Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0254 and BT0255 are separated by 84 nucleotides BT0255 and BT0256 overlap by 1 nucleotides BT0256 and BT0257 are separated by 124 nucleotides
BT0254: BT0254 - putative Fe-S oxidoreductase (NCBI ptt file), at 282,585 to 284,447
BT0254
BT0255: BT0255 - hypothetical protein (NCBI ptt file), at 284,532 to 284,756
BT0255
BT0256: BT0256 - hypothetical protein (NCBI ptt file), at 284,756 to 285,073
BT0256
BT0257: BT0257 - xanthan lyase (NCBI ptt file), at 285,198 to 288,110
BT0257
Position (kb) 284
285
286 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 283.799 kb on - strand, within BT0254 at 283.894 kb on - strand, within BT0254 at 283.953 kb on + strand, within BT0254 at 283.953 kb on + strand, within BT0254 at 283.985 kb on - strand, within BT0254 at 284.028 kb on + strand, within BT0254 at 284.029 kb on - strand, within BT0254 at 284.032 kb on - strand, within BT0254 at 284.090 kb on - strand, within BT0254 at 284.184 kb on - strand, within BT0254 at 284.340 kb on + strand at 284.341 kb on - strand at 284.341 kb on - strand at 284.344 kb on + strand at 284.415 kb on - strand at 284.459 kb on - strand at 284.507 kb on + strand at 284.520 kb on + strand at 284.520 kb on + strand at 284.617 kb on + strand, within BT0255 at 284.625 kb on + strand, within BT0255 at 284.626 kb on - strand, within BT0255 at 284.723 kb on - strand, within BT0255 at 284.758 kb on - strand at 284.802 kb on + strand, within BT0256 at 284.843 kb on - strand, within BT0256 at 284.855 kb on - strand, within BT0256 at 284.855 kb on - strand, within BT0256 at 284.861 kb on + strand, within BT0256 at 284.862 kb on - strand, within BT0256 at 284.862 kb on - strand, within BT0256 at 284.862 kb on - strand, within BT0256 at 284.955 kb on - strand, within BT0256 at 284.995 kb on - strand, within BT0256 at 285.032 kb on + strand, within BT0256 at 285.033 kb on - strand, within BT0256 at 285.033 kb on - strand, within BT0256 at 285.062 kb on + strand at 285.064 kb on + strand at 285.065 kb on - strand at 285.065 kb on - strand at 285.065 kb on - strand at 285.067 kb on + strand at 285.068 kb on - strand at 285.087 kb on + strand at 285.087 kb on + strand at 285.097 kb on + strand at 285.097 kb on + strand at 285.097 kb on + strand at 285.182 kb on + strand at 285.351 kb on - strand at 285.351 kb on - strand at 285.407 kb on - strand at 285.407 kb on - strand at 285.565 kb on + strand, within BT0257 at 285.566 kb on - strand, within BT0257 at 285.689 kb on + strand, within BT0257 at 285.809 kb on - strand, within BT0257 at 285.828 kb on + strand, within BT0257 at 285.996 kb on - strand, within BT0257
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 283,799 - BT0254 0.65 +4.3 283,894 - BT0254 0.70 +1.9 283,953 + BT0254 0.73 -0.3 283,953 + BT0254 0.73 -0.1 283,985 - BT0254 0.75 -2.3 284,028 + BT0254 0.77 -0.1 284,029 - BT0254 0.78 +2.6 284,032 - BT0254 0.78 +1.1 284,090 - BT0254 0.81 +2.0 284,184 - BT0254 0.86 +0.1 284,340 + -0.9 284,341 - -0.5 284,341 - -4.1 284,344 + -0.4 284,415 - +1.9 284,459 - +0.1 284,507 + +0.0 284,520 + +0.1 284,520 + -2.0 284,617 + BT0255 0.38 -1.9 284,625 + BT0255 0.41 -0.7 284,626 - BT0255 0.42 -2.0 284,723 - BT0255 0.85 +0.9 284,758 - -0.3 284,802 + BT0256 0.14 -3.4 284,843 - BT0256 0.27 -1.9 284,855 - BT0256 0.31 -2.3 284,855 - BT0256 0.31 -2.3 284,861 + BT0256 0.33 -1.9 284,862 - BT0256 0.33 -1.6 284,862 - BT0256 0.33 -3.8 284,862 - BT0256 0.33 -0.3 284,955 - BT0256 0.63 -3.1 284,995 - BT0256 0.75 +0.9 285,032 + BT0256 0.87 -2.6 285,033 - BT0256 0.87 -0.7 285,033 - BT0256 0.87 -1.3 285,062 + -1.9 285,064 + -4.3 285,065 - +0.7 285,065 - -0.3 285,065 - -3.5 285,067 + +1.9 285,068 - -3.1 285,087 + -2.0 285,087 + -5.3 285,097 + -1.3 285,097 + -1.3 285,097 + -1.6 285,182 + -1.9 285,351 - -1.2 285,351 - -3.2 285,407 - -0.4 285,407 - +1.1 285,565 + BT0257 0.13 -1.9 285,566 - BT0257 0.13 +1.0 285,689 + BT0257 0.17 -3.5 285,809 - BT0257 0.21 -0.6 285,828 + BT0257 0.22 -1.6 285,996 - BT0257 0.27 -2.6
Or see this region's nucleotide sequence