Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1771 and BT1772 are separated by 2 nucleotides BT1772 and BT1773 are separated by 21 nucleotides
BT1771: BT1771 - putative cell surface protein (NCBI ptt file), at 2,198,734 to 2,200,881
BT1771
BT1772: BT1772 - conserved hypothetical protein (NCBI ptt file), at 2,200,884 to 2,202,164
BT1772
BT1773: BT1773 - hypothetical protein (NCBI ptt file), at 2,202,186 to 2,204,192
BT1773
Position (kb)
2200
2201
2202
2203 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2199.991 kb on + strand, within BT1771 at 2199.991 kb on + strand, within BT1771 at 2199.992 kb on - strand, within BT1771 at 2199.992 kb on - strand, within BT1771 at 2199.992 kb on - strand, within BT1771 at 2199.992 kb on - strand, within BT1771 at 2199.993 kb on + strand, within BT1771 at 2200.029 kb on + strand, within BT1771 at 2200.035 kb on + strand, within BT1771 at 2200.036 kb on - strand, within BT1771 at 2200.036 kb on - strand, within BT1771 at 2200.065 kb on + strand, within BT1771 at 2200.077 kb on + strand, within BT1771 at 2200.177 kb on - strand, within BT1771 at 2200.192 kb on + strand, within BT1771 at 2200.193 kb on - strand, within BT1771 at 2200.193 kb on - strand, within BT1771 at 2200.210 kb on - strand, within BT1771 at 2200.413 kb on + strand, within BT1771 at 2200.571 kb on - strand, within BT1771 at 2200.571 kb on - strand, within BT1771 at 2200.629 kb on + strand, within BT1771 at 2200.885 kb on + strand at 2200.914 kb on - strand at 2201.045 kb on + strand, within BT1772 at 2201.045 kb on + strand, within BT1772 at 2201.046 kb on - strand, within BT1772 at 2201.055 kb on - strand, within BT1772 at 2201.095 kb on + strand, within BT1772 at 2201.119 kb on - strand, within BT1772 at 2201.198 kb on + strand, within BT1772 at 2201.258 kb on - strand, within BT1772 at 2201.261 kb on + strand, within BT1772 at 2201.306 kb on + strand, within BT1772 at 2201.307 kb on - strand, within BT1772 at 2201.353 kb on + strand, within BT1772 at 2201.414 kb on + strand, within BT1772 at 2201.414 kb on + strand, within BT1772 at 2201.415 kb on - strand, within BT1772 at 2201.444 kb on - strand, within BT1772 at 2201.498 kb on - strand, within BT1772 at 2201.598 kb on + strand, within BT1772 at 2201.615 kb on + strand, within BT1772 at 2201.616 kb on - strand, within BT1772 at 2201.633 kb on + strand, within BT1772 at 2201.639 kb on + strand, within BT1772 at 2201.645 kb on + strand, within BT1772 at 2201.710 kb on + strand, within BT1772 at 2201.710 kb on + strand, within BT1772 at 2201.729 kb on + strand, within BT1772 at 2201.733 kb on + strand, within BT1772 at 2201.753 kb on + strand, within BT1772 at 2201.754 kb on - strand, within BT1772 at 2201.805 kb on - strand, within BT1772 at 2201.975 kb on + strand, within BT1772 at 2202.180 kb on + strand at 2202.185 kb on - strand at 2202.200 kb on + strand at 2202.235 kb on + strand at 2202.395 kb on + strand, within BT1773 at 2202.488 kb on + strand, within BT1773 at 2202.500 kb on - strand, within BT1773 at 2202.562 kb on + strand, within BT1773 at 2202.562 kb on + strand, within BT1773 at 2202.567 kb on + strand, within BT1773 at 2202.762 kb on - strand, within BT1773 at 2202.776 kb on + strand, within BT1773 at 2202.777 kb on - strand, within BT1773 at 2202.812 kb on + strand, within BT1773 at 2202.815 kb on - strand, within BT1773 at 2202.877 kb on + strand, within BT1773 at 2202.877 kb on + strand, within BT1773 at 2202.982 kb on + strand, within BT1773 at 2202.982 kb on + strand, within BT1773 at 2202.986 kb on - strand, within BT1773 at 2203.000 kb on + strand, within BT1773 at 2203.000 kb on + strand, within BT1773 at 2203.000 kb on + strand, within BT1773 at 2203.050 kb on - strand, within BT1773 at 2203.097 kb on + strand, within BT1773 at 2203.136 kb on + strand, within BT1773
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 2,199,991 + BT1771 0.59 -1.2 2,199,991 + BT1771 0.59 -0.6 2,199,992 - BT1771 0.59 -3.4 2,199,992 - BT1771 0.59 -2.6 2,199,992 - BT1771 0.59 +1.6 2,199,992 - BT1771 0.59 -0.4 2,199,993 + BT1771 0.59 +1.1 2,200,029 + BT1771 0.60 -2.0 2,200,035 + BT1771 0.61 +1.0 2,200,036 - BT1771 0.61 -0.0 2,200,036 - BT1771 0.61 -1.0 2,200,065 + BT1771 0.62 -0.3 2,200,077 + BT1771 0.63 +1.6 2,200,177 - BT1771 0.67 -1.1 2,200,192 + BT1771 0.68 -3.1 2,200,193 - BT1771 0.68 -3.0 2,200,193 - BT1771 0.68 -1.3 2,200,210 - BT1771 0.69 -2.7 2,200,413 + BT1771 0.78 +1.4 2,200,571 - BT1771 0.86 -2.4 2,200,571 - BT1771 0.86 -1.4 2,200,629 + BT1771 0.88 -1.0 2,200,885 + -0.6 2,200,914 - +0.3 2,201,045 + BT1772 0.13 -2.7 2,201,045 + BT1772 0.13 +2.1 2,201,046 - BT1772 0.13 -2.4 2,201,055 - BT1772 0.13 -1.6 2,201,095 + BT1772 0.16 +1.3 2,201,119 - BT1772 0.18 -1.0 2,201,198 + BT1772 0.25 -1.8 2,201,258 - BT1772 0.29 -2.0 2,201,261 + BT1772 0.29 -1.8 2,201,306 + BT1772 0.33 +1.0 2,201,307 - BT1772 0.33 -1.6 2,201,353 + BT1772 0.37 +0.4 2,201,414 + BT1772 0.41 +1.4 2,201,414 + BT1772 0.41 +0.8 2,201,415 - BT1772 0.41 -0.0 2,201,444 - BT1772 0.44 -2.0 2,201,498 - BT1772 0.48 +1.6 2,201,598 + BT1772 0.56 -0.0 2,201,615 + BT1772 0.57 -0.8 2,201,616 - BT1772 0.57 -1.9 2,201,633 + BT1772 0.58 -0.0 2,201,639 + BT1772 0.59 -0.6 2,201,645 + BT1772 0.59 +1.3 2,201,710 + BT1772 0.64 -2.0 2,201,710 + BT1772 0.64 -1.6 2,201,729 + BT1772 0.66 +0.3 2,201,733 + BT1772 0.66 -1.4 2,201,753 + BT1772 0.68 -1.3 2,201,754 - BT1772 0.68 -1.8 2,201,805 - BT1772 0.72 +0.5 2,201,975 + BT1772 0.85 -0.0 2,202,180 + -2.4 2,202,185 - -1.0 2,202,200 + -1.0 2,202,235 + -2.6 2,202,395 + BT1773 0.10 +2.3 2,202,488 + BT1773 0.15 -1.7 2,202,500 - BT1773 0.16 -3.0 2,202,562 + BT1773 0.19 -3.2 2,202,562 + BT1773 0.19 +2.3 2,202,567 + BT1773 0.19 -2.3 2,202,762 - BT1773 0.29 +1.6 2,202,776 + BT1773 0.29 +2.0 2,202,777 - BT1773 0.29 -2.0 2,202,812 + BT1773 0.31 -0.0 2,202,815 - BT1773 0.31 -2.2 2,202,877 + BT1773 0.34 -1.2 2,202,877 + BT1773 0.34 +2.9 2,202,982 + BT1773 0.40 +2.0 2,202,982 + BT1773 0.40 +3.1 2,202,986 - BT1773 0.40 -0.0 2,203,000 + BT1773 0.41 -2.8 2,203,000 + BT1773 0.41 -0.9 2,203,000 + BT1773 0.41 -0.0 2,203,050 - BT1773 0.43 -0.0 2,203,097 + BT1773 0.45 -1.3 2,203,136 + BT1773 0.47 +1.1
Or see this region's nucleotide sequence