Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_36

Experiment: L-Leucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_35 and Pf6N2E2_36 are separated by 186 nucleotidesPf6N2E2_36 and Pf6N2E2_37 are separated by 233 nucleotidesPf6N2E2_37 and Pf6N2E2_38 are separated by 83 nucleotides Pf6N2E2_35 - Cytosine permease, at 74,316 to 75,587 _35 Pf6N2E2_36 - Possible oxidoreductase, at 75,774 to 76,604 _36 Pf6N2E2_37 - 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I, at 76,838 to 77,194 _37 Pf6N2E2_38 - FIG00954456: hypothetical protein, at 77,278 to 78,357 _38 Position (kb) 75 76 77Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 74.821 kb on + strand, within Pf6N2E2_35at 74.821 kb on + strand, within Pf6N2E2_35at 74.821 kb on + strand, within Pf6N2E2_35at 74.821 kb on + strand, within Pf6N2E2_35at 74.821 kb on + strand, within Pf6N2E2_35at 74.822 kb on - strand, within Pf6N2E2_35at 75.107 kb on - strand, within Pf6N2E2_35at 75.107 kb on - strand, within Pf6N2E2_35at 75.139 kb on + strand, within Pf6N2E2_35at 75.140 kb on - strand, within Pf6N2E2_35at 75.140 kb on - strand, within Pf6N2E2_35at 75.211 kb on + strand, within Pf6N2E2_35at 75.211 kb on + strand, within Pf6N2E2_35at 75.211 kb on + strand, within Pf6N2E2_35at 75.212 kb on - strand, within Pf6N2E2_35at 75.212 kb on - strand, within Pf6N2E2_35at 75.253 kb on + strand, within Pf6N2E2_35at 75.362 kb on + strand, within Pf6N2E2_35at 75.365 kb on - strand, within Pf6N2E2_35at 75.365 kb on - strand, within Pf6N2E2_35at 75.382 kb on + strand, within Pf6N2E2_35at 75.382 kb on + strand, within Pf6N2E2_35at 75.560 kb on - strandat 75.560 kb on - strandat 75.560 kb on - strandat 75.612 kb on + strandat 75.612 kb on + strandat 75.612 kb on + strandat 75.612 kb on + strandat 75.612 kb on + strandat 75.612 kb on + strandat 75.612 kb on + strandat 75.613 kb on - strandat 75.613 kb on - strandat 75.613 kb on - strandat 75.613 kb on - strandat 75.659 kb on + strandat 75.660 kb on - strandat 75.660 kb on - strandat 75.660 kb on - strandat 75.660 kb on - strandat 75.663 kb on + strandat 75.663 kb on + strandat 76.003 kb on + strand, within Pf6N2E2_36at 76.003 kb on + strand, within Pf6N2E2_36at 76.003 kb on + strand, within Pf6N2E2_36at 76.075 kb on + strand, within Pf6N2E2_36at 76.076 kb on - strand, within Pf6N2E2_36at 76.076 kb on - strand, within Pf6N2E2_36at 76.126 kb on + strand, within Pf6N2E2_36at 76.126 kb on + strand, within Pf6N2E2_36at 76.126 kb on + strand, within Pf6N2E2_36at 76.126 kb on + strand, within Pf6N2E2_36at 76.126 kb on + strand, within Pf6N2E2_36at 76.126 kb on + strand, within Pf6N2E2_36at 76.126 kb on + strand, within Pf6N2E2_36at 76.127 kb on - strand, within Pf6N2E2_36at 76.127 kb on - strand, within Pf6N2E2_36at 76.127 kb on - strand, within Pf6N2E2_36at 76.127 kb on - strand, within Pf6N2E2_36at 76.127 kb on - strand, within Pf6N2E2_36at 76.127 kb on - strand, within Pf6N2E2_36at 76.127 kb on - strand, within Pf6N2E2_36at 76.127 kb on - strand, within Pf6N2E2_36at 76.153 kb on + strand, within Pf6N2E2_36at 76.153 kb on + strand, within Pf6N2E2_36at 76.153 kb on + strand, within Pf6N2E2_36at 76.154 kb on - strand, within Pf6N2E2_36at 76.154 kb on - strand, within Pf6N2E2_36at 76.154 kb on - strand, within Pf6N2E2_36at 76.272 kb on + strand, within Pf6N2E2_36at 76.438 kb on + strand, within Pf6N2E2_36at 76.438 kb on + strand, within Pf6N2E2_36at 76.438 kb on + strand, within Pf6N2E2_36at 76.438 kb on + strand, within Pf6N2E2_36at 76.438 kb on + strand, within Pf6N2E2_36at 76.438 kb on + strand, within Pf6N2E2_36at 76.438 kb on + strand, within Pf6N2E2_36at 76.438 kb on + strand, within Pf6N2E2_36at 76.439 kb on - strand, within Pf6N2E2_36at 76.439 kb on - strand, within Pf6N2E2_36at 76.439 kb on - strand, within Pf6N2E2_36at 76.439 kb on - strand, within Pf6N2E2_36at 76.439 kb on - strand, within Pf6N2E2_36at 76.439 kb on - strand, within Pf6N2E2_36at 76.439 kb on - strand, within Pf6N2E2_36at 76.439 kb on - strand, within Pf6N2E2_36at 76.489 kb on + strand, within Pf6N2E2_36at 76.489 kb on + strand, within Pf6N2E2_36at 76.489 kb on + strand, within Pf6N2E2_36at 76.490 kb on - strand, within Pf6N2E2_36at 76.490 kb on - strand, within Pf6N2E2_36at 76.576 kb on + strandat 76.577 kb on - strandat 76.577 kb on - strandat 76.644 kb on + strandat 76.645 kb on - strandat 76.645 kb on - strandat 76.739 kb on + strandat 76.739 kb on + strandat 76.740 kb on - strandat 76.851 kb on + strandat 76.851 kb on + strandat 76.852 kb on - strandat 76.852 kb on - strandat 76.852 kb on - strandat 76.852 kb on - strandat 76.852 kb on - strandat 76.986 kb on + strand, within Pf6N2E2_37at 76.986 kb on + strand, within Pf6N2E2_37at 76.986 kb on + strand, within Pf6N2E2_37at 76.986 kb on + strand, within Pf6N2E2_37at 76.987 kb on - strand, within Pf6N2E2_37at 76.987 kb on - strand, within Pf6N2E2_37at 76.987 kb on - strand, within Pf6N2E2_37at 77.007 kb on + strand, within Pf6N2E2_37at 77.209 kb on + strandat 77.209 kb on + strandat 77.210 kb on - strandat 77.242 kb on + strandat 77.243 kb on - strandat 77.280 kb on - strandat 77.376 kb on - strandat 77.422 kb on - strand, within Pf6N2E2_38at 77.459 kb on + strand, within Pf6N2E2_38at 77.460 kb on - strand, within Pf6N2E2_38at 77.597 kb on + strand, within Pf6N2E2_38at 77.598 kb on - strand, within Pf6N2E2_38

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Leucine (C)
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74,821 + Pf6N2E2_35 0.40 +1.0
74,821 + Pf6N2E2_35 0.40 -0.1
74,821 + Pf6N2E2_35 0.40 +0.2
74,821 + Pf6N2E2_35 0.40 +0.5
74,821 + Pf6N2E2_35 0.40 -0.1
74,822 - Pf6N2E2_35 0.40 +0.0
75,107 - Pf6N2E2_35 0.62 +0.4
75,107 - Pf6N2E2_35 0.62 +1.2
75,139 + Pf6N2E2_35 0.65 +0.8
75,140 - Pf6N2E2_35 0.65 -2.0
75,140 - Pf6N2E2_35 0.65 -0.1
75,211 + Pf6N2E2_35 0.70 -1.1
75,211 + Pf6N2E2_35 0.70 +0.2
75,211 + Pf6N2E2_35 0.70 +0.5
75,212 - Pf6N2E2_35 0.70 -1.1
75,212 - Pf6N2E2_35 0.70 +0.0
75,253 + Pf6N2E2_35 0.74 -0.1
75,362 + Pf6N2E2_35 0.82 +0.3
75,365 - Pf6N2E2_35 0.82 +0.4
75,365 - Pf6N2E2_35 0.82 +0.8
75,382 + Pf6N2E2_35 0.84 +1.2
75,382 + Pf6N2E2_35 0.84 -2.2
75,560 - +1.5
75,560 - +0.1
75,560 - +1.0
75,612 + +0.7
75,612 + +0.4
75,612 + +0.7
75,612 + +0.6
75,612 + +0.6
75,612 + -1.1
75,612 + +0.1
75,613 - +0.2
75,613 - -0.6
75,613 - -1.8
75,613 - -0.1
75,659 + +0.1
75,660 - +1.2
75,660 - -0.3
75,660 - -0.7
75,660 - -0.8
75,663 + -1.0
75,663 + +1.0
76,003 + Pf6N2E2_36 0.28 +2.3
76,003 + Pf6N2E2_36 0.28 +0.2
76,003 + Pf6N2E2_36 0.28 +0.9
76,075 + Pf6N2E2_36 0.36 +0.4
76,076 - Pf6N2E2_36 0.36 -0.3
76,076 - Pf6N2E2_36 0.36 +0.3
76,126 + Pf6N2E2_36 0.42 -1.1
76,126 + Pf6N2E2_36 0.42 -1.5
76,126 + Pf6N2E2_36 0.42 +0.5
76,126 + Pf6N2E2_36 0.42 -1.1
76,126 + Pf6N2E2_36 0.42 +0.2
76,126 + Pf6N2E2_36 0.42 -0.5
76,126 + Pf6N2E2_36 0.42 +0.5
76,127 - Pf6N2E2_36 0.42 +0.9
76,127 - Pf6N2E2_36 0.42 -0.2
76,127 - Pf6N2E2_36 0.42 -2.2
76,127 - Pf6N2E2_36 0.42 -1.4
76,127 - Pf6N2E2_36 0.42 -1.1
76,127 - Pf6N2E2_36 0.42 +2.4
76,127 - Pf6N2E2_36 0.42 +2.2
76,127 - Pf6N2E2_36 0.42 +1.8
76,153 + Pf6N2E2_36 0.46 -2.0
76,153 + Pf6N2E2_36 0.46 -1.1
76,153 + Pf6N2E2_36 0.46 -1.0
76,154 - Pf6N2E2_36 0.46 -1.4
76,154 - Pf6N2E2_36 0.46 +0.7
76,154 - Pf6N2E2_36 0.46 +0.1
76,272 + Pf6N2E2_36 0.60 -1.6
76,438 + Pf6N2E2_36 0.80 +1.4
76,438 + Pf6N2E2_36 0.80 -0.4
76,438 + Pf6N2E2_36 0.80 -1.0
76,438 + Pf6N2E2_36 0.80 +0.3
76,438 + Pf6N2E2_36 0.80 -1.8
76,438 + Pf6N2E2_36 0.80 -0.5
76,438 + Pf6N2E2_36 0.80 -1.0
76,438 + Pf6N2E2_36 0.80 -2.8
76,439 - Pf6N2E2_36 0.80 -0.0
76,439 - Pf6N2E2_36 0.80 +0.5
76,439 - Pf6N2E2_36 0.80 +0.2
76,439 - Pf6N2E2_36 0.80 +0.3
76,439 - Pf6N2E2_36 0.80 +1.7
76,439 - Pf6N2E2_36 0.80 -1.6
76,439 - Pf6N2E2_36 0.80 +2.1
76,439 - Pf6N2E2_36 0.80 +1.0
76,489 + Pf6N2E2_36 0.86 -0.4
76,489 + Pf6N2E2_36 0.86 +0.5
76,489 + Pf6N2E2_36 0.86 +0.3
76,490 - Pf6N2E2_36 0.86 +0.7
76,490 - Pf6N2E2_36 0.86 +1.7
76,576 + +1.0
76,577 - -0.5
76,577 - -0.3
76,644 + -2.3
76,645 - +0.8
76,645 - -1.5
76,739 + +0.7
76,739 + +0.1
76,740 - -0.3
76,851 + +1.2
76,851 + -0.4
76,852 - +1.2
76,852 - +1.2
76,852 - +0.7
76,852 - +1.6
76,852 - +1.6
76,986 + Pf6N2E2_37 0.41 -3.3
76,986 + Pf6N2E2_37 0.41 -2.1
76,986 + Pf6N2E2_37 0.41 -1.6
76,986 + Pf6N2E2_37 0.41 -0.8
76,987 - Pf6N2E2_37 0.42 -0.8
76,987 - Pf6N2E2_37 0.42 -1.8
76,987 - Pf6N2E2_37 0.42 -2.0
77,007 + Pf6N2E2_37 0.47 -1.3
77,209 + -0.8
77,209 + +1.5
77,210 - -0.5
77,242 + +1.7
77,243 - +3.1
77,280 - +0.2
77,376 - +0.7
77,422 - Pf6N2E2_38 0.13 +1.0
77,459 + Pf6N2E2_38 0.17 +1.4
77,460 - Pf6N2E2_38 0.17 -0.1
77,597 + Pf6N2E2_38 0.30 -1.0
77,598 - Pf6N2E2_38 0.30 -0.8

Or see this region's nucleotide sequence