Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25305

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.33x; and D-Glucose 74 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25300 and BWI76_RS25305 are separated by 142 nucleotidesBWI76_RS25305 and BWI76_RS25310 are separated by 60 nucleotidesBWI76_RS25310 and BWI76_RS25315 are separated by 415 nucleotides BWI76_RS25300: BWI76_RS25300 - hybrid sensor histidine kinase/response regulator, at 5,256,977 to 5,259,316 _RS25300 BWI76_RS25305: BWI76_RS25305 - TIGR01212 family radical SAM protein, at 5,259,459 to 5,260,394 _RS25305 BWI76_RS25310: BWI76_RS25310 - hypothetical protein, at 5,260,455 to 5,260,652 _RS25310 BWI76_RS25315: BWI76_RS25315 - glutamate synthase large subunit, at 5,261,068 to 5,265,528 _RS25315 Position (kb) 5259 5260 5261Strain fitness (log2 ratio) -2 -1 0 1 2at 5258.587 kb on + strand, within BWI76_RS25300at 5258.761 kb on + strand, within BWI76_RS25300at 5258.990 kb on - strand, within BWI76_RS25300at 5259.039 kb on - strand, within BWI76_RS25300at 5259.105 kb on - strandat 5259.170 kb on - strandat 5259.189 kb on - strandat 5259.282 kb on + strandat 5259.290 kb on - strandat 5259.290 kb on - strandat 5259.416 kb on + strandat 5259.439 kb on + strandat 5259.512 kb on - strandat 5259.568 kb on + strand, within BWI76_RS25305at 5259.571 kb on - strand, within BWI76_RS25305at 5259.718 kb on - strand, within BWI76_RS25305at 5259.758 kb on + strand, within BWI76_RS25305at 5259.766 kb on - strand, within BWI76_RS25305at 5259.937 kb on + strand, within BWI76_RS25305at 5259.969 kb on + strand, within BWI76_RS25305at 5259.985 kb on + strand, within BWI76_RS25305at 5260.709 kb on - strandat 5260.709 kb on - strandat 5260.716 kb on + strandat 5260.718 kb on - strandat 5260.824 kb on - strandat 5260.855 kb on + strandat 5260.866 kb on + strandat 5260.981 kb on + strandat 5260.995 kb on + strandat 5261.038 kb on + strandat 5261.042 kb on + strandat 5261.047 kb on + strandat 5261.085 kb on - strandat 5261.161 kb on + strandat 5261.183 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.33x; and D-Glucose 74 mM
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5,258,587 + BWI76_RS25300 0.69 -1.6
5,258,761 + BWI76_RS25300 0.76 -1.4
5,258,990 - BWI76_RS25300 0.86 -0.7
5,259,039 - BWI76_RS25300 0.88 +0.3
5,259,105 - -0.1
5,259,170 - -0.2
5,259,189 - +0.1
5,259,282 + -0.2
5,259,290 - -0.3
5,259,290 - -1.0
5,259,416 + -0.3
5,259,439 + +0.3
5,259,512 - +0.7
5,259,568 + BWI76_RS25305 0.12 -0.1
5,259,571 - BWI76_RS25305 0.12 -0.3
5,259,718 - BWI76_RS25305 0.28 +1.0
5,259,758 + BWI76_RS25305 0.32 +2.4
5,259,766 - BWI76_RS25305 0.33 +0.0
5,259,937 + BWI76_RS25305 0.51 +0.4
5,259,969 + BWI76_RS25305 0.54 +0.1
5,259,985 + BWI76_RS25305 0.56 +0.6
5,260,709 - -1.2
5,260,709 - -2.4
5,260,716 + -0.3
5,260,718 - -1.4
5,260,824 - -0.1
5,260,855 + +0.2
5,260,866 + -1.5
5,260,981 + -1.6
5,260,995 + -0.0
5,261,038 + -0.3
5,261,042 + -0.1
5,261,047 + -0.3
5,261,085 - -2.5
5,261,161 + -1.8
5,261,183 - -1.9

Or see this region's nucleotide sequence