Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.33x; and D-Glucose 74 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20640 and BWI76_RS20645 are separated by 381 nucleotides BWI76_RS20645 and BWI76_RS20650 are separated by 44 nucleotides BWI76_RS20650 and BWI76_RS20655 are separated by 42 nucleotides BWI76_RS20655 and BWI76_RS20660 are separated by 42 nucleotides BWI76_RS20660 and BWI76_RS20665 overlap by 4 nucleotides
BWI76_RS20640: BWI76_RS20640 - cysteine synthase A, at 4,319,208 to 4,320,179
_RS20640
BWI76_RS20645: BWI76_RS20645 - phosphocarrier protein HPr, at 4,320,561 to 4,320,818
_RS20645
BWI76_RS20650: BWI76_RS20650 - phosphoenolpyruvate--protein phosphotransferase, at 4,320,863 to 4,322,590
_RS20650
BWI76_RS20655: BWI76_RS20655 - glucose-specific phosphotransferase enzyme IIA component, at 4,322,633 to 4,323,142
_RS20655
BWI76_RS20660: BWI76_RS20660 - hypothetical protein, at 4,323,185 to 4,323,424
_RS20660
BWI76_RS20665: BWI76_RS20665 - bifunctional pyridoxal kinase/hydroxymethylpyrimidine kinase, at 4,323,421 to 4,324,287
_RS20665
Position (kb) 4320
4321
4322
4323 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4319.865 kb on - strand, within BWI76_RS20640 at 4320.263 kb on - strand at 4320.449 kb on + strand at 4320.786 kb on - strand, within BWI76_RS20645 at 4321.146 kb on - strand, within BWI76_RS20650 at 4321.357 kb on + strand, within BWI76_RS20650 at 4321.684 kb on + strand, within BWI76_RS20650 at 4322.527 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.33x; and D-Glucose 74 mM remove 4,319,865 - BWI76_RS20640 0.68 +1.1 4,320,263 - -1.3 4,320,449 + -1.3 4,320,786 - BWI76_RS20645 0.87 +1.1 4,321,146 - BWI76_RS20650 0.16 -0.5 4,321,357 + BWI76_RS20650 0.29 -1.0 4,321,684 + BWI76_RS20650 0.48 -3.2 4,322,527 + -2.4
Or see this region's nucleotide sequence