Experiment: PYE with CHIR-090 2 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01800 and CCNA_01801 are separated by 5 nucleotides CCNA_01801 and CCNA_01802 overlap by 4 nucleotides CCNA_01802 and CCNA_01803 are separated by 12 nucleotides
CCNA_01800: CCNA_01800 - pyruvate dehydrogenase E1 component beta subunit, at 1,924,985 to 1,926,337
_01800
CCNA_01801: CCNA_01801 - hypothetical protein, at 1,926,343 to 1,926,795
_01801
CCNA_01802: CCNA_01802 - hypothetical protein, at 1,926,792 to 1,926,908
_01802
CCNA_01803: CCNA_01803 - pyruvate dehydrogenase complex, dihydrolipoamide acetyltransferase component, at 1,926,921 to 1,928,207
_01803
Position (kb) 1926
1927 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 1926.435 kb on + strand, within CCNA_01801 at 1926.613 kb on - strand, within CCNA_01801 at 1926.626 kb on + strand, within CCNA_01801 at 1926.631 kb on + strand, within CCNA_01801 at 1926.631 kb on + strand at 1926.631 kb on + strand, within CCNA_01801 at 1926.631 kb on + strand, within CCNA_01801 at 1926.656 kb on + strand, within CCNA_01801 at 1926.689 kb on - strand, within CCNA_01801 at 1926.694 kb on + strand, within CCNA_01801 at 1926.699 kb on + strand, within CCNA_01801 at 1926.704 kb on + strand, within CCNA_01801 at 1926.733 kb on - strand, within CCNA_01801 at 1926.738 kb on + strand, within CCNA_01801 at 1926.739 kb on + strand, within CCNA_01801 at 1926.771 kb on + strand at 1926.771 kb on + strand at 1926.779 kb on - strand at 1926.801 kb on + strand at 1926.819 kb on - strand, within CCNA_01802 at 1926.819 kb on - strand, within CCNA_01802 at 1926.819 kb on - strand, within CCNA_01802 at 1926.838 kb on + strand, within CCNA_01802 at 1926.838 kb on + strand, within CCNA_01802 at 1926.884 kb on + strand, within CCNA_01802
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 2 ug/mL remove 1,926,435 + CCNA_01801 0.20 +1.5 1,926,613 - CCNA_01801 0.60 -1.1 1,926,626 + CCNA_01801 0.62 +3.0 1,926,631 + CCNA_01801 0.64 +0.4 1,926,631 + +4.6 1,926,631 + CCNA_01801 0.64 +0.7 1,926,631 + CCNA_01801 0.64 +1.0 1,926,656 + CCNA_01801 0.69 +1.3 1,926,689 - CCNA_01801 0.76 +0.6 1,926,694 + CCNA_01801 0.77 +1.1 1,926,699 + CCNA_01801 0.79 +0.0 1,926,704 + CCNA_01801 0.80 -0.1 1,926,733 - CCNA_01801 0.86 +0.1 1,926,738 + CCNA_01801 0.87 +0.0 1,926,739 + CCNA_01801 0.87 +0.9 1,926,771 + +0.7 1,926,771 + +0.6 1,926,779 - +2.4 1,926,801 + +1.1 1,926,819 - CCNA_01802 0.23 +0.7 1,926,819 - CCNA_01802 0.23 -2.4 1,926,819 - CCNA_01802 0.23 -2.6 1,926,838 + CCNA_01802 0.39 +0.6 1,926,838 + CCNA_01802 0.39 +0.8 1,926,884 + CCNA_01802 0.79 +0.4
Or see this region's nucleotide sequence