Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0121 and tRNA-Ser are separated by 523 nucleotides tRNA-Ser and BT0122 are separated by 174 nucleotides BT0122 and BT0123 are separated by 50 nucleotides BT0123 and BT0124 are separated by 18 nucleotides
BT0121: BT0121 - transcriptional regulator, ArsR family (NCBI ptt file), at 110,593 to 110,862
BT0121
BT_t01: tRNA-Ser - tRNA-Ser (RefSeq), at 111,386 to 111,473
tRNA-Ser
BT0122: BT0122 - 3-oxoacyl-[acyl-carrier-protein] synthase III (NCBI ptt file), at 111,648 to 112,655
BT0122
BT0123: BT0123 - NADH-ubiquinone oxidoreductase subunit (NCBI ptt file), at 112,706 to 113,182
BT0123
BT0124: BT0124 - NADH:ubiquinone oxidoreductase subunit (NCBI ptt file), at 113,201 to 114,967
BT0124
Position (kb)
111
112
113 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 110.780 kb on - strand, within BT0121 at 110.825 kb on - strand, within BT0121 at 110.825 kb on - strand, within BT0121 at 110.862 kb on + strand at 110.868 kb on + strand at 110.904 kb on - strand at 110.923 kb on + strand at 110.923 kb on + strand at 110.924 kb on - strand at 111.001 kb on + strand at 111.036 kb on + strand at 111.038 kb on + strand at 111.039 kb on - strand at 111.039 kb on - strand at 111.043 kb on - strand at 111.043 kb on - strand at 111.045 kb on - strand at 111.047 kb on - strand at 111.069 kb on - strand at 111.311 kb on + strand at 111.554 kb on + strand at 111.569 kb on + strand at 111.699 kb on + strand at 111.772 kb on + strand, within BT0122 at 111.816 kb on + strand, within BT0122 at 111.817 kb on - strand, within BT0122 at 111.817 kb on - strand, within BT0122 at 112.050 kb on + strand, within BT0122 at 112.062 kb on - strand, within BT0122 at 112.295 kb on + strand, within BT0122 at 112.295 kb on + strand, within BT0122 at 112.361 kb on + strand, within BT0122 at 112.386 kb on - strand, within BT0122 at 112.658 kb on + strand at 112.659 kb on - strand at 112.981 kb on - strand, within BT0123 at 112.981 kb on - strand, within BT0123 at 113.185 kb on + strand at 113.186 kb on - strand at 113.186 kb on - strand at 113.186 kb on - strand at 113.188 kb on + strand at 113.189 kb on - strand at 113.189 kb on - strand at 113.189 kb on - strand at 113.224 kb on - strand at 113.284 kb on - strand at 113.298 kb on + strand at 113.461 kb on + strand, within BT0124 at 113.462 kb on - strand, within BT0124 at 113.477 kb on - strand, within BT0124 at 113.483 kb on + strand, within BT0124 at 113.504 kb on + strand, within BT0124 at 113.582 kb on + strand, within BT0124 at 113.623 kb on - strand, within BT0124
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 110,780 - BT0121 0.69 +1.3 110,825 - BT0121 0.86 -0.2 110,825 - BT0121 0.86 -2.9 110,862 + -1.6 110,868 + -3.0 110,904 - +2.6 110,923 + -0.5 110,923 + -4.1 110,924 - -2.5 111,001 + +1.6 111,036 + -0.9 111,038 + +1.8 111,039 - -2.5 111,039 - -0.3 111,043 - -2.5 111,043 - -1.6 111,045 - -1.6 111,047 - +2.0 111,069 - -4.6 111,311 + -0.2 111,554 + -4.7 111,569 + -1.6 111,699 + -3.7 111,772 + BT0122 0.12 -2.5 111,816 + BT0122 0.17 -0.2 111,817 - BT0122 0.17 -3.8 111,817 - BT0122 0.17 -4.6 112,050 + BT0122 0.40 -2.2 112,062 - BT0122 0.41 -2.0 112,295 + BT0122 0.64 -2.8 112,295 + BT0122 0.64 -4.0 112,361 + BT0122 0.71 -3.2 112,386 - BT0122 0.73 -1.8 112,658 + -0.6 112,659 - -1.5 112,981 - BT0123 0.58 -0.2 112,981 - BT0123 0.58 -0.2 113,185 + +0.4 113,186 - -0.9 113,186 - -0.8 113,186 - -1.1 113,188 + -2.7 113,189 - +0.8 113,189 - +2.1 113,189 - -0.2 113,224 - -2.2 113,284 - -1.2 113,298 + -1.2 113,461 + BT0124 0.15 -0.2 113,462 - BT0124 0.15 -0.6 113,477 - BT0124 0.16 -0.7 113,483 + BT0124 0.16 +0.5 113,504 + BT0124 0.17 +0.3 113,582 + BT0124 0.22 +0.9 113,623 - BT0124 0.24 +2.6
Or see this region's nucleotide sequence