Experiment: PYE with CHIR-090 2 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02062 and CCNA_02063 are separated by 82 nucleotides CCNA_02063 and CCNA_02064 are separated by 100 nucleotides
CCNA_02062: CCNA_02062 - DNA repair protein recN, at 2,211,778 to 2,213,484
_02062
CCNA_02063: CCNA_02063 - beta-barrel assembly machine (BAM) protein BamD, at 2,213,567 to 2,214,496
_02063
CCNA_02064: CCNA_02064 - UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase, at 2,214,597 to 2,215,493
_02064
Position (kb)
2213
2214
2215 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2212.593 kb on - strand, within CCNA_02062 at 2212.680 kb on - strand, within CCNA_02062 at 2212.780 kb on - strand, within CCNA_02062 at 2212.786 kb on + strand, within CCNA_02062 at 2212.794 kb on - strand, within CCNA_02062 at 2212.794 kb on - strand, within CCNA_02062 at 2212.816 kb on - strand, within CCNA_02062 at 2212.839 kb on - strand, within CCNA_02062 at 2212.851 kb on - strand, within CCNA_02062 at 2212.868 kb on - strand, within CCNA_02062 at 2212.878 kb on + strand, within CCNA_02062 at 2212.878 kb on + strand, within CCNA_02062 at 2212.886 kb on - strand, within CCNA_02062 at 2212.886 kb on - strand, within CCNA_02062 at 2212.959 kb on + strand, within CCNA_02062 at 2212.967 kb on - strand, within CCNA_02062 at 2212.977 kb on - strand, within CCNA_02062 at 2213.062 kb on - strand, within CCNA_02062 at 2213.062 kb on - strand, within CCNA_02062 at 2213.078 kb on + strand, within CCNA_02062 at 2213.078 kb on + strand, within CCNA_02062 at 2213.078 kb on - strand, within CCNA_02062 at 2213.229 kb on + strand, within CCNA_02062 at 2213.304 kb on - strand, within CCNA_02062 at 2213.304 kb on - strand, within CCNA_02062 at 2213.494 kb on - strand at 2213.521 kb on + strand at 2213.567 kb on + strand at 2213.588 kb on - strand at 2213.594 kb on + strand at 2213.662 kb on - strand, within CCNA_02063 at 2213.672 kb on - strand, within CCNA_02063 at 2213.679 kb on - strand, within CCNA_02063 at 2213.690 kb on - strand, within CCNA_02063 at 2213.690 kb on - strand, within CCNA_02063 at 2213.771 kb on + strand, within CCNA_02063 at 2214.498 kb on - strand at 2214.523 kb on - strand at 2214.523 kb on - strand at 2214.525 kb on + strand at 2214.525 kb on + strand at 2214.533 kb on + strand at 2214.533 kb on - strand at 2214.533 kb on - strand at 2214.533 kb on - strand at 2214.539 kb on - strand at 2214.541 kb on - strand at 2214.541 kb on - strand at 2214.594 kb on + strand at 2214.602 kb on - strand at 2214.643 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 2 ug/mL remove 2,212,593 - CCNA_02062 0.48 +1.4 2,212,680 - CCNA_02062 0.53 +0.0 2,212,780 - CCNA_02062 0.59 +0.2 2,212,786 + CCNA_02062 0.59 -0.8 2,212,794 - CCNA_02062 0.60 +0.5 2,212,794 - CCNA_02062 0.60 +0.4 2,212,816 - CCNA_02062 0.61 +0.2 2,212,839 - CCNA_02062 0.62 -0.3 2,212,851 - CCNA_02062 0.63 -0.0 2,212,868 - CCNA_02062 0.64 -0.3 2,212,878 + CCNA_02062 0.64 +0.9 2,212,878 + CCNA_02062 0.64 -0.7 2,212,886 - CCNA_02062 0.65 -1.6 2,212,886 - CCNA_02062 0.65 +0.2 2,212,959 + CCNA_02062 0.69 +0.2 2,212,967 - CCNA_02062 0.70 -0.3 2,212,977 - CCNA_02062 0.70 +0.5 2,213,062 - CCNA_02062 0.75 -0.6 2,213,062 - CCNA_02062 0.75 +0.8 2,213,078 + CCNA_02062 0.76 -0.4 2,213,078 + CCNA_02062 0.76 +0.3 2,213,078 - CCNA_02062 0.76 +2.0 2,213,229 + CCNA_02062 0.85 -1.2 2,213,304 - CCNA_02062 0.89 +0.5 2,213,304 - CCNA_02062 0.89 -0.8 2,213,494 - +0.1 2,213,521 + -0.2 2,213,567 + +0.3 2,213,588 - +1.0 2,213,594 + -1.9 2,213,662 - CCNA_02063 0.10 +0.4 2,213,672 - CCNA_02063 0.11 -0.6 2,213,679 - CCNA_02063 0.12 +1.0 2,213,690 - CCNA_02063 0.13 +0.5 2,213,690 - CCNA_02063 0.13 +0.2 2,213,771 + CCNA_02063 0.22 +0.3 2,214,498 - +0.2 2,214,523 - -0.3 2,214,523 - +0.1 2,214,525 + -1.3 2,214,525 + -1.1 2,214,533 + -0.5 2,214,533 - +0.5 2,214,533 - +0.0 2,214,533 - +0.2 2,214,539 - +1.6 2,214,541 - -0.2 2,214,541 - +0.0 2,214,594 + +0.7 2,214,602 - +0.3 2,214,643 - +0.5
Or see this region's nucleotide sequence