Experiment: PYE with CHIR-090 2 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01937 and CCNA_01938 overlap by 4 nucleotides CCNA_01938 and CCNA_01939 overlap by 8 nucleotides CCNA_01939 and CCNA_01940 overlap by 4 nucleotides
CCNA_01937: CCNA_01937 - FeS assembly protein SufD, at 2,080,163 to 2,081,191
_01937
CCNA_01938: CCNA_01938 - ATP-dependent transporter SufC, at 2,081,188 to 2,081,937
_01938
CCNA_01939: CCNA_01939 - ADP-ribosylglycohydrolase, at 2,081,930 to 2,082,319
_01939
CCNA_01940: CCNA_01940 - ABC transporter-associated protein SufB, at 2,082,316 to 2,083,785
_01940
Position (kb)
2081
2082
2083 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2081.099 kb on - strand at 2081.174 kb on - strand at 2081.974 kb on - strand, within CCNA_01939 at 2082.010 kb on - strand, within CCNA_01939 at 2082.045 kb on + strand, within CCNA_01939 at 2082.045 kb on + strand, within CCNA_01939 at 2082.053 kb on - strand, within CCNA_01939 at 2082.087 kb on - strand, within CCNA_01939 at 2082.191 kb on + strand, within CCNA_01939 at 2082.216 kb on - strand, within CCNA_01939 at 2082.257 kb on - strand, within CCNA_01939 at 2082.288 kb on - strand at 2082.302 kb on - strand at 2082.302 kb on - strand at 2082.304 kb on + strand at 2082.304 kb on + strand at 2082.306 kb on - strand at 2082.312 kb on - strand at 2082.312 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 2 ug/mL remove 2,081,099 - -4.0 2,081,174 - +0.4 2,081,974 - CCNA_01939 0.11 +0.1 2,082,010 - CCNA_01939 0.21 +1.1 2,082,045 + CCNA_01939 0.29 -0.8 2,082,045 + CCNA_01939 0.29 +0.1 2,082,053 - CCNA_01939 0.32 +1.1 2,082,087 - CCNA_01939 0.40 +0.7 2,082,191 + CCNA_01939 0.67 +1.3 2,082,216 - CCNA_01939 0.73 +0.6 2,082,257 - CCNA_01939 0.84 +1.1 2,082,288 - +0.2 2,082,302 - -0.1 2,082,302 - +1.4 2,082,304 + +0.0 2,082,304 + -1.6 2,082,306 - +0.6 2,082,312 - +0.3 2,082,312 - +1.7
Or see this region's nucleotide sequence