Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0308 and BT0309 are separated by 74 nucleotides BT0309 and BT0310 overlap by 17 nucleotides BT0310 and BT0311 are separated by 86 nucleotides
BT0308: BT0308 - putative glycosyl hydrolase (NCBI ptt file), at 370,710 to 371,414
BT0308
BT0309: BT0309 - Dihydrolipoamide dehydrogenase (NCBI ptt file), at 371,489 to 372,832
BT0309
BT0310: BT0310 - lipoate-protein ligase A (NCBI ptt file), at 372,816 to 373,535
BT0310
BT0311: BT0311 - lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (NCBI ptt file), at 373,622 to 374,992
BT0311
Position (kb) 371
372
373 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 370.491 kb on + strand at 370.492 kb on - strand at 370.495 kb on + strand at 370.495 kb on + strand at 370.495 kb on + strand at 370.497 kb on + strand at 370.500 kb on + strand at 370.532 kb on + strand at 370.733 kb on - strand at 370.764 kb on - strand at 370.780 kb on - strand at 370.790 kb on + strand, within BT0308 at 370.931 kb on + strand, within BT0308 at 370.940 kb on + strand, within BT0308 at 370.996 kb on - strand, within BT0308 at 371.005 kb on - strand, within BT0308 at 371.006 kb on + strand, within BT0308 at 371.052 kb on - strand, within BT0308 at 371.161 kb on + strand, within BT0308 at 371.161 kb on + strand, within BT0308 at 371.162 kb on - strand, within BT0308 at 371.193 kb on + strand, within BT0308 at 371.193 kb on + strand, within BT0308 at 371.272 kb on - strand, within BT0308 at 371.298 kb on - strand, within BT0308 at 371.318 kb on + strand, within BT0308 at 371.432 kb on - strand at 371.489 kb on - strand at 371.506 kb on + strand at 371.507 kb on - strand at 371.507 kb on - strand at 371.595 kb on + strand at 371.623 kb on + strand at 371.623 kb on + strand at 371.643 kb on + strand, within BT0309 at 371.796 kb on - strand, within BT0309 at 371.954 kb on - strand, within BT0309 at 372.013 kb on + strand, within BT0309 at 372.015 kb on + strand, within BT0309 at 372.015 kb on + strand, within BT0309 at 372.143 kb on - strand, within BT0309 at 372.220 kb on - strand, within BT0309 at 372.242 kb on - strand, within BT0309 at 372.290 kb on - strand, within BT0309 at 372.322 kb on - strand, within BT0309 at 372.408 kb on - strand, within BT0309 at 372.488 kb on + strand, within BT0309 at 372.489 kb on - strand, within BT0309 at 372.541 kb on + strand, within BT0309 at 372.542 kb on - strand, within BT0309 at 372.586 kb on + strand, within BT0309 at 372.649 kb on - strand, within BT0309 at 372.661 kb on - strand, within BT0309 at 372.694 kb on + strand, within BT0309 at 372.695 kb on - strand, within BT0309 at 372.695 kb on - strand, within BT0309 at 372.739 kb on - strand at 372.797 kb on - strand at 372.932 kb on - strand, within BT0310 at 372.932 kb on - strand, within BT0310 at 372.956 kb on - strand, within BT0310 at 373.003 kb on + strand, within BT0310 at 373.033 kb on + strand, within BT0310 at 373.033 kb on + strand, within BT0310 at 373.034 kb on - strand, within BT0310 at 373.034 kb on - strand, within BT0310 at 373.035 kb on + strand, within BT0310 at 373.172 kb on + strand, within BT0310 at 373.172 kb on + strand, within BT0310 at 373.177 kb on - strand, within BT0310 at 373.177 kb on - strand, within BT0310 at 373.177 kb on - strand, within BT0310 at 373.276 kb on + strand, within BT0310 at 373.332 kb on + strand, within BT0310 at 373.333 kb on - strand, within BT0310 at 373.378 kb on - strand, within BT0310 at 373.820 kb on - strand, within BT0311 at 373.830 kb on + strand, within BT0311
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 370,491 + -0.9 370,492 - +0.0 370,495 + +0.7 370,495 + -3.2 370,495 + -0.3 370,497 + -4.1 370,500 + -3.1 370,532 + -0.3 370,733 - +1.5 370,764 - +1.6 370,780 - +0.9 370,790 + BT0308 0.11 +0.1 370,931 + BT0308 0.31 +1.9 370,940 + BT0308 0.33 +0.5 370,996 - BT0308 0.41 +2.8 371,005 - BT0308 0.42 +2.1 371,006 + BT0308 0.42 +0.1 371,052 - BT0308 0.49 -0.1 371,161 + BT0308 0.64 +0.3 371,161 + BT0308 0.64 +1.0 371,162 - BT0308 0.64 -0.3 371,193 + BT0308 0.69 +2.1 371,193 + BT0308 0.69 -0.8 371,272 - BT0308 0.80 +2.5 371,298 - BT0308 0.83 +2.0 371,318 + BT0308 0.86 +2.7 371,432 - +0.4 371,489 - +0.5 371,506 + +3.0 371,507 - -3.6 371,507 - -2.3 371,595 + -1.9 371,623 + -2.3 371,623 + -0.3 371,643 + BT0309 0.11 -4.3 371,796 - BT0309 0.23 -1.1 371,954 - BT0309 0.35 -0.1 372,013 + BT0309 0.39 +2.0 372,015 + BT0309 0.39 -0.4 372,015 + BT0309 0.39 -0.3 372,143 - BT0309 0.49 +1.3 372,220 - BT0309 0.54 -1.2 372,242 - BT0309 0.56 +0.7 372,290 - BT0309 0.60 -3.5 372,322 - BT0309 0.62 -0.3 372,408 - BT0309 0.68 +1.6 372,488 + BT0309 0.74 -1.1 372,489 - BT0309 0.74 -0.3 372,541 + BT0309 0.78 -0.7 372,542 - BT0309 0.78 -0.1 372,586 + BT0309 0.82 +1.3 372,649 - BT0309 0.86 -0.9 372,661 - BT0309 0.87 +0.8 372,694 + BT0309 0.90 +1.0 372,695 - BT0309 0.90 -1.3 372,695 - BT0309 0.90 -0.3 372,739 - -3.1 372,797 - -0.1 372,932 - BT0310 0.16 +0.6 372,932 - BT0310 0.16 +0.7 372,956 - BT0310 0.19 -0.3 373,003 + BT0310 0.26 -0.5 373,033 + BT0310 0.30 +0.4 373,033 + BT0310 0.30 +0.9 373,034 - BT0310 0.30 +0.7 373,034 - BT0310 0.30 -0.1 373,035 + BT0310 0.30 -0.3 373,172 + BT0310 0.49 -0.3 373,172 + BT0310 0.49 +1.4 373,177 - BT0310 0.50 -1.3 373,177 - BT0310 0.50 +0.3 373,177 - BT0310 0.50 +1.4 373,276 + BT0310 0.64 -3.4 373,332 + BT0310 0.72 +1.1 373,333 - BT0310 0.72 +2.7 373,378 - BT0310 0.78 -2.1 373,820 - BT0311 0.14 +0.2 373,830 + BT0311 0.15 +1.2
Or see this region's nucleotide sequence