strand, within BT2516 at 3140.544 kb on - strand, within BT2516 at 3140.622 kb on - strand, within BT2516 at 3140.622 kb on - strand, within BT2516 at 3140.622 kb on - strand, within BT2516 at 3140.722 kb on + strand at 3140.731 kb on + strand at 3140.794 kb on - strand at 3140.803 kb on - strand at 3140.803 kb on - strand at 3140.826 kb on + strand at 3140.827 kb on - strand at 3140.849 kb on - strand at 3140.849 kb on - strand at 3140.852 kb on - strand at 3140.892 kb on + strand at 3140.912 kb on - strand at 3140.938 kb on - strand at 3140.972 kb on + strand at 3140.979 kb on + strand at 3140.979 kb on + strand at 3140.981 kb on + strand at 3140.981 kb on + strand at 3140.982 kb on - strand at 3140.983 kb on + strand at 3140.983 kb on + strand at 3140.983 kb on + strand at 3140.984 kb on - strand at 3140.984 kb on - strand at 3140.989 kb on - strand at 3140.994 kb on - strand at 3141.008 kb on - strand at 3141.019 kb on + strand at 3141.019 kb on + strand at 3141.019 kb on + strand at 3141.036 kb on + strand at 3141.037 kb on - strand at 3141.050 kb on + strand at 3141.056 kb on - strand at 3141.056 kb on - strand at 3141.074 kb on - strand at 3141.108 kb on + strand at 3141.109 kb on - strand at 3141.109 kb on - strand at 3141.109 kb on - strand at 3141.109 kb on - strand at 3141.130 kb on + strand at 3141.183 kb on + strand at 3141.183 kb on + strand at 3141.193 kb on + strand at 3141.198 kb on + strand at 3141.199 kb on - strand at 3141.202 kb on + strand at 3141.202 kb on + strand at 3141.202 kb on + strand at 3141.203 kb on - strand at 3141.203 kb on - strand at 3141.229 kb on + strand at 3141.229 kb on + strand at 3141.246 kb on + strand at 3141.247 kb on - strand at 3141.247 kb on - strand at 3141.280 kb on - strand at 3141.282 kb on + strand at 3141.282 kb on + strand at 3141.288 kb on - strand at 3141.288 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 3,139,052 + BT2513 0.73 -2.7 3,139,100 + BT2513 0.77 -2.8 3,139,100 + BT2513 0.77 -2.1 3,139,113 + BT2513 0.78 -2.7 3,139,135 + BT2513 0.79 -1.6 3,139,135 + BT2513 0.79 +1.8 3,139,136 - BT2513 0.79 -0.8 3,139,165 - BT2513 0.81 +2.1 3,139,170 - BT2513 0.82 +0.1 3,139,190 - BT2513 0.83 +2.3 3,139,190 - BT2513 0.83 -2.0 3,139,216 - BT2513 0.85 +1.0 3,139,251 - BT2513 0.87 +0.1 3,139,264 - BT2513 0.88 -1.4 3,139,359 + +0.1 3,139,359 + -1.0 3,139,415 + +2.6 3,139,416 - -2.6 3,139,431 + -0.9 3,139,446 + -2.7 3,139,544 + BT2514 0.34 +0.1 3,139,581 - BT2514 0.53 -2.5 3,139,613 + BT2514 0.69 +2.6 3,139,613 + BT2514 0.69 +1.5 3,139,614 - BT2514 0.69 +0.8 3,139,614 - BT2514 0.69 +2.3 3,139,614 - BT2514 0.69 +2.0 3,139,614 - BT2514 0.69 -2.4 3,139,660 + -0.9 3,139,661 - -1.3 3,139,670 + -1.7 3,139,704 - +2.4 3,139,722 - +1.1 3,139,724 - +1.8 3,139,728 - +0.1 3,139,738 - -0.9 3,139,746 + -0.7 3,139,747 - +0.0 3,139,771 + -2.9 3,139,772 - -1.4 3,139,824 + +0.1 3,139,824 + -1.2 3,139,836 - BT2515 0.15 -0.1 3,139,838 - BT2515 0.16 -1.7 3,139,839 + BT2515 0.17 -2.2 3,139,867 + BT2515 0.35 +1.1 3,139,890 - BT2515 0.51 -1.0 3,139,900 + BT2515 0.57 -0.8 3,139,900 + BT2515 0.57 +2.3 3,140,032 - -0.9 3,140,092 - +0.1 3,140,103 + BT2516 0.10 -4.1 3,140,103 + BT2516 0.10 -3.2 3,140,125 + BT2516 0.13 -0.2 3,140,126 - BT2516 0.14 -2.1 3,140,126 - BT2516 0.14 -1.9 3,140,214 - BT2516 0.26 -3.4 3,140,217 + BT2516 0.27 -0.9 3,140,230 - BT2516 0.29 -1.3 3,140,265 - BT2516 0.34 +2.1 3,140,312 + BT2516 0.40 -0.9 3,140,325 + BT2516 0.42 -4.3 3,140,412 + BT2516 0.55 -1.0 3,140,415 - BT2516 0.55 +1.1 3,140,418 - BT2516 0.56 -3.0 3,140,430 - BT2516 0.57 +1.1 3,140,448 - BT2516 0.60 +0.5 3,140,477 + BT2516 0.64 +1.4 3,140,478 - BT2516 0.64 -1.2 3,140,478 - BT2516 0.64 +0.9 3,140,543 + BT2516 0.74 -3.8 3,140,544 - BT2516 0.74 -2.9 3,140,622 - BT2516 0.85 +2.1 3,140,622 - BT2516 0.85 +0.7 3,140,622 - BT2516 0.85 -2.5 3,140,722 + +0.7 3,140,731 + +2.8 3,140,794 - -2.2 3,140,803 - -1.6 3,140,803 - -1.9 3,140,826 + +0.1 3,140,827 - +3.4 3,140,849 - +2.9 3,140,849 - -2.2 3,140,852 - -0.9 3,140,892 + +2.1 3,140,912 - -2.2 3,140,938 - -3.7 3,140,972 + -0.9 3,140,979 + -1.9 3,140,979 + -1.3 3,140,981 + -0.2 3,140,981 + -2.7 3,140,982 - -3.0 3,140,983 + +1.7 3,140,983 + +4.2 3,140,983 + -4.0 3,140,984 - +1.1 3,140,984 - -3.5 3,140,989 - +0.9 3,140,994 - +0.1 3,141,008 - -0.9 3,141,019 + -1.7 3,141,019 + +0.1 3,141,019 + -0.3 3,141,036 + +0.1 3,141,037 - -0.4 3,141,050 + -2.0 3,141,056 - -0.9 3,141,056 - +1.7 3,141,074 - -0.3 3,141,108 + -2.2 3,141,109 - +0.9 3,141,109 - -2.2 3,141,109 - +0.1 3,141,109 - -0.9 3,141,130 + -0.2 3,141,183 + -1.5 3,141,183 + -2.1 3,141,193 + +0.1 3,141,198 + +2.0 3,141,199 - -1.6 3,141,202 + -1.7 3,141,202 + -1.2 3,141,202 + -1.3 3,141,203 - -0.9 3,141,203 - -0.6 3,141,229 + +1.7 3,141,229 + +0.1 3,141,246 + -0.7 3,141,247 - -2.2 3,141,247 - +0.6 3,141,280 - -1.9 3,141,282 + +1.6 3,141,282 + +2.1 3,141,288 - -2.6 3,141,288 - -4.0
Or see this region's nucleotide sequence