Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT1915

Experiment: BHIS with Chlorpromazine 0.1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT1914 and BT1915 are separated by 532 nucleotidesBT1915 and BT1916 are separated by 20 nucleotidesBT1916 and BT1917 are separated by 20 nucleotides BT1914: BT1914 - thioredoxin-like protein, putative thioredoxin (NCBI ptt file), at 2,403,142 to 2,403,465 BT1914 BT1915: BT1915 - pyruvate carboxylase subunit A (NCBI ptt file), at 2,403,998 to 2,405,509 BT1915 BT1916: BT1916 - conserved hypothetical protein (NCBI ptt file), at 2,405,530 to 2,406,039 BT1916 BT1917: BT1917 - propionyl-CoA carboxylase beta chain (NCBI ptt file), at 2,406,060 to 2,407,595 BT1917 Position (kb) 2403 2404 2405 2406Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2403.005 kb on + strandat 2403.129 kb on + strandat 2403.261 kb on - strand, within BT1914at 2403.261 kb on - strand, within BT1914at 2403.296 kb on - strand, within BT1914at 2403.302 kb on - strand, within BT1914at 2403.302 kb on - strand, within BT1914at 2403.302 kb on - strand, within BT1914at 2403.485 kb on - strandat 2403.506 kb on + strandat 2403.532 kb on - strandat 2403.651 kb on + strandat 2403.758 kb on - strandat 2403.758 kb on - strandat 2403.815 kb on - strandat 2403.920 kb on + strandat 2403.973 kb on - strandat 2403.973 kb on - strandat 2403.973 kb on - strandat 2403.984 kb on + strandat 2403.992 kb on + strandat 2404.075 kb on - strandat 2404.228 kb on + strand, within BT1915at 2404.477 kb on + strand, within BT1915at 2404.498 kb on + strand, within BT1915at 2404.540 kb on + strand, within BT1915at 2404.800 kb on - strand, within BT1915at 2404.834 kb on + strand, within BT1915at 2404.835 kb on - strand, within BT1915at 2404.882 kb on - strand, within BT1915at 2405.109 kb on - strand, within BT1915at 2405.138 kb on + strand, within BT1915at 2405.170 kb on - strand, within BT1915at 2405.190 kb on - strand, within BT1915at 2405.215 kb on - strand, within BT1915at 2405.317 kb on + strand, within BT1915at 2405.408 kb on + strandat 2405.496 kb on + strandat 2405.543 kb on + strandat 2405.602 kb on - strand, within BT1916at 2405.602 kb on - strand, within BT1916at 2405.602 kb on - strand, within BT1916at 2405.722 kb on + strand, within BT1916at 2405.729 kb on + strand, within BT1916at 2405.730 kb on - strand, within BT1916at 2405.730 kb on - strand, within BT1916at 2405.730 kb on - strand, within BT1916at 2405.733 kb on + strand, within BT1916at 2405.734 kb on - strand, within BT1916at 2405.740 kb on + strand, within BT1916at 2405.741 kb on - strand, within BT1916at 2406.015 kb on - strandat 2406.033 kb on + strandat 2406.034 kb on - strandat 2406.036 kb on - strandat 2406.036 kb on - strandat 2406.036 kb on - strandat 2406.038 kb on - strandat 2406.040 kb on + strandat 2406.075 kb on - strandat 2406.252 kb on + strand, within BT1917at 2406.253 kb on - strand, within BT1917at 2406.326 kb on - strand, within BT1917

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM
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2,403,005 + -1.6
2,403,129 + -1.0
2,403,261 - BT1914 0.37 -2.8
2,403,261 - BT1914 0.37 -0.6
2,403,296 - BT1914 0.48 +1.6
2,403,302 - BT1914 0.49 -3.2
2,403,302 - BT1914 0.49 -1.9
2,403,302 - BT1914 0.49 -1.7
2,403,485 - +1.4
2,403,506 + -1.3
2,403,532 - +1.2
2,403,651 + +2.1
2,403,758 - -0.7
2,403,758 - -3.0
2,403,815 - +2.3
2,403,920 + +0.0
2,403,973 - -2.3
2,403,973 - -1.0
2,403,973 - -1.0
2,403,984 + -1.6
2,403,992 + -1.0
2,404,075 - -1.6
2,404,228 + BT1915 0.15 -2.3
2,404,477 + BT1915 0.32 -1.0
2,404,498 + BT1915 0.33 -2.6
2,404,540 + BT1915 0.36 -2.1
2,404,800 - BT1915 0.53 -2.3
2,404,834 + BT1915 0.55 -1.0
2,404,835 - BT1915 0.55 -0.6
2,404,882 - BT1915 0.58 +0.0
2,405,109 - BT1915 0.73 -2.6
2,405,138 + BT1915 0.75 -2.6
2,405,170 - BT1915 0.78 +0.7
2,405,190 - BT1915 0.79 -1.0
2,405,215 - BT1915 0.80 -2.6
2,405,317 + BT1915 0.87 -2.6
2,405,408 + +0.3
2,405,496 + +0.8
2,405,543 + -2.3
2,405,602 - BT1916 0.14 -3.1
2,405,602 - BT1916 0.14 -2.0
2,405,602 - BT1916 0.14 +1.2
2,405,722 + BT1916 0.38 -3.8
2,405,729 + BT1916 0.39 +0.0
2,405,730 - BT1916 0.39 -1.6
2,405,730 - BT1916 0.39 -3.9
2,405,730 - BT1916 0.39 -1.6
2,405,733 + BT1916 0.40 -3.8
2,405,734 - BT1916 0.40 -1.7
2,405,740 + BT1916 0.41 -1.6
2,405,741 - BT1916 0.41 -1.3
2,406,015 - +1.3
2,406,033 + -2.0
2,406,034 - -3.7
2,406,036 - -0.6
2,406,036 - -1.0
2,406,036 - -1.8
2,406,038 - -1.6
2,406,040 + +1.6
2,406,075 - -2.1
2,406,252 + BT1917 0.12 +0.0
2,406,253 - BT1917 0.13 -3.6
2,406,326 - BT1917 0.17 -2.2

Or see this region's nucleotide sequence