Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0926 and BT0927 are separated by 125 nucleotides BT0927 and BT0928 are separated by 22 nucleotides BT0928 and BT0929 are separated by 132 nucleotides
BT0926: BT0926 - ABC-type transport, permease protein (NCBI ptt file), at 1,139,639 to 1,140,595
BT0926
BT0927: BT0927 - two-component system sensor histidine kinase (NCBI ptt file), at 1,140,721 to 1,141,998
BT0927
BT0928: BT0928 - two-component system response regulator (NCBI ptt file), at 1,142,021 to 1,142,698
BT0928
BT0929: BT0929 - prolyl-tRNA synthetase (NCBI ptt file), at 1,142,831 to 1,144,324
BT0929
Position (kb)
1140
1141
1142 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1139.740 kb on + strand, within BT0926 at 1139.740 kb on + strand, within BT0926 at 1139.746 kb on + strand, within BT0926 at 1139.773 kb on + strand, within BT0926 at 1139.836 kb on + strand, within BT0926 at 1139.989 kb on + strand, within BT0926 at 1139.996 kb on - strand, within BT0926 at 1140.035 kb on - strand, within BT0926 at 1140.180 kb on + strand, within BT0926 at 1140.181 kb on - strand, within BT0926 at 1140.257 kb on + strand, within BT0926 at 1140.258 kb on - strand, within BT0926 at 1140.450 kb on + strand, within BT0926 at 1140.538 kb on + strand at 1140.538 kb on + strand at 1140.558 kb on - strand at 1140.601 kb on + strand at 1140.602 kb on - strand at 1140.621 kb on + strand at 1140.649 kb on + strand at 1140.716 kb on + strand at 1141.523 kb on - strand, within BT0927 at 1141.719 kb on - strand, within BT0927 at 1141.914 kb on + strand at 1142.230 kb on + strand, within BT0928 at 1142.235 kb on + strand, within BT0928 at 1142.297 kb on + strand at 1142.297 kb on + strand, within BT0928 at 1142.406 kb on + strand, within BT0928 at 1142.468 kb on - strand, within BT0928 at 1142.542 kb on + strand, within BT0928 at 1142.571 kb on + strand, within BT0928 at 1142.664 kb on - strand at 1142.721 kb on - strand at 1142.743 kb on - strand at 1142.827 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 1,139,740 + BT0926 0.11 -0.5 1,139,740 + BT0926 0.11 -2.5 1,139,746 + BT0926 0.11 -1.8 1,139,773 + BT0926 0.14 +0.5 1,139,836 + BT0926 0.21 -1.8 1,139,989 + BT0926 0.37 +2.7 1,139,996 - BT0926 0.37 -0.9 1,140,035 - BT0926 0.41 +2.3 1,140,180 + BT0926 0.57 +0.2 1,140,181 - BT0926 0.57 -1.5 1,140,257 + BT0926 0.65 +0.1 1,140,258 - BT0926 0.65 -1.5 1,140,450 + BT0926 0.85 -0.2 1,140,538 + +1.8 1,140,538 + +0.8 1,140,558 - +2.1 1,140,601 + +0.5 1,140,602 - +3.1 1,140,621 + -1.5 1,140,649 + -4.0 1,140,716 + -2.1 1,141,523 - BT0927 0.63 -2.8 1,141,719 - BT0927 0.78 -1.5 1,141,914 + -1.5 1,142,230 + BT0928 0.31 +1.5 1,142,235 + BT0928 0.32 +1.7 1,142,297 + -0.5 1,142,297 + BT0928 0.41 -1.2 1,142,406 + BT0928 0.57 -0.2 1,142,468 - BT0928 0.66 -1.2 1,142,542 + BT0928 0.77 -0.2 1,142,571 + BT0928 0.81 -1.5 1,142,664 - -0.5 1,142,721 - +0.7 1,142,743 - -1.4 1,142,827 - +1.2
Or see this region's nucleotide sequence