Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25230

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.33x; and D-Glucose 74 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25220 and BWI76_RS25225 are separated by 4 nucleotidesBWI76_RS25225 and BWI76_RS25230 are separated by 7 nucleotidesBWI76_RS25230 and BWI76_RS25235 are separated by 209 nucleotides BWI76_RS25220: BWI76_RS25220 - outer membrane lipid asymmetry maintenance protein MlaD, at 5,245,054 to 5,245,605 _RS25220 BWI76_RS25225: BWI76_RS25225 - ABC transporter permease, at 5,245,610 to 5,246,392 _RS25225 BWI76_RS25230: BWI76_RS25230 - phospholipid ABC transporter ATP-binding protein MlaF, at 5,246,400 to 5,247,212 _RS25230 BWI76_RS25235: BWI76_RS25235 - calcium/sodium antiporter, at 5,247,422 to 5,248,399 _RS25235 Position (kb) 5246 5247 5248Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 5245.572 kb on + strandat 5246.024 kb on + strand, within BWI76_RS25225at 5246.161 kb on + strand, within BWI76_RS25225at 5246.241 kb on + strand, within BWI76_RS25225at 5246.241 kb on + strand, within BWI76_RS25225at 5246.252 kb on - strand, within BWI76_RS25225at 5246.294 kb on + strand, within BWI76_RS25225at 5246.338 kb on - strandat 5246.538 kb on - strand, within BWI76_RS25230at 5246.629 kb on + strand, within BWI76_RS25230at 5246.740 kb on + strand, within BWI76_RS25230at 5246.845 kb on - strand, within BWI76_RS25230at 5246.909 kb on + strand, within BWI76_RS25230at 5246.947 kb on - strand, within BWI76_RS25230at 5246.968 kb on + strand, within BWI76_RS25230at 5246.976 kb on - strand, within BWI76_RS25230at 5246.976 kb on - strand, within BWI76_RS25230at 5246.976 kb on - strand, within BWI76_RS25230at 5247.247 kb on - strandat 5247.379 kb on - strandat 5247.432 kb on - strandat 5247.487 kb on + strandat 5247.487 kb on + strandat 5247.487 kb on + strandat 5247.487 kb on + strandat 5247.553 kb on + strand, within BWI76_RS25235at 5247.605 kb on - strand, within BWI76_RS25235at 5247.633 kb on + strand, within BWI76_RS25235at 5247.699 kb on - strand, within BWI76_RS25235at 5247.883 kb on - strand, within BWI76_RS25235at 5247.883 kb on - strandat 5247.998 kb on + strand, within BWI76_RS25235at 5248.166 kb on + strand, within BWI76_RS25235

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.33x; and D-Glucose 74 mM
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5,245,572 + -3.3
5,246,024 + BWI76_RS25225 0.53 -3.1
5,246,161 + BWI76_RS25225 0.70 -2.0
5,246,241 + BWI76_RS25225 0.81 -4.8
5,246,241 + BWI76_RS25225 0.81 -2.3
5,246,252 - BWI76_RS25225 0.82 -5.0
5,246,294 + BWI76_RS25225 0.87 -3.0
5,246,338 - -3.4
5,246,538 - BWI76_RS25230 0.17 -1.9
5,246,629 + BWI76_RS25230 0.28 -3.9
5,246,740 + BWI76_RS25230 0.42 -4.6
5,246,845 - BWI76_RS25230 0.55 -1.3
5,246,909 + BWI76_RS25230 0.63 -3.6
5,246,947 - BWI76_RS25230 0.67 -4.2
5,246,968 + BWI76_RS25230 0.70 -3.3
5,246,976 - BWI76_RS25230 0.71 -3.5
5,246,976 - BWI76_RS25230 0.71 -2.3
5,246,976 - BWI76_RS25230 0.71 -6.0
5,247,247 - -0.0
5,247,379 - -0.4
5,247,432 - -0.4
5,247,487 + +0.1
5,247,487 + -0.9
5,247,487 + -0.7
5,247,487 + -0.3
5,247,553 + BWI76_RS25235 0.13 +0.0
5,247,605 - BWI76_RS25235 0.19 -0.1
5,247,633 + BWI76_RS25235 0.22 -0.0
5,247,699 - BWI76_RS25235 0.28 -0.7
5,247,883 - BWI76_RS25235 0.47 -0.6
5,247,883 - +1.0
5,247,998 + BWI76_RS25235 0.59 +0.3
5,248,166 + BWI76_RS25235 0.76 -0.0

Or see this region's nucleotide sequence