Experiment: PYE with CHIR-090 2 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02039 and CCNA_02040 are separated by 335 nucleotides CCNA_02040 and CCNA_02041 are separated by 78 nucleotides CCNA_02041 and CCNA_02042 are separated by 168 nucleotides
CCNA_02039: CCNA_02039 - ATP-dependent endopeptidase clp, ATP-binding subunit ClpX, at 2,183,866 to 2,185,128
_02039
CCNA_02040: CCNA_02040 - phosphoserine phosphatase, at 2,185,464 to 2,186,132
_02040
CCNA_02041: CCNA_02041 - ATP-dependent endopeptidase clp, proteolytic subunit ClpP, at 2,186,211 to 2,186,852
_02041
CCNA_02042: CCNA_02042 - trigger factor chaperone, at 2,187,021 to 2,188,385
_02042
Position (kb)
2185
2186
2187 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2185.214 kb on - strand at 2185.260 kb on + strand at 2185.301 kb on + strand at 2185.316 kb on + strand at 2185.325 kb on - strand at 2185.371 kb on + strand at 2185.384 kb on - strand at 2185.394 kb on - strand at 2185.394 kb on - strand at 2185.431 kb on - strand at 2185.438 kb on + strand at 2185.472 kb on + strand at 2185.484 kb on + strand at 2185.485 kb on + strand at 2185.491 kb on + strand at 2185.541 kb on + strand, within CCNA_02040 at 2186.134 kb on - strand at 2186.173 kb on + strand at 2186.181 kb on - strand at 2186.187 kb on + strand at 2186.208 kb on + strand at 2186.208 kb on + strand at 2186.216 kb on - strand at 2186.216 kb on - strand at 2186.216 kb on - strand at 2186.234 kb on + strand at 2186.234 kb on + strand at 2186.923 kb on + strand at 2186.923 kb on + strand at 2186.923 kb on + strand at 2186.931 kb on - strand at 2186.942 kb on - strand at 2187.099 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 2 ug/mL remove 2,185,214 - +0.3 2,185,260 + -0.3 2,185,301 + +0.5 2,185,316 + +0.1 2,185,325 - +1.0 2,185,371 + -0.3 2,185,384 - +0.2 2,185,394 - -1.2 2,185,394 - +1.1 2,185,431 - +0.4 2,185,438 + -0.6 2,185,472 + +0.4 2,185,484 + +0.3 2,185,485 + -0.6 2,185,491 + -0.7 2,185,541 + CCNA_02040 0.12 +0.4 2,186,134 - +1.6 2,186,173 + +0.5 2,186,181 - -0.3 2,186,187 + +0.8 2,186,208 + +0.8 2,186,208 + -1.4 2,186,216 - +1.1 2,186,216 - +0.4 2,186,216 - +1.8 2,186,234 + -1.7 2,186,234 + -2.0 2,186,923 + +0.7 2,186,923 + -0.6 2,186,923 + -0.5 2,186,931 - -2.2 2,186,942 - +1.2 2,187,099 - +0.1
Or see this region's nucleotide sequence