Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1914 and BT1915 are separated by 532 nucleotides BT1915 and BT1916 are separated by 20 nucleotides BT1916 and BT1917 are separated by 20 nucleotides
BT1914: BT1914 - thioredoxin-like protein, putative thioredoxin (NCBI ptt file), at 2,403,142 to 2,403,465
BT1914
BT1915: BT1915 - pyruvate carboxylase subunit A (NCBI ptt file), at 2,403,998 to 2,405,509
BT1915
BT1916: BT1916 - conserved hypothetical protein (NCBI ptt file), at 2,405,530 to 2,406,039
BT1916
BT1917: BT1917 - propionyl-CoA carboxylase beta chain (NCBI ptt file), at 2,406,060 to 2,407,595
BT1917
Position (kb)
2403
2404
2405
2406 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2403.005 kb on + strand at 2403.129 kb on + strand at 2403.261 kb on - strand, within BT1914 at 2403.261 kb on - strand, within BT1914 at 2403.296 kb on - strand, within BT1914 at 2403.302 kb on - strand, within BT1914 at 2403.302 kb on - strand, within BT1914 at 2403.302 kb on - strand, within BT1914 at 2403.485 kb on - strand at 2403.506 kb on + strand at 2403.532 kb on - strand at 2403.651 kb on + strand at 2403.758 kb on - strand at 2403.758 kb on - strand at 2403.815 kb on - strand at 2403.920 kb on + strand at 2403.973 kb on - strand at 2403.973 kb on - strand at 2403.973 kb on - strand at 2403.984 kb on + strand at 2403.992 kb on + strand at 2404.075 kb on - strand at 2404.228 kb on + strand, within BT1915 at 2404.477 kb on + strand, within BT1915 at 2404.498 kb on + strand, within BT1915 at 2404.540 kb on + strand, within BT1915 at 2404.800 kb on - strand, within BT1915 at 2404.834 kb on + strand, within BT1915 at 2404.835 kb on - strand, within BT1915 at 2404.882 kb on - strand, within BT1915 at 2405.109 kb on - strand, within BT1915 at 2405.138 kb on + strand, within BT1915 at 2405.170 kb on - strand, within BT1915 at 2405.190 kb on - strand, within BT1915 at 2405.215 kb on - strand, within BT1915 at 2405.317 kb on + strand, within BT1915 at 2405.408 kb on + strand at 2405.496 kb on + strand at 2405.543 kb on + strand at 2405.602 kb on - strand, within BT1916 at 2405.602 kb on - strand, within BT1916 at 2405.602 kb on - strand, within BT1916 at 2405.722 kb on + strand, within BT1916 at 2405.729 kb on + strand, within BT1916 at 2405.730 kb on - strand, within BT1916 at 2405.730 kb on - strand, within BT1916 at 2405.730 kb on - strand, within BT1916 at 2405.733 kb on + strand, within BT1916 at 2405.734 kb on - strand, within BT1916 at 2405.740 kb on + strand, within BT1916 at 2405.741 kb on - strand, within BT1916 at 2406.015 kb on - strand at 2406.033 kb on + strand at 2406.034 kb on - strand at 2406.036 kb on - strand at 2406.036 kb on - strand at 2406.036 kb on - strand at 2406.038 kb on - strand at 2406.040 kb on + strand at 2406.075 kb on - strand at 2406.252 kb on + strand, within BT1917 at 2406.253 kb on - strand, within BT1917 at 2406.326 kb on - strand, within BT1917
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 2,403,005 + -1.6 2,403,129 + -1.0 2,403,261 - BT1914 0.37 -2.8 2,403,261 - BT1914 0.37 -0.6 2,403,296 - BT1914 0.48 +1.6 2,403,302 - BT1914 0.49 -3.2 2,403,302 - BT1914 0.49 -1.9 2,403,302 - BT1914 0.49 -1.7 2,403,485 - +1.4 2,403,506 + -1.3 2,403,532 - +1.2 2,403,651 + +2.1 2,403,758 - -0.7 2,403,758 - -3.0 2,403,815 - +2.3 2,403,920 + +0.0 2,403,973 - -2.3 2,403,973 - -1.0 2,403,973 - -1.0 2,403,984 + -1.6 2,403,992 + -1.0 2,404,075 - -1.6 2,404,228 + BT1915 0.15 -2.3 2,404,477 + BT1915 0.32 -1.0 2,404,498 + BT1915 0.33 -2.6 2,404,540 + BT1915 0.36 -2.1 2,404,800 - BT1915 0.53 -2.3 2,404,834 + BT1915 0.55 -1.0 2,404,835 - BT1915 0.55 -0.6 2,404,882 - BT1915 0.58 +0.0 2,405,109 - BT1915 0.73 -2.6 2,405,138 + BT1915 0.75 -2.6 2,405,170 - BT1915 0.78 +0.7 2,405,190 - BT1915 0.79 -1.0 2,405,215 - BT1915 0.80 -2.6 2,405,317 + BT1915 0.87 -2.6 2,405,408 + +0.3 2,405,496 + +0.8 2,405,543 + -2.3 2,405,602 - BT1916 0.14 -3.1 2,405,602 - BT1916 0.14 -2.0 2,405,602 - BT1916 0.14 +1.2 2,405,722 + BT1916 0.38 -3.8 2,405,729 + BT1916 0.39 +0.0 2,405,730 - BT1916 0.39 -1.6 2,405,730 - BT1916 0.39 -3.9 2,405,730 - BT1916 0.39 -1.6 2,405,733 + BT1916 0.40 -3.8 2,405,734 - BT1916 0.40 -1.7 2,405,740 + BT1916 0.41 -1.6 2,405,741 - BT1916 0.41 -1.3 2,406,015 - +1.3 2,406,033 + -2.0 2,406,034 - -3.7 2,406,036 - -0.6 2,406,036 - -1.0 2,406,036 - -1.8 2,406,038 - -1.6 2,406,040 + +1.6 2,406,075 - -2.1 2,406,252 + BT1917 0.12 +0.0 2,406,253 - BT1917 0.13 -3.6 2,406,326 - BT1917 0.17 -2.2
Or see this region's nucleotide sequence