Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0670 and BT0671 overlap by 4 nucleotides BT0671 and BT0672 are separated by 31 nucleotides
BT0670: BT0670 - putative cation efflux system (NCBI ptt file), at 825,613 to 828,720
BT0670
BT0671: BT0671 - outer membrane protein TolC, putative (NCBI ptt file), at 828,717 to 830,216
BT0671
BT0672: BT0672 - drug efflux protein (NCBI ptt file), at 830,248 to 833,493
BT0672
Position (kb) 828
829
830
831 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 828.062 kb on - strand, within BT0670 at 828.345 kb on + strand, within BT0670 at 828.396 kb on - strand, within BT0670 at 828.663 kb on + strand at 828.664 kb on - strand at 828.778 kb on + strand at 828.781 kb on - strand at 828.871 kb on - strand, within BT0671 at 829.021 kb on - strand, within BT0671 at 829.073 kb on + strand, within BT0671 at 829.080 kb on + strand, within BT0671 at 829.124 kb on + strand, within BT0671 at 829.565 kb on + strand, within BT0671 at 829.566 kb on - strand, within BT0671 at 830.043 kb on - strand, within BT0671 at 830.234 kb on + strand at 830.259 kb on - strand at 830.402 kb on + strand at 830.502 kb on - strand at 830.534 kb on + strand at 830.631 kb on + strand, within BT0672 at 830.748 kb on + strand, within BT0672 at 830.831 kb on - strand, within BT0672 at 830.923 kb on + strand, within BT0672 at 830.923 kb on + strand, within BT0672 at 830.924 kb on - strand, within BT0672 at 831.097 kb on + strand, within BT0672 at 831.201 kb on + strand, within BT0672
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 828,062 - BT0670 0.79 -0.3 828,345 + BT0670 0.88 -1.3 828,396 - BT0670 0.90 -0.0 828,663 + +0.7 828,664 - -0.5 828,778 + -1.9 828,781 - -1.3 828,871 - BT0671 0.10 -2.7 829,021 - BT0671 0.20 -1.9 829,073 + BT0671 0.24 -2.9 829,080 + BT0671 0.24 +2.8 829,124 + BT0671 0.27 +1.7 829,565 + BT0671 0.57 +1.7 829,566 - BT0671 0.57 +1.5 830,043 - BT0671 0.88 +1.7 830,234 + -1.3 830,259 - -0.3 830,402 + -0.0 830,502 - +1.3 830,534 + -3.8 830,631 + BT0672 0.12 -1.3 830,748 + BT0672 0.15 -1.1 830,831 - BT0672 0.18 -3.3 830,923 + BT0672 0.21 -1.7 830,923 + BT0672 0.21 -3.8 830,924 - BT0672 0.21 -1.3 831,097 + BT0672 0.26 -0.3 831,201 + BT0672 0.29 -1.7
Or see this region's nucleotide sequence