Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT0580

Experiment: BHIS with Chlorpromazine 0.1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT0578 and BT0579 are separated by 42 nucleotidesBT0579 and BT0580 are separated by 75 nucleotidesBT0580 and BT0581 are separated by 90 nucleotidesBT0581 and BT0582 are separated by 113 nucleotides BT0578: BT0578 - excinuclease ABC subunit A (NCBI ptt file), at 714,364 to 717,204 BT0578 BT0579: BT0579 - putative transcription regulator (NCBI ptt file), at 717,247 to 717,726 BT0579 BT0580: BT0580 - putative H+/peptide symporter (NCBI ptt file), at 717,802 to 719,316 BT0580 BT0581: BT0581 - putative acetyltransferase, GNAT family (NCBI ptt file), at 719,407 to 719,904 BT0581 BT0582: BT0582 - conserved hypothetical protein (NCBI ptt file), at 720,018 to 720,398 BT0582 Position (kb) 717 718 719 720Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 716.804 kb on + strand, within BT0578at 716.835 kb on + strand, within BT0578at 716.838 kb on + strand, within BT0578at 716.894 kb on + strand, within BT0578at 716.985 kb on - strandat 717.000 kb on + strandat 717.001 kb on - strandat 717.001 kb on - strandat 717.003 kb on + strandat 717.046 kb on - strandat 717.074 kb on - strandat 717.081 kb on - strandat 717.116 kb on - strandat 717.127 kb on + strandat 717.169 kb on - strandat 717.206 kb on - strandat 717.216 kb on - strandat 717.228 kb on - strandat 717.230 kb on + strandat 717.390 kb on + strand, within BT0579at 717.756 kb on - strandat 717.801 kb on + strandat 717.830 kb on + strandat 717.872 kb on - strandat 717.910 kb on - strandat 717.910 kb on - strandat 717.943 kb on - strandat 717.946 kb on + strandat 717.947 kb on - strandat 717.993 kb on + strand, within BT0580at 717.994 kb on - strand, within BT0580at 718.019 kb on - strand, within BT0580at 718.029 kb on + strand, within BT0580at 718.077 kb on + strand, within BT0580at 718.105 kb on - strand, within BT0580at 718.125 kb on + strand, within BT0580at 718.125 kb on + strand, within BT0580at 718.204 kb on + strand, within BT0580at 718.220 kb on - strand, within BT0580at 718.256 kb on - strand, within BT0580at 718.257 kb on + strand, within BT0580at 718.257 kb on + strand, within BT0580at 718.307 kb on + strand, within BT0580at 718.308 kb on - strand, within BT0580at 718.308 kb on - strand, within BT0580at 718.311 kb on + strand, within BT0580at 718.312 kb on - strand, within BT0580at 718.386 kb on + strand, within BT0580at 718.405 kb on - strand, within BT0580at 718.498 kb on - strand, within BT0580at 718.524 kb on - strand, within BT0580at 718.528 kb on - strand, within BT0580at 718.632 kb on - strand, within BT0580at 718.662 kb on + strand, within BT0580at 718.662 kb on + strand, within BT0580at 718.667 kb on - strand, within BT0580at 718.667 kb on - strand, within BT0580at 718.667 kb on - strand, within BT0580at 718.667 kb on - strand, within BT0580at 718.756 kb on + strand, within BT0580at 718.779 kb on + strand, within BT0580at 718.779 kb on + strand, within BT0580at 718.786 kb on - strand, within BT0580at 718.946 kb on + strand, within BT0580at 719.136 kb on + strand, within BT0580at 719.271 kb on + strandat 719.364 kb on - strandat 719.388 kb on + strandat 719.391 kb on - strandat 719.391 kb on - strandat 719.412 kb on + strandat 719.427 kb on + strandat 719.427 kb on + strandat 719.428 kb on - strandat 719.428 kb on - strandat 719.429 kb on + strandat 719.429 kb on + strandat 719.429 kb on + strandat 719.430 kb on - strandat 719.439 kb on + strandat 719.441 kb on + strandat 719.577 kb on - strand, within BT0581at 719.577 kb on - strand, within BT0581at 719.659 kb on + strand, within BT0581at 719.839 kb on + strand, within BT0581at 719.878 kb on + strandat 719.905 kb on - strandat 719.944 kb on - strandat 719.963 kb on - strandat 720.080 kb on - strand, within BT0582at 720.157 kb on - strand, within BT0582at 720.261 kb on + strand, within BT0582

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM
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716,804 + BT0578 0.86 -2.6
716,835 + BT0578 0.87 -1.9
716,838 + BT0578 0.87 -1.9
716,894 + BT0578 0.89 +0.7
716,985 - -0.3
717,000 + -2.3
717,001 - -0.5
717,001 - -2.1
717,003 + -1.9
717,046 - -1.9
717,074 - -2.3
717,081 - -3.4
717,116 - -2.6
717,127 + -0.7
717,169 - -1.3
717,206 - -0.7
717,216 - -1.9
717,228 - +2.9
717,230 + -1.3
717,390 + BT0579 0.30 +2.3
717,756 - +0.3
717,801 + +2.2
717,830 + +1.3
717,872 - +1.1
717,910 - +3.2
717,910 - +1.3
717,943 - -0.9
717,946 + +0.0
717,947 - +2.4
717,993 + BT0580 0.13 +4.4
717,994 - BT0580 0.13 +1.5
718,019 - BT0580 0.14 +3.0
718,029 + BT0580 0.15 +1.1
718,077 + BT0580 0.18 -1.9
718,105 - BT0580 0.20 +2.6
718,125 + BT0580 0.21 +3.6
718,125 + BT0580 0.21 +1.2
718,204 + BT0580 0.27 +0.6
718,220 - BT0580 0.28 +1.9
718,256 - BT0580 0.30 +1.8
718,257 + BT0580 0.30 +3.1
718,257 + BT0580 0.30 +1.0
718,307 + BT0580 0.33 +4.9
718,308 - BT0580 0.33 +2.1
718,308 - BT0580 0.33 +2.0
718,311 + BT0580 0.34 +3.5
718,312 - BT0580 0.34 +1.7
718,386 + BT0580 0.39 +2.3
718,405 - BT0580 0.40 +2.1
718,498 - BT0580 0.46 +0.9
718,524 - BT0580 0.48 +2.1
718,528 - BT0580 0.48 +2.4
718,632 - BT0580 0.55 -1.3
718,662 + BT0580 0.57 +2.0
718,662 + BT0580 0.57 +2.9
718,667 - BT0580 0.57 +0.9
718,667 - BT0580 0.57 -0.9
718,667 - BT0580 0.57 -1.0
718,667 - BT0580 0.57 -1.3
718,756 + BT0580 0.63 +0.3
718,779 + BT0580 0.64 +2.3
718,779 + BT0580 0.64 +1.9
718,786 - BT0580 0.65 +3.2
718,946 + BT0580 0.76 +0.4
719,136 + BT0580 0.88 +2.6
719,271 + +1.3
719,364 - +4.9
719,388 + +0.4
719,391 - -2.2
719,391 - +0.7
719,412 + +0.1
719,427 + -1.3
719,427 + -0.3
719,428 - +0.5
719,428 - -0.0
719,429 + -2.9
719,429 + -1.8
719,429 + -0.7
719,430 - -1.3
719,439 + +1.7
719,441 + +0.7
719,577 - BT0581 0.34 -1.9
719,577 - BT0581 0.34 +2.6
719,659 + BT0581 0.51 -3.0
719,839 + BT0581 0.87 -0.9
719,878 + -1.9
719,905 - +0.7
719,944 - -0.3
719,963 - +0.7
720,080 - BT0582 0.16 -2.1
720,157 - BT0582 0.36 +0.7
720,261 + BT0582 0.64 -0.3

Or see this region's nucleotide sequence