Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2186 and BT2187 are separated by 13 nucleotides BT2187 and BT2188 are separated by 74 nucleotides BT2188 and BT2189 are separated by 23 nucleotides
BT2186: BT2186 - 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) (from data) , at 2,743,993 to 2,745,225
BT2186
BT2187: BT2187 - putative cAMP-binding domain-containing regulatory protein (NCBI ptt file), at 2,745,239 to 2,745,808
BT2187
BT2188: BT2188 - putative oxidoreductase (NCBI ptt file), at 2,745,883 to 2,746,599
BT2188
BT2189: BT2189 - aminopeptidase (NCBI ptt file), at 2,746,623 to 2,747,645
BT2189
Position (kb)
2745
2746 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2745.236 kb on - strand at 2745.236 kb on - strand at 2745.236 kb on - strand at 2745.258 kb on - strand at 2745.288 kb on + strand at 2745.338 kb on + strand, within BT2187 at 2745.365 kb on - strand, within BT2187 at 2745.445 kb on + strand, within BT2187 at 2745.446 kb on - strand, within BT2187 at 2745.512 kb on - strand, within BT2187 at 2745.532 kb on + strand, within BT2187 at 2745.533 kb on - strand, within BT2187 at 2745.539 kb on + strand at 2745.540 kb on - strand, within BT2187 at 2745.636 kb on + strand, within BT2187 at 2745.638 kb on + strand, within BT2187 at 2745.659 kb on + strand, within BT2187 at 2745.659 kb on + strand, within BT2187 at 2745.660 kb on - strand, within BT2187 at 2745.692 kb on - strand, within BT2187 at 2745.694 kb on + strand, within BT2187 at 2745.695 kb on - strand, within BT2187 at 2745.733 kb on - strand, within BT2187 at 2745.750 kb on - strand, within BT2187 at 2745.868 kb on - strand at 2745.868 kb on - strand at 2745.873 kb on - strand at 2745.896 kb on + strand at 2745.897 kb on - strand at 2745.897 kb on - strand at 2745.943 kb on + strand at 2745.943 kb on + strand at 2745.943 kb on + strand at 2745.944 kb on - strand at 2745.944 kb on - strand at 2745.993 kb on - strand, within BT2188 at 2746.029 kb on - strand, within BT2188 at 2746.034 kb on - strand, within BT2188 at 2746.123 kb on - strand, within BT2188 at 2746.162 kb on + strand, within BT2188 at 2746.196 kb on + strand, within BT2188 at 2746.305 kb on - strand, within BT2188 at 2746.315 kb on + strand, within BT2188 at 2746.315 kb on + strand, within BT2188 at 2746.315 kb on + strand, within BT2188 at 2746.316 kb on - strand, within BT2188 at 2746.317 kb on + strand, within BT2188 at 2746.318 kb on - strand, within BT2188 at 2746.345 kb on + strand, within BT2188 at 2746.380 kb on - strand, within BT2188 at 2746.474 kb on - strand, within BT2188 at 2746.624 kb on + strand at 2746.655 kb on + strand at 2746.658 kb on + strand at 2746.686 kb on + strand at 2746.751 kb on + strand, within BT2189 at 2746.764 kb on - strand, within BT2189 at 2746.772 kb on + strand, within BT2189
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 2,745,236 - -0.7 2,745,236 - -2.4 2,745,236 - +2.0 2,745,258 - -1.5 2,745,288 + -0.7 2,745,338 + BT2187 0.17 +1.0 2,745,365 - BT2187 0.22 +1.0 2,745,445 + BT2187 0.36 +1.3 2,745,446 - BT2187 0.36 +2.4 2,745,512 - BT2187 0.48 +2.6 2,745,532 + BT2187 0.51 +0.7 2,745,533 - BT2187 0.52 -0.8 2,745,539 + -0.6 2,745,540 - BT2187 0.53 +1.3 2,745,636 + BT2187 0.70 +2.0 2,745,638 + BT2187 0.70 -0.9 2,745,659 + BT2187 0.74 -2.2 2,745,659 + BT2187 0.74 -0.5 2,745,660 - BT2187 0.74 -0.6 2,745,692 - BT2187 0.79 -0.0 2,745,694 + BT2187 0.80 -0.0 2,745,695 - BT2187 0.80 +2.8 2,745,733 - BT2187 0.87 -1.3 2,745,750 - BT2187 0.90 +1.4 2,745,868 - +3.3 2,745,868 - -3.3 2,745,873 - +2.3 2,745,896 + +0.4 2,745,897 - -2.7 2,745,897 - -0.7 2,745,943 + +0.4 2,745,943 + -2.3 2,745,943 + +1.6 2,745,944 - -2.0 2,745,944 - -0.4 2,745,993 - BT2188 0.15 +1.2 2,746,029 - BT2188 0.20 +3.2 2,746,034 - BT2188 0.21 +0.0 2,746,123 - BT2188 0.33 -1.5 2,746,162 + BT2188 0.39 +1.2 2,746,196 + BT2188 0.44 -1.2 2,746,305 - BT2188 0.59 -1.0 2,746,315 + BT2188 0.60 -0.8 2,746,315 + BT2188 0.60 -3.3 2,746,315 + BT2188 0.60 -2.8 2,746,316 - BT2188 0.60 -2.6 2,746,317 + BT2188 0.61 -1.1 2,746,318 - BT2188 0.61 -0.9 2,746,345 + BT2188 0.64 -0.1 2,746,380 - BT2188 0.69 +0.3 2,746,474 - BT2188 0.82 -2.0 2,746,624 + +1.6 2,746,655 + -2.3 2,746,658 + +2.7 2,746,686 + -3.3 2,746,751 + BT2189 0.13 -0.0 2,746,764 - BT2189 0.14 +1.2 2,746,772 + BT2189 0.15 -0.1
Or see this region's nucleotide sequence