Experiment: MRS + kanamycin Time8hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12715 and BBR_RS12720 are separated by 181 nucleotides BBR_RS12720 and BBR_RS12725 are separated by 140 nucleotides BBR_RS12725 and BBR_RS20515 are separated by 64 nucleotides
BBR_RS12715: BBR_RS12715 - helix-turn-helix domain-containing protein, at 607,399 to 607,782
_RS12715
BBR_RS12720: BBR_RS12720 - hypothetical protein, at 607,964 to 608,050
_RS12720
BBR_RS12725: BBR_RS12725 - hypothetical protein, at 608,191 to 608,571
_RS12725
BBR_RS20515: BBR_RS20515 - transposase, at 608,636 to 609,697
_RS20515
Position (kb) 608
609 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 607.211 kb on - strand at 607.216 kb on + strand at 607.224 kb on - strand at 607.229 kb on - strand at 607.256 kb on + strand at 607.256 kb on + strand at 607.305 kb on - strand at 607.310 kb on + strand at 607.401 kb on + strand at 607.401 kb on + strand at 607.409 kb on - strand at 607.432 kb on - strand at 607.437 kb on - strand at 607.437 kb on - strand at 607.442 kb on - strand, within BBR_RS12715 at 607.456 kb on - strand, within BBR_RS12715 at 607.468 kb on - strand, within BBR_RS12715 at 607.518 kb on + strand, within BBR_RS12715 at 607.540 kb on + strand, within BBR_RS12715 at 607.543 kb on + strand, within BBR_RS12715 at 607.577 kb on + strand, within BBR_RS12715 at 607.577 kb on + strand, within BBR_RS12715 at 607.597 kb on + strand, within BBR_RS12715 at 607.600 kb on - strand, within BBR_RS12715 at 607.600 kb on - strand, within BBR_RS12715 at 607.640 kb on + strand, within BBR_RS12715 at 607.653 kb on - strand, within BBR_RS12715 at 607.668 kb on + strand, within BBR_RS12715 at 607.676 kb on - strand, within BBR_RS12715 at 607.759 kb on - strand at 607.791 kb on + strand at 607.800 kb on - strand at 607.818 kb on + strand at 607.927 kb on - strand at 608.020 kb on - strand, within BBR_RS12720 at 608.021 kb on - strand, within BBR_RS12720 at 608.031 kb on - strand, within BBR_RS12720 at 608.117 kb on - strand at 608.117 kb on - strand at 608.123 kb on - strand at 608.123 kb on - strand at 608.123 kb on - strand at 608.123 kb on - strand at 608.152 kb on + strand at 608.181 kb on - strand at 608.191 kb on - strand at 608.191 kb on - strand at 608.191 kb on - strand at 608.191 kb on - strand at 608.193 kb on + strand at 608.201 kb on - strand at 608.201 kb on - strand at 608.211 kb on + strand at 608.231 kb on + strand, within BBR_RS12725 at 608.239 kb on - strand, within BBR_RS12725 at 608.347 kb on + strand, within BBR_RS12725 at 608.355 kb on - strand, within BBR_RS12725 at 608.357 kb on - strand, within BBR_RS12725 at 608.357 kb on - strand, within BBR_RS12725 at 608.379 kb on + strand, within BBR_RS12725 at 608.382 kb on - strand, within BBR_RS12725 at 608.397 kb on + strand, within BBR_RS12725 at 608.405 kb on - strand, within BBR_RS12725 at 608.424 kb on - strand, within BBR_RS12725 at 608.502 kb on - strand, within BBR_RS12725 at 608.553 kb on + strand at 608.555 kb on + strand at 608.555 kb on + strand at 608.555 kb on + strand at 608.561 kb on - strand at 608.563 kb on - strand at 608.563 kb on - strand at 608.599 kb on + strand at 608.602 kb on + strand at 608.604 kb on + strand at 608.662 kb on - strand at 608.663 kb on + strand at 608.663 kb on + strand at 608.671 kb on - strand at 608.676 kb on - strand at 608.676 kb on - strand at 608.676 kb on - strand at 608.676 kb on - strand at 608.676 kb on - strand at 608.676 kb on - strand at 608.749 kb on - strand, within BBR_RS20515 at 608.767 kb on + strand, within BBR_RS20515 at 608.767 kb on - strand, within BBR_RS20515 at 608.804 kb on - strand, within BBR_RS20515 at 608.841 kb on + strand, within BBR_RS20515 at 608.841 kb on + strand, within BBR_RS20515 at 608.864 kb on - strand, within BBR_RS20515 at 608.864 kb on - strand, within BBR_RS20515 at 608.866 kb on + strand, within BBR_RS20515 at 608.935 kb on + strand, within BBR_RS20515 at 608.942 kb on + strand, within BBR_RS20515 at 608.942 kb on + strand, within BBR_RS20515 at 608.950 kb on - strand, within BBR_RS20515 at 608.950 kb on - strand, within BBR_RS20515 at 608.960 kb on - strand, within BBR_RS20515 at 608.973 kb on + strand, within BBR_RS20515 at 608.973 kb on + strand, within BBR_RS20515 at 608.978 kb on + strand, within BBR_RS20515 at 608.981 kb on - strand, within BBR_RS20515 at 608.981 kb on - strand, within BBR_RS20515 at 608.981 kb on - strand, within BBR_RS20515 at 609.043 kb on - strand, within BBR_RS20515 at 609.099 kb on + strand, within BBR_RS20515 at 609.111 kb on + strand, within BBR_RS20515 at 609.163 kb on - strand, within BBR_RS20515 at 609.206 kb on - strand, within BBR_RS20515 at 609.221 kb on - strand, within BBR_RS20515 at 609.238 kb on + strand, within BBR_RS20515 at 609.246 kb on - strand, within BBR_RS20515 at 609.281 kb on + strand, within BBR_RS20515 at 609.281 kb on + strand, within BBR_RS20515 at 609.293 kb on + strand, within BBR_RS20515 at 609.301 kb on - strand, within BBR_RS20515 at 609.301 kb on - strand, within BBR_RS20515 at 609.309 kb on + strand, within BBR_RS20515 at 609.316 kb on - strand, within BBR_RS20515 at 609.317 kb on - strand, within BBR_RS20515 at 609.330 kb on + strand, within BBR_RS20515 at 609.351 kb on + strand, within BBR_RS20515 at 609.359 kb on - strand, within BBR_RS20515 at 609.359 kb on - strand, within BBR_RS20515 at 609.371 kb on - strand, within BBR_RS20515 at 609.457 kb on + strand, within BBR_RS20515 at 609.481 kb on + strand, within BBR_RS20515 at 609.546 kb on - strand, within BBR_RS20515
Per-strain Table
Position Strand Gene LocusTag Fraction MRS + kanamycin Time8hr remove 607,211 - +1.1 607,216 + -0.0 607,224 - -2.8 607,229 - +0.2 607,256 + +0.6 607,256 + +1.0 607,305 - +3.0 607,310 + +0.8 607,401 + +0.3 607,401 + -1.2 607,409 - +0.7 607,432 - -1.4 607,437 - -0.8 607,437 - +2.1 607,442 - BBR_RS12715 0.11 -0.3 607,456 - BBR_RS12715 0.15 -0.7 607,468 - BBR_RS12715 0.18 -1.4 607,518 + BBR_RS12715 0.31 +0.8 607,540 + BBR_RS12715 0.37 +0.7 607,543 + BBR_RS12715 0.38 +0.7 607,577 + BBR_RS12715 0.46 -0.1 607,577 + BBR_RS12715 0.46 -2.2 607,597 + BBR_RS12715 0.52 +0.0 607,600 - BBR_RS12715 0.52 +2.2 607,600 - BBR_RS12715 0.52 +1.6 607,640 + BBR_RS12715 0.63 -2.2 607,653 - BBR_RS12715 0.66 +1.9 607,668 + BBR_RS12715 0.70 -3.7 607,676 - BBR_RS12715 0.72 -1.2 607,759 - -2.2 607,791 + -0.4 607,800 - -1.2 607,818 + +2.6 607,927 - +2.3 608,020 - BBR_RS12720 0.64 -0.5 608,021 - BBR_RS12720 0.66 -1.4 608,031 - BBR_RS12720 0.77 +1.7 608,117 - -1.4 608,117 - +0.8 608,123 - +0.3 608,123 - -1.2 608,123 - +0.9 608,123 - +0.3 608,152 + -1.2 608,181 - +2.2 608,191 - -1.2 608,191 - +1.6 608,191 - -0.8 608,191 - -0.2 608,193 + +2.3 608,201 - -0.7 608,201 - -0.3 608,211 + +0.7 608,231 + BBR_RS12725 0.10 +1.1 608,239 - BBR_RS12725 0.13 +1.3 608,347 + BBR_RS12725 0.41 +0.3 608,355 - BBR_RS12725 0.43 +0.3 608,357 - BBR_RS12725 0.44 +1.1 608,357 - BBR_RS12725 0.44 -1.7 608,379 + BBR_RS12725 0.49 +2.1 608,382 - BBR_RS12725 0.50 +0.7 608,397 + BBR_RS12725 0.54 +0.3 608,405 - BBR_RS12725 0.56 +0.4 608,424 - BBR_RS12725 0.61 -0.3 608,502 - BBR_RS12725 0.82 +1.7 608,553 + +0.1 608,555 + -1.0 608,555 + -0.5 608,555 + -0.6 608,561 - -0.3 608,563 - +0.7 608,563 - -0.7 608,599 + +1.9 608,602 + +2.2 608,604 + +2.3 608,662 - -1.7 608,663 + +0.1 608,663 + -3.6 608,671 - +0.3 608,676 - -0.4 608,676 - -1.8 608,676 - -0.7 608,676 - -1.7 608,676 - -1.5 608,676 - -0.3 608,749 - BBR_RS20515 0.11 -0.1 608,767 + BBR_RS20515 0.12 +0.6 608,767 - BBR_RS20515 0.12 -0.3 608,804 - BBR_RS20515 0.16 +1.7 608,841 + BBR_RS20515 0.19 -2.3 608,841 + BBR_RS20515 0.19 -1.3 608,864 - BBR_RS20515 0.21 -1.1 608,864 - BBR_RS20515 0.21 -0.1 608,866 + BBR_RS20515 0.22 -0.0 608,935 + BBR_RS20515 0.28 -2.4 608,942 + BBR_RS20515 0.29 +1.9 608,942 + BBR_RS20515 0.29 -0.3 608,950 - BBR_RS20515 0.30 +0.6 608,950 - BBR_RS20515 0.30 -0.3 608,960 - BBR_RS20515 0.31 -1.6 608,973 + BBR_RS20515 0.32 -1.5 608,973 + BBR_RS20515 0.32 +0.4 608,978 + BBR_RS20515 0.32 -0.4 608,981 - BBR_RS20515 0.32 +1.7 608,981 - BBR_RS20515 0.32 +0.3 608,981 - BBR_RS20515 0.32 -0.0 609,043 - BBR_RS20515 0.38 +0.5 609,099 + BBR_RS20515 0.44 -2.3 609,111 + BBR_RS20515 0.45 -0.8 609,163 - BBR_RS20515 0.50 -2.5 609,206 - BBR_RS20515 0.54 -0.3 609,221 - BBR_RS20515 0.55 -0.0 609,238 + BBR_RS20515 0.57 +0.9 609,246 - BBR_RS20515 0.57 +0.2 609,281 + BBR_RS20515 0.61 +3.4 609,281 + BBR_RS20515 0.61 -1.5 609,293 + BBR_RS20515 0.62 +0.3 609,301 - BBR_RS20515 0.63 -1.3 609,301 - BBR_RS20515 0.63 -0.3 609,309 + BBR_RS20515 0.63 -0.8 609,316 - BBR_RS20515 0.64 +0.3 609,317 - BBR_RS20515 0.64 -0.4 609,330 + BBR_RS20515 0.65 +1.4 609,351 + BBR_RS20515 0.67 -0.8 609,359 - BBR_RS20515 0.68 +2.4 609,359 - BBR_RS20515 0.68 -0.3 609,371 - BBR_RS20515 0.69 +2.2 609,457 + BBR_RS20515 0.77 +3.3 609,481 + BBR_RS20515 0.80 -0.6 609,546 - BBR_RS20515 0.86 +0.7
Or see this region's nucleotide sequence