Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0361 and BT0362 are separated by 25 nucleotides BT0362 and BT0363 are separated by 4 nucleotides
BT0361: BT0361 - hypothetical protein (NCBI ptt file), at 433,972 to 435,777
BT0361
BT0362: BT0362 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 435,803 to 438,952
BT0362
BT0363: BT0363 - putative outer membrane protein (NCBI ptt file), at 438,957 to 440,732
BT0363
Position (kb) 435
436
437
438
439 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 434.876 kb on + strand, within BT0361 at 434.876 kb on + strand, within BT0361 at 434.876 kb on + strand at 434.928 kb on + strand, within BT0361 at 434.929 kb on - strand, within BT0361 at 434.933 kb on + strand, within BT0361 at 434.933 kb on + strand, within BT0361 at 435.050 kb on + strand, within BT0361 at 435.078 kb on - strand, within BT0361 at 435.298 kb on + strand, within BT0361 at 435.384 kb on + strand, within BT0361 at 435.423 kb on + strand, within BT0361 at 435.445 kb on + strand, within BT0361 at 435.491 kb on + strand, within BT0361 at 435.556 kb on - strand, within BT0361 at 435.556 kb on - strand, within BT0361 at 435.758 kb on + strand at 435.758 kb on + strand at 435.860 kb on - strand at 435.861 kb on + strand at 435.952 kb on + strand at 436.053 kb on + strand at 436.545 kb on + strand, within BT0362 at 436.607 kb on - strand, within BT0362 at 436.881 kb on + strand, within BT0362 at 436.881 kb on + strand, within BT0362 at 436.884 kb on - strand, within BT0362 at 436.995 kb on + strand, within BT0362 at 437.109 kb on + strand, within BT0362 at 437.113 kb on + strand, within BT0362 at 437.150 kb on - strand, within BT0362 at 437.165 kb on + strand, within BT0362 at 437.169 kb on + strand, within BT0362 at 437.173 kb on + strand at 437.188 kb on - strand, within BT0362 at 437.282 kb on - strand, within BT0362 at 437.282 kb on - strand, within BT0362 at 437.290 kb on - strand, within BT0362 at 437.325 kb on + strand, within BT0362 at 437.382 kb on + strand, within BT0362 at 437.386 kb on + strand, within BT0362 at 437.473 kb on - strand, within BT0362 at 437.473 kb on - strand, within BT0362 at 437.554 kb on - strand at 437.635 kb on + strand, within BT0362 at 437.668 kb on - strand, within BT0362 at 437.698 kb on + strand, within BT0362 at 437.711 kb on + strand, within BT0362 at 437.712 kb on - strand, within BT0362 at 437.712 kb on - strand, within BT0362 at 437.734 kb on + strand, within BT0362 at 437.826 kb on - strand, within BT0362 at 437.841 kb on + strand, within BT0362 at 437.910 kb on + strand, within BT0362 at 437.914 kb on - strand, within BT0362 at 438.135 kb on + strand, within BT0362 at 438.135 kb on + strand, within BT0362 at 438.142 kb on - strand, within BT0362 at 438.209 kb on - strand, within BT0362 at 438.240 kb on - strand, within BT0362 at 438.273 kb on + strand, within BT0362 at 438.274 kb on - strand, within BT0362 at 438.277 kb on + strand, within BT0362 at 438.308 kb on - strand, within BT0362 at 438.417 kb on + strand, within BT0362 at 438.418 kb on - strand, within BT0362 at 438.418 kb on - strand, within BT0362 at 438.422 kb on - strand, within BT0362 at 438.460 kb on - strand, within BT0362 at 438.460 kb on - strand, within BT0362 at 438.460 kb on - strand, within BT0362 at 438.467 kb on + strand, within BT0362 at 438.467 kb on + strand, within BT0362 at 438.468 kb on - strand, within BT0362 at 438.610 kb on - strand, within BT0362 at 438.696 kb on - strand at 438.958 kb on + strand at 438.999 kb on + strand at 439.065 kb on - strand at 439.115 kb on + strand at 439.155 kb on + strand, within BT0363 at 439.155 kb on + strand, within BT0363 at 439.308 kb on + strand, within BT0363 at 439.351 kb on + strand, within BT0363 at 439.397 kb on - strand, within BT0363 at 439.523 kb on + strand, within BT0363 at 439.549 kb on + strand, within BT0363 at 439.708 kb on + strand, within BT0363 at 439.800 kb on + strand, within BT0363 at 439.868 kb on + strand, within BT0363
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 434,876 + BT0361 0.50 -1.6 434,876 + BT0361 0.50 -0.9 434,876 + -0.3 434,928 + BT0361 0.53 -0.1 434,929 - BT0361 0.53 -1.1 434,933 + BT0361 0.53 +1.2 434,933 + BT0361 0.53 -1.9 435,050 + BT0361 0.60 +1.5 435,078 - BT0361 0.61 +0.2 435,298 + BT0361 0.73 +0.1 435,384 + BT0361 0.78 +1.6 435,423 + BT0361 0.80 -0.5 435,445 + BT0361 0.82 -2.7 435,491 + BT0361 0.84 +0.1 435,556 - BT0361 0.88 -0.5 435,556 - BT0361 0.88 +1.2 435,758 + +3.1 435,758 + -0.3 435,860 - +0.5 435,861 + +1.2 435,952 + -2.1 436,053 + +0.2 436,545 + BT0362 0.24 -3.3 436,607 - BT0362 0.26 -1.9 436,881 + BT0362 0.34 -2.1 436,881 + BT0362 0.34 +0.1 436,884 - BT0362 0.34 -1.8 436,995 + BT0362 0.38 -0.5 437,109 + BT0362 0.41 +2.2 437,113 + BT0362 0.42 -0.1 437,150 - BT0362 0.43 +1.3 437,165 + BT0362 0.43 +0.1 437,169 + BT0362 0.43 +0.8 437,173 + +1.2 437,188 - BT0362 0.44 -1.3 437,282 - BT0362 0.47 -1.2 437,282 - BT0362 0.47 +0.8 437,290 - BT0362 0.47 -0.3 437,325 + BT0362 0.48 +1.2 437,382 + BT0362 0.50 +0.3 437,386 + BT0362 0.50 -2.1 437,473 - BT0362 0.53 -1.7 437,473 - BT0362 0.53 +2.6 437,554 - -1.3 437,635 + BT0362 0.58 -2.3 437,668 - BT0362 0.59 -1.1 437,698 + BT0362 0.60 -2.1 437,711 + BT0362 0.61 -0.3 437,712 - BT0362 0.61 -1.6 437,712 - BT0362 0.61 -1.3 437,734 + BT0362 0.61 +0.7 437,826 - BT0362 0.64 +2.2 437,841 + BT0362 0.65 -0.3 437,910 + BT0362 0.67 -1.6 437,914 - BT0362 0.67 -0.2 438,135 + BT0362 0.74 -0.3 438,135 + BT0362 0.74 +2.7 438,142 - BT0362 0.74 -3.3 438,209 - BT0362 0.76 +1.7 438,240 - BT0362 0.77 +0.4 438,273 + BT0362 0.78 +3.0 438,274 - BT0362 0.78 -0.1 438,277 + BT0362 0.79 +0.7 438,308 - BT0362 0.80 +0.7 438,417 + BT0362 0.83 -2.3 438,418 - BT0362 0.83 +0.7 438,418 - BT0362 0.83 +0.1 438,422 - BT0362 0.83 -0.3 438,460 - BT0362 0.84 +0.2 438,460 - BT0362 0.84 +1.7 438,460 - BT0362 0.84 +0.9 438,467 + BT0362 0.85 -1.3 438,467 + BT0362 0.85 -0.3 438,468 - BT0362 0.85 +2.5 438,610 - BT0362 0.89 +1.2 438,696 - +2.0 438,958 + +0.4 438,999 + +1.3 439,065 - +1.2 439,115 + -1.9 439,155 + BT0363 0.11 +0.7 439,155 + BT0363 0.11 -1.9 439,308 + BT0363 0.20 +0.7 439,351 + BT0363 0.22 +0.8 439,397 - BT0363 0.25 -0.3 439,523 + BT0363 0.32 +1.7 439,549 + BT0363 0.33 +0.7 439,708 + BT0363 0.42 -0.1 439,800 + BT0363 0.47 +0.2 439,868 + BT0363 0.51 -2.3
Or see this region's nucleotide sequence