Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0671 and BT0672 are separated by 31 nucleotides BT0672 and BT0673 are separated by 90 nucleotides BT0673 and BT0674 are separated by 176 nucleotides
BT0671: BT0671 - outer membrane protein TolC, putative (NCBI ptt file), at 828,717 to 830,216
BT0671
BT0672: BT0672 - drug efflux protein (NCBI ptt file), at 830,248 to 833,493
BT0672
BT0673: BT0673 - NADP(H) oxidoreductase (NCBI ptt file), at 833,584 to 834,138
BT0673
BT0674: BT0674 - carboxynorspermidine decarboxylase (NCBI ptt file), at 834,315 to 835,454
BT0674
Position (kb) 830
831
832
833
834 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 829.565 kb on + strand, within BT0671 at 829.566 kb on - strand, within BT0671 at 830.043 kb on - strand, within BT0671 at 830.234 kb on + strand at 830.259 kb on - strand at 830.402 kb on + strand at 830.502 kb on - strand at 830.534 kb on + strand at 830.631 kb on + strand, within BT0672 at 830.748 kb on + strand, within BT0672 at 830.831 kb on - strand, within BT0672 at 830.923 kb on + strand, within BT0672 at 830.923 kb on + strand, within BT0672 at 830.924 kb on - strand, within BT0672 at 831.097 kb on + strand, within BT0672 at 831.201 kb on + strand, within BT0672 at 831.244 kb on + strand, within BT0672 at 831.252 kb on + strand, within BT0672 at 831.345 kb on - strand, within BT0672 at 831.416 kb on - strand, within BT0672 at 831.440 kb on + strand, within BT0672 at 831.441 kb on - strand, within BT0672 at 831.442 kb on + strand, within BT0672 at 831.457 kb on - strand, within BT0672 at 831.490 kb on - strand, within BT0672 at 831.523 kb on - strand, within BT0672 at 831.823 kb on + strand, within BT0672 at 831.967 kb on + strand, within BT0672 at 831.991 kb on - strand, within BT0672 at 832.078 kb on + strand, within BT0672 at 832.078 kb on + strand, within BT0672 at 832.181 kb on - strand, within BT0672 at 832.245 kb on + strand, within BT0672 at 832.292 kb on - strand, within BT0672 at 832.350 kb on + strand, within BT0672 at 832.351 kb on - strand, within BT0672 at 832.354 kb on + strand, within BT0672 at 832.388 kb on + strand, within BT0672 at 832.449 kb on + strand, within BT0672 at 832.478 kb on + strand, within BT0672 at 832.479 kb on - strand, within BT0672 at 832.620 kb on + strand, within BT0672 at 832.633 kb on + strand, within BT0672 at 832.724 kb on + strand, within BT0672 at 832.724 kb on + strand, within BT0672 at 832.858 kb on + strand, within BT0672 at 832.865 kb on - strand, within BT0672 at 832.866 kb on + strand, within BT0672 at 832.943 kb on - strand, within BT0672 at 832.945 kb on - strand, within BT0672 at 833.025 kb on + strand, within BT0672 at 833.040 kb on + strand, within BT0672 at 833.040 kb on + strand, within BT0672 at 833.292 kb on + strand at 833.397 kb on + strand at 833.398 kb on - strand at 833.415 kb on - strand at 833.509 kb on - strand at 833.516 kb on + strand at 833.517 kb on - strand at 833.553 kb on + strand at 833.557 kb on + strand at 833.571 kb on + strand at 833.573 kb on + strand at 833.580 kb on + strand at 833.581 kb on - strand at 833.640 kb on - strand, within BT0673 at 833.798 kb on + strand, within BT0673 at 833.799 kb on - strand, within BT0673 at 833.828 kb on - strand, within BT0673 at 833.943 kb on - strand, within BT0673 at 834.154 kb on + strand at 834.348 kb on + strand at 834.353 kb on + strand at 834.354 kb on - strand at 834.354 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 829,565 + BT0671 0.57 +1.7 829,566 - BT0671 0.57 +1.5 830,043 - BT0671 0.88 +1.7 830,234 + -1.3 830,259 - -0.3 830,402 + -0.0 830,502 - +1.3 830,534 + -3.8 830,631 + BT0672 0.12 -1.3 830,748 + BT0672 0.15 -1.1 830,831 - BT0672 0.18 -3.3 830,923 + BT0672 0.21 -1.7 830,923 + BT0672 0.21 -3.8 830,924 - BT0672 0.21 -1.3 831,097 + BT0672 0.26 -0.3 831,201 + BT0672 0.29 -1.7 831,244 + BT0672 0.31 -2.1 831,252 + BT0672 0.31 -2.3 831,345 - BT0672 0.34 -1.3 831,416 - BT0672 0.36 +1.5 831,440 + BT0672 0.37 -4.0 831,441 - BT0672 0.37 +0.4 831,442 + BT0672 0.37 -1.3 831,457 - BT0672 0.37 -1.1 831,490 - BT0672 0.38 -1.3 831,523 - BT0672 0.39 -3.1 831,823 + BT0672 0.49 -0.7 831,967 + BT0672 0.53 +0.3 831,991 - BT0672 0.54 -3.5 832,078 + BT0672 0.56 -0.3 832,078 + BT0672 0.56 -1.0 832,181 - BT0672 0.60 +2.0 832,245 + BT0672 0.62 -1.9 832,292 - BT0672 0.63 +1.3 832,350 + BT0672 0.65 -0.3 832,351 - BT0672 0.65 -1.0 832,354 + BT0672 0.65 -2.7 832,388 + BT0672 0.66 +0.7 832,449 + BT0672 0.68 -1.1 832,478 + BT0672 0.69 +1.4 832,479 - BT0672 0.69 -1.3 832,620 + BT0672 0.73 -0.3 832,633 + BT0672 0.73 +1.7 832,724 + BT0672 0.76 +0.8 832,724 + BT0672 0.76 -2.7 832,858 + BT0672 0.80 -1.6 832,865 - BT0672 0.81 -1.2 832,866 + BT0672 0.81 +0.4 832,943 - BT0672 0.83 -1.1 832,945 - BT0672 0.83 +0.3 833,025 + BT0672 0.86 -3.5 833,040 + BT0672 0.86 -0.2 833,040 + BT0672 0.86 -0.3 833,292 + -2.7 833,397 + -1.9 833,398 - -1.7 833,415 - +0.1 833,509 - +0.6 833,516 + -1.1 833,517 - -4.1 833,553 + +2.1 833,557 + -0.3 833,571 + +0.3 833,573 + -0.3 833,580 + +1.3 833,581 - +0.8 833,640 - BT0673 0.10 -1.5 833,798 + BT0673 0.39 -1.2 833,799 - BT0673 0.39 +1.4 833,828 - BT0673 0.44 +0.7 833,943 - BT0673 0.65 -0.5 834,154 + +0.7 834,348 + +1.3 834,353 + +3.1 834,354 - +1.5 834,354 - +0.0
Or see this region's nucleotide sequence