Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0253 and BT0254 are separated by 441 nucleotides BT0254 and BT0255 are separated by 84 nucleotides BT0255 and BT0256 overlap by 1 nucleotides BT0256 and BT0257 are separated by 124 nucleotides
BT0253: BT0253 - hypothetical protein (NCBI ptt file), at 281,298 to 282,143
BT0253
BT0254: BT0254 - putative Fe-S oxidoreductase (NCBI ptt file), at 282,585 to 284,447
BT0254
BT0255: BT0255 - hypothetical protein (NCBI ptt file), at 284,532 to 284,756
BT0255
BT0256: BT0256 - hypothetical protein (NCBI ptt file), at 284,756 to 285,073
BT0256
BT0257: BT0257 - xanthan lyase (NCBI ptt file), at 285,198 to 288,110
BT0257
Position (kb)
282
283
284
285 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 281.629 kb on + strand, within BT0253 at 281.764 kb on - strand, within BT0253 at 281.778 kb on - strand, within BT0253 at 281.832 kb on - strand, within BT0253 at 281.885 kb on + strand, within BT0253 at 281.975 kb on + strand, within BT0253 at 281.992 kb on + strand, within BT0253 at 282.014 kb on + strand, within BT0253 at 282.036 kb on - strand, within BT0253 at 282.068 kb on - strand at 282.072 kb on - strand at 282.147 kb on - strand at 282.193 kb on - strand at 282.193 kb on - strand at 282.286 kb on + strand at 282.314 kb on + strand at 282.327 kb on + strand at 282.327 kb on + strand at 282.350 kb on - strand at 282.448 kb on - strand at 282.474 kb on - strand at 282.481 kb on + strand at 282.482 kb on - strand at 282.489 kb on + strand at 282.537 kb on - strand at 282.538 kb on + strand at 282.538 kb on + strand at 282.643 kb on - strand at 282.679 kb on + strand at 282.749 kb on + strand at 282.749 kb on + strand at 282.807 kb on - strand, within BT0254 at 282.903 kb on - strand, within BT0254 at 282.912 kb on + strand, within BT0254 at 282.915 kb on - strand, within BT0254 at 282.928 kb on - strand, within BT0254 at 283.084 kb on + strand at 283.084 kb on + strand, within BT0254 at 283.134 kb on + strand, within BT0254 at 283.135 kb on - strand, within BT0254 at 283.135 kb on - strand, within BT0254 at 283.200 kb on - strand, within BT0254 at 283.201 kb on + strand, within BT0254 at 283.202 kb on - strand, within BT0254 at 283.202 kb on - strand, within BT0254 at 283.203 kb on + strand, within BT0254 at 283.203 kb on + strand, within BT0254 at 283.232 kb on + strand, within BT0254 at 283.320 kb on - strand, within BT0254 at 283.348 kb on - strand, within BT0254 at 283.386 kb on + strand, within BT0254 at 283.514 kb on - strand, within BT0254 at 283.634 kb on + strand, within BT0254 at 283.641 kb on + strand, within BT0254 at 283.674 kb on - strand, within BT0254 at 283.799 kb on - strand, within BT0254 at 283.894 kb on - strand, within BT0254 at 283.953 kb on + strand, within BT0254 at 283.953 kb on + strand, within BT0254 at 283.985 kb on - strand, within BT0254 at 284.028 kb on + strand, within BT0254 at 284.029 kb on - strand, within BT0254 at 284.032 kb on - strand, within BT0254 at 284.090 kb on - strand, within BT0254 at 284.184 kb on - strand, within BT0254 at 284.340 kb on + strand at 284.341 kb on - strand at 284.341 kb on - strand at 284.344 kb on + strand at 284.415 kb on - strand at 284.459 kb on - strand at 284.507 kb on + strand at 284.520 kb on + strand at 284.520 kb on + strand at 284.617 kb on + strand, within BT0255 at 284.625 kb on + strand, within BT0255 at 284.626 kb on - strand, within BT0255 at 284.723 kb on - strand, within BT0255 at 284.758 kb on - strand at 284.802 kb on + strand, within BT0256 at 284.843 kb on - strand, within BT0256 at 284.855 kb on - strand, within BT0256 at 284.855 kb on - strand, within BT0256 at 284.861 kb on + strand, within BT0256 at 284.862 kb on - strand, within BT0256 at 284.862 kb on - strand, within BT0256 at 284.862 kb on - strand, within BT0256 at 284.955 kb on - strand, within BT0256 at 284.995 kb on - strand, within BT0256 at 285.032 kb on + strand, within BT0256 at 285.033 kb on - strand, within BT0256 at 285.033 kb on - strand, within BT0256 at 285.062 kb on + strand at 285.064 kb on + strand at 285.065 kb on - strand at 285.065 kb on - strand at 285.065 kb on - strand at 285.067 kb on + strand at 285.068 kb on - strand at 285.087 kb on + strand at 285.087 kb on + strand at 285.097 kb on + strand at 285.097 kb on + strand at 285.097 kb on + strand at 285.182 kb on + strand at 285.351 kb on - strand at 285.351 kb on - strand at 285.407 kb on - strand at 285.407 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 281,629 + BT0253 0.39 -1.3 281,764 - BT0253 0.55 -1.8 281,778 - BT0253 0.57 -1.2 281,832 - BT0253 0.63 +0.5 281,885 + BT0253 0.69 +0.5 281,975 + BT0253 0.80 -0.4 281,992 + BT0253 0.82 +3.0 282,014 + BT0253 0.85 -1.8 282,036 - BT0253 0.87 +1.3 282,068 - -0.5 282,072 - +0.3 282,147 - -0.8 282,193 - -1.3 282,193 - -0.8 282,286 + -1.2 282,314 + +3.4 282,327 + -0.3 282,327 + -1.3 282,350 - -0.3 282,448 - -0.3 282,474 - -0.8 282,481 + +0.7 282,482 - -0.3 282,489 + -1.0 282,537 - -2.5 282,538 + +2.2 282,538 + -1.0 282,643 - +2.5 282,679 + +0.7 282,749 + -0.3 282,749 + -2.6 282,807 - BT0254 0.12 +1.0 282,903 - BT0254 0.17 +2.4 282,912 + BT0254 0.18 +0.2 282,915 - BT0254 0.18 -0.1 282,928 - BT0254 0.18 +3.9 283,084 + -1.3 283,084 + BT0254 0.27 -0.3 283,134 + BT0254 0.29 -2.6 283,135 - BT0254 0.30 +3.3 283,135 - BT0254 0.30 -1.1 283,200 - BT0254 0.33 -1.5 283,201 + BT0254 0.33 +0.4 283,202 - BT0254 0.33 -0.1 283,202 - BT0254 0.33 +1.5 283,203 + BT0254 0.33 +1.3 283,203 + BT0254 0.33 -2.6 283,232 + BT0254 0.35 +3.3 283,320 - BT0254 0.39 -2.9 283,348 - BT0254 0.41 -1.3 283,386 + BT0254 0.43 +1.7 283,514 - BT0254 0.50 -0.9 283,634 + BT0254 0.56 +3.5 283,641 + BT0254 0.57 +0.5 283,674 - BT0254 0.58 +1.3 283,799 - BT0254 0.65 +4.3 283,894 - BT0254 0.70 +1.9 283,953 + BT0254 0.73 -0.3 283,953 + BT0254 0.73 -0.1 283,985 - BT0254 0.75 -2.3 284,028 + BT0254 0.77 -0.1 284,029 - BT0254 0.78 +2.6 284,032 - BT0254 0.78 +1.1 284,090 - BT0254 0.81 +2.0 284,184 - BT0254 0.86 +0.1 284,340 + -0.9 284,341 - -0.5 284,341 - -4.1 284,344 + -0.4 284,415 - +1.9 284,459 - +0.1 284,507 + +0.0 284,520 + +0.1 284,520 + -2.0 284,617 + BT0255 0.38 -1.9 284,625 + BT0255 0.41 -0.7 284,626 - BT0255 0.42 -2.0 284,723 - BT0255 0.85 +0.9 284,758 - -0.3 284,802 + BT0256 0.14 -3.4 284,843 - BT0256 0.27 -1.9 284,855 - BT0256 0.31 -2.3 284,855 - BT0256 0.31 -2.3 284,861 + BT0256 0.33 -1.9 284,862 - BT0256 0.33 -1.6 284,862 - BT0256 0.33 -3.8 284,862 - BT0256 0.33 -0.3 284,955 - BT0256 0.63 -3.1 284,995 - BT0256 0.75 +0.9 285,032 + BT0256 0.87 -2.6 285,033 - BT0256 0.87 -0.7 285,033 - BT0256 0.87 -1.3 285,062 + -1.9 285,064 + -4.3 285,065 - +0.7 285,065 - -0.3 285,065 - -3.5 285,067 + +1.9 285,068 - -3.1 285,087 + -2.0 285,087 + -5.3 285,097 + -1.3 285,097 + -1.3 285,097 + -1.6 285,182 + -1.9 285,351 - -1.2 285,351 - -3.2 285,407 - -0.4 285,407 - +1.1
Or see this region's nucleotide sequence