Experiment: PYE with CHIR-090 2 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00892 and CCNA_00893 are separated by 159 nucleotides CCNA_00893 and CCNA_00894 are separated by 181 nucleotides
CCNA_00892: CCNA_00892 - phosphoenolpyruvate-protein phosphotransferase PtsP, at 970,314 to 972,578
_00892
CCNA_00893: CCNA_00893 - helix-turn-helix DNA-binding protein, at 972,738 to 973,802
_00893
CCNA_00894: CCNA_00894 - 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase, at 973,984 to 975,135
_00894
Position (kb) 972
973
974 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 971.937 kb on + strand, within CCNA_00892 at 972.506 kb on + strand at 972.514 kb on - strand at 972.686 kb on - strand at 972.793 kb on - strand at 972.801 kb on + strand at 972.841 kb on - strand at 972.857 kb on + strand, within CCNA_00893 at 973.453 kb on + strand, within CCNA_00893 at 973.453 kb on + strand, within CCNA_00893 at 973.481 kb on + strand, within CCNA_00893 at 973.669 kb on + strand, within CCNA_00893 at 973.677 kb on - strand, within CCNA_00893 at 973.739 kb on + strand at 973.739 kb on + strand at 973.744 kb on + strand at 973.748 kb on + strand at 973.748 kb on + strand at 973.756 kb on - strand at 973.806 kb on + strand at 973.814 kb on - strand at 973.828 kb on - strand at 973.846 kb on + strand at 973.876 kb on - strand at 973.942 kb on - strand at 973.942 kb on - strand at 973.948 kb on + strand at 973.948 kb on + strand at 973.956 kb on - strand at 973.959 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 2 ug/mL remove 971,937 + CCNA_00892 0.72 -0.0 972,506 + +2.8 972,514 - +0.8 972,686 - -0.6 972,793 - -1.9 972,801 + -0.6 972,841 - -1.5 972,857 + CCNA_00893 0.11 -0.8 973,453 + CCNA_00893 0.67 -0.4 973,453 + CCNA_00893 0.67 -2.1 973,481 + CCNA_00893 0.70 -0.1 973,669 + CCNA_00893 0.87 -1.4 973,677 - CCNA_00893 0.88 +1.8 973,739 + +2.8 973,739 + -0.3 973,744 + +0.6 973,748 + +0.6 973,748 + +0.9 973,756 - -2.2 973,806 + +0.6 973,814 - +0.2 973,828 - -0.5 973,846 + +1.4 973,876 - -0.1 973,942 - +1.1 973,942 - -0.3 973,948 + +0.1 973,948 + +0.0 973,956 - +0.4 973,959 + +0.8
Or see this region's nucleotide sequence