Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT0122

Experiment: BHIS with Chlorpromazine 0.1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT0121 and tRNA-Ser are separated by 523 nucleotidestRNA-Ser and BT0122 are separated by 174 nucleotidesBT0122 and BT0123 are separated by 50 nucleotidesBT0123 and BT0124 are separated by 18 nucleotides BT0121: BT0121 - transcriptional regulator, ArsR family (NCBI ptt file), at 110,593 to 110,862 BT0121 BT_t01: tRNA-Ser - tRNA-Ser (RefSeq), at 111,386 to 111,473 tRNA-Ser BT0122: BT0122 - 3-oxoacyl-[acyl-carrier-protein] synthase III (NCBI ptt file), at 111,648 to 112,655 BT0122 BT0123: BT0123 - NADH-ubiquinone oxidoreductase subunit (NCBI ptt file), at 112,706 to 113,182 BT0123 BT0124: BT0124 - NADH:ubiquinone oxidoreductase subunit (NCBI ptt file), at 113,201 to 114,967 BT0124 Position (kb) 111 112 113Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 110.780 kb on - strand, within BT0121at 110.825 kb on - strand, within BT0121at 110.825 kb on - strand, within BT0121at 110.862 kb on + strandat 110.868 kb on + strandat 110.904 kb on - strandat 110.923 kb on + strandat 110.923 kb on + strandat 110.924 kb on - strandat 111.001 kb on + strandat 111.036 kb on + strandat 111.038 kb on + strandat 111.039 kb on - strandat 111.039 kb on - strandat 111.043 kb on - strandat 111.043 kb on - strandat 111.045 kb on - strandat 111.047 kb on - strandat 111.069 kb on - strandat 111.311 kb on + strandat 111.554 kb on + strandat 111.569 kb on + strandat 111.699 kb on + strandat 111.772 kb on + strand, within BT0122at 111.816 kb on + strand, within BT0122at 111.817 kb on - strand, within BT0122at 111.817 kb on - strand, within BT0122at 112.050 kb on + strand, within BT0122at 112.062 kb on - strand, within BT0122at 112.295 kb on + strand, within BT0122at 112.295 kb on + strand, within BT0122at 112.361 kb on + strand, within BT0122at 112.386 kb on - strand, within BT0122at 112.658 kb on + strandat 112.659 kb on - strandat 112.981 kb on - strand, within BT0123at 112.981 kb on - strand, within BT0123at 113.185 kb on + strandat 113.186 kb on - strandat 113.186 kb on - strandat 113.186 kb on - strandat 113.188 kb on + strandat 113.189 kb on - strandat 113.189 kb on - strandat 113.189 kb on - strandat 113.224 kb on - strandat 113.284 kb on - strandat 113.298 kb on + strandat 113.461 kb on + strand, within BT0124at 113.462 kb on - strand, within BT0124at 113.477 kb on - strand, within BT0124at 113.483 kb on + strand, within BT0124at 113.504 kb on + strand, within BT0124at 113.582 kb on + strand, within BT0124at 113.623 kb on - strand, within BT0124

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM
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110,780 - BT0121 0.69 +1.3
110,825 - BT0121 0.86 -0.2
110,825 - BT0121 0.86 -2.9
110,862 + -1.6
110,868 + -3.0
110,904 - +2.6
110,923 + -0.5
110,923 + -4.1
110,924 - -2.5
111,001 + +1.6
111,036 + -0.9
111,038 + +1.8
111,039 - -2.5
111,039 - -0.3
111,043 - -2.5
111,043 - -1.6
111,045 - -1.6
111,047 - +2.0
111,069 - -4.6
111,311 + -0.2
111,554 + -4.7
111,569 + -1.6
111,699 + -3.7
111,772 + BT0122 0.12 -2.5
111,816 + BT0122 0.17 -0.2
111,817 - BT0122 0.17 -3.8
111,817 - BT0122 0.17 -4.6
112,050 + BT0122 0.40 -2.2
112,062 - BT0122 0.41 -2.0
112,295 + BT0122 0.64 -2.8
112,295 + BT0122 0.64 -4.0
112,361 + BT0122 0.71 -3.2
112,386 - BT0122 0.73 -1.8
112,658 + -0.6
112,659 - -1.5
112,981 - BT0123 0.58 -0.2
112,981 - BT0123 0.58 -0.2
113,185 + +0.4
113,186 - -0.9
113,186 - -0.8
113,186 - -1.1
113,188 + -2.7
113,189 - +0.8
113,189 - +2.1
113,189 - -0.2
113,224 - -2.2
113,284 - -1.2
113,298 + -1.2
113,461 + BT0124 0.15 -0.2
113,462 - BT0124 0.15 -0.6
113,477 - BT0124 0.16 -0.7
113,483 + BT0124 0.16 +0.5
113,504 + BT0124 0.17 +0.3
113,582 + BT0124 0.22 +0.9
113,623 - BT0124 0.24 +2.6

Or see this region's nucleotide sequence