Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0940 and BT0941 are separated by 216 nucleotides BT0941 and BT0942 are separated by 416 nucleotides BT0942 and BT0943 are separated by 231 nucleotides
BT0940: BT0940 - hypothetical protein (NCBI ptt file), at 1,149,936 to 1,150,400
BT0940
BT0941: BT0941 - hypothetical protein (NCBI ptt file), at 1,150,617 to 1,150,880
BT0941
BT0942: BT0942 - putative dinitrogenase reductase activating glycohydrolase (NCBI ptt file), at 1,151,297 to 1,152,085
BT0942
BT0943: BT0943 - penicillin-binding protein 2B (PBP-2B) (NCBI ptt file), at 1,152,317 to 1,153,219
BT0943
Position (kb)
1151
1152
1153 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1150.311 kb on + strand, within BT0940 at 1150.419 kb on - strand at 1150.424 kb on - strand at 1150.428 kb on + strand at 1150.451 kb on + strand at 1150.452 kb on - strand at 1150.522 kb on - strand at 1150.537 kb on - strand at 1150.599 kb on + strand at 1150.604 kb on - strand at 1150.627 kb on + strand at 1150.629 kb on + strand at 1150.629 kb on + strand at 1150.630 kb on - strand at 1150.631 kb on + strand at 1150.683 kb on + strand, within BT0941 at 1150.718 kb on + strand, within BT0941 at 1150.724 kb on + strand, within BT0941 at 1150.738 kb on + strand, within BT0941 at 1150.750 kb on - strand, within BT0941 at 1150.762 kb on - strand, within BT0941 at 1150.778 kb on + strand, within BT0941 at 1150.825 kb on - strand, within BT0941 at 1150.846 kb on - strand, within BT0941 at 1150.851 kb on + strand, within BT0941 at 1150.858 kb on + strand at 1150.860 kb on + strand at 1150.860 kb on + strand at 1150.861 kb on - strand at 1150.863 kb on - strand at 1150.873 kb on + strand at 1150.915 kb on - strand at 1150.919 kb on - strand at 1150.919 kb on - strand at 1150.927 kb on + strand at 1150.927 kb on + strand at 1150.931 kb on - strand at 1151.028 kb on + strand at 1151.042 kb on + strand at 1151.043 kb on - strand at 1151.053 kb on + strand at 1151.053 kb on + strand at 1151.091 kb on + strand at 1151.111 kb on - strand at 1151.152 kb on + strand at 1151.154 kb on + strand at 1151.156 kb on + strand at 1151.230 kb on + strand at 1151.239 kb on + strand at 1151.255 kb on - strand at 1151.296 kb on - strand at 1151.298 kb on + strand at 1151.404 kb on - strand, within BT0942 at 1151.414 kb on + strand, within BT0942 at 1151.462 kb on + strand, within BT0942 at 1151.468 kb on - strand, within BT0942 at 1151.526 kb on - strand, within BT0942 at 1151.531 kb on - strand, within BT0942 at 1151.556 kb on + strand, within BT0942 at 1151.567 kb on + strand, within BT0942 at 1151.593 kb on + strand, within BT0942 at 1151.658 kb on + strand, within BT0942 at 1151.675 kb on - strand, within BT0942 at 1151.817 kb on + strand, within BT0942 at 1151.818 kb on - strand, within BT0942 at 1151.866 kb on - strand, within BT0942 at 1151.866 kb on - strand, within BT0942 at 1151.909 kb on + strand, within BT0942 at 1151.960 kb on - strand, within BT0942 at 1151.961 kb on - strand, within BT0942 at 1152.042 kb on - strand at 1152.042 kb on - strand at 1152.047 kb on - strand at 1152.071 kb on + strand at 1152.090 kb on - strand at 1152.170 kb on + strand at 1152.214 kb on - strand at 1152.285 kb on - strand at 1152.392 kb on + strand at 1152.406 kb on + strand at 1152.407 kb on - strand at 1152.421 kb on + strand, within BT0943 at 1152.422 kb on - strand, within BT0943 at 1152.431 kb on - strand, within BT0943 at 1152.437 kb on - strand, within BT0943 at 1152.471 kb on + strand, within BT0943 at 1152.471 kb on - strand, within BT0943 at 1152.472 kb on - strand, within BT0943 at 1152.485 kb on - strand, within BT0943 at 1152.499 kb on + strand, within BT0943 at 1152.511 kb on + strand, within BT0943 at 1152.521 kb on + strand, within BT0943 at 1152.521 kb on + strand, within BT0943 at 1152.533 kb on + strand, within BT0943 at 1152.604 kb on + strand, within BT0943 at 1152.622 kb on + strand, within BT0943 at 1152.644 kb on + strand, within BT0943 at 1152.645 kb on - strand, within BT0943 at 1152.703 kb on + strand, within BT0943 at 1152.714 kb on - strand, within BT0943 at 1152.737 kb on + strand, within BT0943 at 1152.755 kb on - strand, within BT0943 at 1152.761 kb on + strand, within BT0943 at 1152.776 kb on - strand, within BT0943 at 1152.828 kb on - strand, within BT0943 at 1152.841 kb on + strand, within BT0943 at 1152.851 kb on - strand, within BT0943 at 1152.862 kb on + strand, within BT0943 at 1152.862 kb on + strand, within BT0943 at 1152.866 kb on - strand, within BT0943 at 1152.872 kb on + strand, within BT0943 at 1152.916 kb on + strand, within BT0943 at 1152.917 kb on - strand, within BT0943 at 1152.917 kb on - strand, within BT0943 at 1152.989 kb on - strand, within BT0943 at 1153.033 kb on + strand, within BT0943
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 1,150,311 + BT0940 0.81 +1.8 1,150,419 - -1.5 1,150,424 - -1.5 1,150,428 + -0.9 1,150,451 + -2.0 1,150,452 - -0.5 1,150,522 - -1.7 1,150,537 - +0.7 1,150,599 + +1.5 1,150,604 - +1.1 1,150,627 + +2.0 1,150,629 + +1.1 1,150,629 + -2.5 1,150,630 - -1.5 1,150,631 + +0.5 1,150,683 + BT0941 0.25 +0.3 1,150,718 + BT0941 0.38 +0.0 1,150,724 + BT0941 0.41 +1.5 1,150,738 + BT0941 0.46 -0.5 1,150,750 - BT0941 0.50 +0.9 1,150,762 - BT0941 0.55 -2.5 1,150,778 + BT0941 0.61 -2.1 1,150,825 - BT0941 0.79 +1.1 1,150,846 - BT0941 0.87 -1.2 1,150,851 + BT0941 0.89 +3.4 1,150,858 + -1.1 1,150,860 + -2.1 1,150,860 + -0.5 1,150,861 - -0.1 1,150,863 - +1.5 1,150,873 + -0.7 1,150,915 - -1.5 1,150,919 - +1.1 1,150,919 - +1.8 1,150,927 + +3.4 1,150,927 + -1.9 1,150,931 - -1.5 1,151,028 + +1.1 1,151,042 + -1.5 1,151,043 - -3.2 1,151,053 + -1.0 1,151,053 + -0.5 1,151,091 + -1.5 1,151,111 - -0.5 1,151,152 + -0.6 1,151,154 + +1.5 1,151,156 + -2.1 1,151,230 + -3.8 1,151,239 + -2.3 1,151,255 - -1.5 1,151,296 - +0.1 1,151,298 + -2.5 1,151,404 - BT0942 0.14 -1.3 1,151,414 + BT0942 0.15 +1.2 1,151,462 + BT0942 0.21 +0.2 1,151,468 - BT0942 0.22 +0.5 1,151,526 - BT0942 0.29 -0.1 1,151,531 - BT0942 0.30 +0.5 1,151,556 + BT0942 0.33 -1.5 1,151,567 + BT0942 0.34 +2.1 1,151,593 + BT0942 0.38 -0.5 1,151,658 + BT0942 0.46 +1.5 1,151,675 - BT0942 0.48 +1.1 1,151,817 + BT0942 0.66 -0.2 1,151,818 - BT0942 0.66 -2.8 1,151,866 - BT0942 0.72 +0.5 1,151,866 - BT0942 0.72 +1.8 1,151,909 + BT0942 0.78 -1.9 1,151,960 - BT0942 0.84 -0.3 1,151,961 - BT0942 0.84 -2.5 1,152,042 - +1.4 1,152,042 - -1.5 1,152,047 - +0.5 1,152,071 + -1.5 1,152,090 - -1.7 1,152,170 + -1.5 1,152,214 - -0.5 1,152,285 - -2.5 1,152,392 + +0.1 1,152,406 + -0.5 1,152,407 - +0.5 1,152,421 + BT0943 0.12 -1.5 1,152,422 - BT0943 0.12 +0.4 1,152,431 - BT0943 0.13 +1.1 1,152,437 - BT0943 0.13 -0.8 1,152,471 + BT0943 0.17 +0.3 1,152,471 - BT0943 0.17 +0.2 1,152,472 - BT0943 0.17 +0.1 1,152,485 - BT0943 0.19 +0.5 1,152,499 + BT0943 0.20 +1.5 1,152,511 + BT0943 0.21 -3.3 1,152,521 + BT0943 0.23 +1.0 1,152,521 + BT0943 0.23 -0.5 1,152,533 + BT0943 0.24 -1.5 1,152,604 + BT0943 0.32 -1.8 1,152,622 + BT0943 0.34 -0.5 1,152,644 + BT0943 0.36 -2.1 1,152,645 - BT0943 0.36 +0.5 1,152,703 + BT0943 0.43 +1.5 1,152,714 - BT0943 0.44 -0.4 1,152,737 + BT0943 0.47 +3.3 1,152,755 - BT0943 0.49 -0.5 1,152,761 + BT0943 0.49 -0.5 1,152,776 - BT0943 0.51 +1.8 1,152,828 - BT0943 0.57 -1.5 1,152,841 + BT0943 0.58 +1.8 1,152,851 - BT0943 0.59 -0.5 1,152,862 + BT0943 0.60 -2.1 1,152,862 + BT0943 0.60 +0.6 1,152,866 - BT0943 0.61 -3.1 1,152,872 + BT0943 0.61 -1.9 1,152,916 + BT0943 0.66 -2.9 1,152,917 - BT0943 0.66 +1.5 1,152,917 - BT0943 0.66 -1.5 1,152,989 - BT0943 0.74 -0.5 1,153,033 + BT0943 0.79 -1.2
Or see this region's nucleotide sequence