Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_36 and Pf6N2E2_37 are separated by 233 nucleotides Pf6N2E2_37 and Pf6N2E2_38 are separated by 83 nucleotides Pf6N2E2_38 and Pf6N2E2_39 are separated by 143 nucleotides Pf6N2E2_39 and Pf6N2E2_40 overlap by 1 nucleotides Pf6N2E2_40 and Pf6N2E2_41 overlap by 1 nucleotides
Pf6N2E2_36 - Possible oxidoreductase, at 75,774 to 76,604
_36
Pf6N2E2_37 - 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I, at 76,838 to 77,194
_37
Pf6N2E2_38 - FIG00954456: hypothetical protein, at 77,278 to 78,357
_38
Pf6N2E2_39 - FIG00857858: hypothetical protein, at 78,501 to 78,809
_39
Pf6N2E2_40 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 78,809 to 79,120
_40
Pf6N2E2_41 - Two-component system response regulator QseB, at 79,120 to 79,788
_41
Position (kb)
77
78
79 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 76.438 kb on + strand, within Pf6N2E2_36 at 76.438 kb on + strand, within Pf6N2E2_36 at 76.438 kb on + strand, within Pf6N2E2_36 at 76.438 kb on + strand, within Pf6N2E2_36 at 76.438 kb on + strand, within Pf6N2E2_36 at 76.438 kb on + strand, within Pf6N2E2_36 at 76.438 kb on + strand, within Pf6N2E2_36 at 76.438 kb on + strand, within Pf6N2E2_36 at 76.439 kb on - strand, within Pf6N2E2_36 at 76.439 kb on - strand, within Pf6N2E2_36 at 76.439 kb on - strand, within Pf6N2E2_36 at 76.439 kb on - strand, within Pf6N2E2_36 at 76.439 kb on - strand, within Pf6N2E2_36 at 76.439 kb on - strand, within Pf6N2E2_36 at 76.439 kb on - strand, within Pf6N2E2_36 at 76.439 kb on - strand, within Pf6N2E2_36 at 76.489 kb on + strand, within Pf6N2E2_36 at 76.489 kb on + strand, within Pf6N2E2_36 at 76.489 kb on + strand, within Pf6N2E2_36 at 76.490 kb on - strand, within Pf6N2E2_36 at 76.490 kb on - strand, within Pf6N2E2_36 at 76.576 kb on + strand at 76.577 kb on - strand at 76.577 kb on - strand at 76.644 kb on + strand at 76.645 kb on - strand at 76.645 kb on - strand at 76.739 kb on + strand at 76.739 kb on + strand at 76.740 kb on - strand at 76.851 kb on + strand at 76.851 kb on + strand at 76.852 kb on - strand at 76.852 kb on - strand at 76.852 kb on - strand at 76.852 kb on - strand at 76.852 kb on - strand at 76.986 kb on + strand, within Pf6N2E2_37 at 76.986 kb on + strand, within Pf6N2E2_37 at 76.986 kb on + strand, within Pf6N2E2_37 at 76.986 kb on + strand, within Pf6N2E2_37 at 76.987 kb on - strand, within Pf6N2E2_37 at 76.987 kb on - strand, within Pf6N2E2_37 at 76.987 kb on - strand, within Pf6N2E2_37 at 77.007 kb on + strand, within Pf6N2E2_37 at 77.209 kb on + strand at 77.209 kb on + strand at 77.210 kb on - strand at 77.242 kb on + strand at 77.243 kb on - strand at 77.280 kb on - strand at 77.376 kb on - strand at 77.422 kb on - strand, within Pf6N2E2_38 at 77.459 kb on + strand, within Pf6N2E2_38 at 77.460 kb on - strand, within Pf6N2E2_38 at 77.597 kb on + strand, within Pf6N2E2_38 at 77.598 kb on - strand, within Pf6N2E2_38 at 77.616 kb on - strand, within Pf6N2E2_38 at 77.639 kb on + strand, within Pf6N2E2_38 at 78.065 kb on + strand, within Pf6N2E2_38 at 78.065 kb on + strand, within Pf6N2E2_38 at 78.065 kb on + strand, within Pf6N2E2_38 at 78.065 kb on + strand, within Pf6N2E2_38 at 78.065 kb on + strand, within Pf6N2E2_38 at 78.065 kb on + strand, within Pf6N2E2_38 at 78.065 kb on + strand, within Pf6N2E2_38 at 78.066 kb on - strand, within Pf6N2E2_38 at 78.066 kb on - strand, within Pf6N2E2_38 at 78.066 kb on - strand, within Pf6N2E2_38 at 78.388 kb on + strand at 78.389 kb on - strand at 78.389 kb on - strand at 78.389 kb on - strand at 78.390 kb on + strand at 78.391 kb on - strand at 78.409 kb on + strand at 78.410 kb on - strand at 78.410 kb on - strand at 78.410 kb on - strand at 78.410 kb on - strand at 78.482 kb on + strand at 78.483 kb on - strand at 78.483 kb on - strand at 78.484 kb on + strand at 78.484 kb on + strand at 78.484 kb on + strand at 78.485 kb on - strand at 78.485 kb on - strand at 78.485 kb on - strand at 78.485 kb on - strand at 78.485 kb on - strand at 78.485 kb on - strand at 78.485 kb on - strand at 78.495 kb on + strand at 78.611 kb on + strand, within Pf6N2E2_39 at 78.708 kb on + strand, within Pf6N2E2_39 at 78.708 kb on + strand, within Pf6N2E2_39 at 78.709 kb on - strand, within Pf6N2E2_39 at 78.709 kb on - strand, within Pf6N2E2_39 at 78.714 kb on + strand, within Pf6N2E2_39 at 78.714 kb on + strand, within Pf6N2E2_39 at 78.715 kb on - strand, within Pf6N2E2_39 at 78.715 kb on - strand, within Pf6N2E2_39 at 78.735 kb on - strand, within Pf6N2E2_39 at 78.804 kb on - strand at 78.807 kb on + strand at 78.808 kb on - strand at 78.808 kb on - strand at 78.833 kb on - strand at 79.023 kb on - strand, within Pf6N2E2_40 at 79.023 kb on - strand, within Pf6N2E2_40 at 79.023 kb on - strand, within Pf6N2E2_40 at 79.025 kb on - strand, within Pf6N2E2_40 at 79.025 kb on - strand, within Pf6N2E2_40 at 79.025 kb on - strand, within Pf6N2E2_40 at 79.029 kb on - strand, within Pf6N2E2_40 at 79.099 kb on - strand at 79.108 kb on + strand at 79.109 kb on - strand at 79.230 kb on - strand, within Pf6N2E2_41 at 79.232 kb on - strand, within Pf6N2E2_41 at 79.252 kb on + strand, within Pf6N2E2_41 at 79.253 kb on - strand, within Pf6N2E2_41
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 76,438 + Pf6N2E2_36 0.80 +1.4 76,438 + Pf6N2E2_36 0.80 -0.4 76,438 + Pf6N2E2_36 0.80 -1.0 76,438 + Pf6N2E2_36 0.80 +0.3 76,438 + Pf6N2E2_36 0.80 -1.8 76,438 + Pf6N2E2_36 0.80 -0.5 76,438 + Pf6N2E2_36 0.80 -1.0 76,438 + Pf6N2E2_36 0.80 -2.8 76,439 - Pf6N2E2_36 0.80 -0.0 76,439 - Pf6N2E2_36 0.80 +0.5 76,439 - Pf6N2E2_36 0.80 +0.2 76,439 - Pf6N2E2_36 0.80 +0.3 76,439 - Pf6N2E2_36 0.80 +1.7 76,439 - Pf6N2E2_36 0.80 -1.6 76,439 - Pf6N2E2_36 0.80 +2.1 76,439 - Pf6N2E2_36 0.80 +1.0 76,489 + Pf6N2E2_36 0.86 -0.4 76,489 + Pf6N2E2_36 0.86 +0.5 76,489 + Pf6N2E2_36 0.86 +0.3 76,490 - Pf6N2E2_36 0.86 +0.7 76,490 - Pf6N2E2_36 0.86 +1.7 76,576 + +1.0 76,577 - -0.5 76,577 - -0.3 76,644 + -2.3 76,645 - +0.8 76,645 - -1.5 76,739 + +0.7 76,739 + +0.1 76,740 - -0.3 76,851 + +1.2 76,851 + -0.4 76,852 - +1.2 76,852 - +1.2 76,852 - +0.7 76,852 - +1.6 76,852 - +1.6 76,986 + Pf6N2E2_37 0.41 -3.3 76,986 + Pf6N2E2_37 0.41 -2.1 76,986 + Pf6N2E2_37 0.41 -1.6 76,986 + Pf6N2E2_37 0.41 -0.8 76,987 - Pf6N2E2_37 0.42 -0.8 76,987 - Pf6N2E2_37 0.42 -1.8 76,987 - Pf6N2E2_37 0.42 -2.0 77,007 + Pf6N2E2_37 0.47 -1.3 77,209 + -0.8 77,209 + +1.5 77,210 - -0.5 77,242 + +1.7 77,243 - +3.1 77,280 - +0.2 77,376 - +0.7 77,422 - Pf6N2E2_38 0.13 +1.0 77,459 + Pf6N2E2_38 0.17 +1.4 77,460 - Pf6N2E2_38 0.17 -0.1 77,597 + Pf6N2E2_38 0.30 -1.0 77,598 - Pf6N2E2_38 0.30 -0.8 77,616 - Pf6N2E2_38 0.31 -0.3 77,639 + Pf6N2E2_38 0.33 +0.7 78,065 + Pf6N2E2_38 0.73 -0.1 78,065 + Pf6N2E2_38 0.73 -2.7 78,065 + Pf6N2E2_38 0.73 -0.4 78,065 + Pf6N2E2_38 0.73 +1.3 78,065 + Pf6N2E2_38 0.73 +1.1 78,065 + Pf6N2E2_38 0.73 -0.4 78,065 + Pf6N2E2_38 0.73 +1.4 78,066 - Pf6N2E2_38 0.73 -0.2 78,066 - Pf6N2E2_38 0.73 +0.8 78,066 - Pf6N2E2_38 0.73 +0.4 78,388 + +1.1 78,389 - -0.3 78,389 - -1.5 78,389 - +0.2 78,390 + -1.8 78,391 - -0.4 78,409 + -0.8 78,410 - -0.1 78,410 - +0.7 78,410 - +0.5 78,410 - -1.8 78,482 + +0.2 78,483 - +0.8 78,483 - +1.6 78,484 + -0.1 78,484 + -0.2 78,484 + +0.7 78,485 - +0.1 78,485 - +0.5 78,485 - +1.2 78,485 - -0.1 78,485 - +1.1 78,485 - +0.9 78,485 - -2.2 78,495 + +1.0 78,611 + Pf6N2E2_39 0.36 -0.4 78,708 + Pf6N2E2_39 0.67 +0.8 78,708 + Pf6N2E2_39 0.67 +0.8 78,709 - Pf6N2E2_39 0.67 -2.1 78,709 - Pf6N2E2_39 0.67 -0.6 78,714 + Pf6N2E2_39 0.69 +1.6 78,714 + Pf6N2E2_39 0.69 -0.5 78,715 - Pf6N2E2_39 0.69 +1.2 78,715 - Pf6N2E2_39 0.69 +0.5 78,735 - Pf6N2E2_39 0.76 +2.4 78,804 - +1.6 78,807 + -0.9 78,808 - +0.2 78,808 - +1.0 78,833 - -1.8 79,023 - Pf6N2E2_40 0.69 -2.2 79,023 - Pf6N2E2_40 0.69 -1.3 79,023 - Pf6N2E2_40 0.69 +0.2 79,025 - Pf6N2E2_40 0.69 -0.1 79,025 - Pf6N2E2_40 0.69 -0.6 79,025 - Pf6N2E2_40 0.69 +0.7 79,029 - Pf6N2E2_40 0.71 -0.5 79,099 - -2.2 79,108 + +0.2 79,109 - -3.0 79,230 - Pf6N2E2_41 0.16 -1.2 79,232 - Pf6N2E2_41 0.17 +0.6 79,252 + Pf6N2E2_41 0.20 +0.5 79,253 - Pf6N2E2_41 0.20 -1.8
Or see this region's nucleotide sequence