Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28020

Experiment: Phenylacetic 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28015 and BPHYT_RS28020 overlap by 4 nucleotidesBPHYT_RS28020 and BPHYT_RS28025 overlap by 4 nucleotidesBPHYT_RS28025 and BPHYT_RS28030 are separated by 24 nucleotides BPHYT_RS28015: BPHYT_RS28015 - enoyl-CoA hydratase, at 1,848,491 to 1,849,639 _RS28015 BPHYT_RS28020: BPHYT_RS28020 - enoyl-CoA hydratase, at 1,849,636 to 1,850,454 _RS28020 BPHYT_RS28025: BPHYT_RS28025 - 3-hydroxyisobutyrate dehydrogenase, at 1,850,451 to 1,851,365 _RS28025 BPHYT_RS28030: BPHYT_RS28030 - methylmalonate-semialdehyde dehydrogenase, at 1,851,390 to 1,852,901 _RS28030 Position (kb) 1849 1850 1851Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1848.756 kb on + strand, within BPHYT_RS28015at 1848.793 kb on - strand, within BPHYT_RS28015at 1848.793 kb on - strand, within BPHYT_RS28015at 1849.117 kb on - strand, within BPHYT_RS28015at 1850.438 kb on + strandat 1850.713 kb on + strand, within BPHYT_RS28025at 1850.938 kb on + strand, within BPHYT_RS28025at 1851.282 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Phenylacetic 5 mM (C)
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1,848,756 + BPHYT_RS28015 0.23 -0.4
1,848,793 - BPHYT_RS28015 0.26 -0.3
1,848,793 - BPHYT_RS28015 0.26 -3.1
1,849,117 - BPHYT_RS28015 0.54 +0.4
1,850,438 + +0.3
1,850,713 + BPHYT_RS28025 0.29 +2.2
1,850,938 + BPHYT_RS28025 0.53 -0.9
1,851,282 + -0.1

Or see this region's nucleotide sequence