Experiment: Varel_Bryant_medium_Glucose with Cefoxitin 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0046 and BT0047 are separated by 14 nucleotides BT0047 and BT0048 are separated by 95 nucleotides BT0048 and BT0049 are separated by 8 nucleotides BT0049 and BT0050 are separated by 11 nucleotides BT0050 and BT0051 overlap by 1 nucleotides
BT0046: BT0046 - putative glycosyltransferase (NCBI ptt file), at 42,724 to 43,656
BT0046
BT0047: BT0047 - putative glycosyltransferase (NCBI ptt file), at 43,671 to 44,066
BT0047
BT0048: BT0048 - hypothetical protein (NCBI ptt file), at 44,162 to 44,638
BT0048
BT0049: BT0049 - putative glycosyltransferase (NCBI ptt file), at 44,647 to 45,582
BT0049
BT0050: BT0050 - putative glycosyltransferase (NCBI ptt file), at 45,594 to 46,565
BT0050
BT0051: BT0051 - putative glycosyltransferase (NCBI ptt file), at 46,565 to 47,545
BT0051
Position (kb) 44
45
46 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 43.681 kb on + strand at 43.701 kb on + strand at 43.755 kb on - strand, within BT0047 at 43.774 kb on + strand, within BT0047 at 43.843 kb on - strand, within BT0047 at 43.858 kb on - strand, within BT0047 at 43.899 kb on + strand, within BT0047 at 43.899 kb on + strand, within BT0047 at 43.899 kb on + strand, within BT0047 at 43.957 kb on - strand, within BT0047 at 44.167 kb on + strand at 44.180 kb on - strand at 44.266 kb on - strand, within BT0048 at 44.315 kb on - strand, within BT0048 at 44.353 kb on - strand, within BT0048 at 44.376 kb on - strand, within BT0048 at 44.376 kb on - strand, within BT0048 at 44.409 kb on + strand, within BT0048 at 44.468 kb on + strand, within BT0048 at 44.510 kb on - strand, within BT0048 at 44.600 kb on - strand at 44.782 kb on + strand, within BT0049 at 44.784 kb on + strand, within BT0049 at 44.893 kb on + strand, within BT0049 at 44.978 kb on - strand, within BT0049 at 44.978 kb on - strand, within BT0049 at 44.978 kb on - strand, within BT0049 at 45.042 kb on - strand, within BT0049 at 45.089 kb on - strand, within BT0049 at 45.119 kb on + strand, within BT0049 at 45.155 kb on + strand, within BT0049 at 45.201 kb on - strand, within BT0049 at 45.259 kb on - strand, within BT0049 at 45.270 kb on - strand, within BT0049 at 45.301 kb on + strand, within BT0049 at 45.337 kb on - strand, within BT0049 at 45.370 kb on - strand, within BT0049 at 45.423 kb on + strand, within BT0049 at 45.423 kb on + strand, within BT0049 at 45.550 kb on + strand at 45.567 kb on - strand at 45.630 kb on + strand at 45.661 kb on + strand at 45.703 kb on + strand, within BT0050 at 45.731 kb on + strand, within BT0050 at 45.742 kb on + strand, within BT0050 at 45.743 kb on - strand, within BT0050 at 45.943 kb on - strand, within BT0050 at 45.981 kb on + strand, within BT0050 at 46.014 kb on - strand, within BT0050 at 46.162 kb on - strand, within BT0050 at 46.213 kb on - strand, within BT0050 at 46.218 kb on - strand, within BT0050 at 46.364 kb on + strand, within BT0050 at 46.364 kb on + strand, within BT0050 at 46.365 kb on - strand, within BT0050 at 46.365 kb on - strand, within BT0050 at 46.366 kb on + strand, within BT0050 at 46.367 kb on - strand, within BT0050 at 46.382 kb on + strand, within BT0050 at 46.459 kb on - strand, within BT0050 at 46.484 kb on + strand at 46.558 kb on - strand at 46.560 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Cefoxitin 0.0125 mM remove 43,681 + -2.9 43,701 + -0.6 43,755 - BT0047 0.21 +0.5 43,774 + BT0047 0.26 -2.4 43,843 - BT0047 0.43 -1.0 43,858 - BT0047 0.47 -0.9 43,899 + BT0047 0.58 -2.6 43,899 + BT0047 0.58 -0.0 43,899 + BT0047 0.58 -0.7 43,957 - BT0047 0.72 -0.7 44,167 + -0.0 44,180 - -0.0 44,266 - BT0048 0.22 -2.4 44,315 - BT0048 0.32 +1.5 44,353 - BT0048 0.40 -1.9 44,376 - BT0048 0.45 -1.6 44,376 - BT0048 0.45 -1.5 44,409 + BT0048 0.52 -1.4 44,468 + BT0048 0.64 -0.0 44,510 - BT0048 0.73 -0.6 44,600 - -1.3 44,782 + BT0049 0.14 -1.0 44,784 + BT0049 0.15 -1.0 44,893 + BT0049 0.26 -1.0 44,978 - BT0049 0.35 -1.5 44,978 - BT0049 0.35 -3.0 44,978 - BT0049 0.35 -2.2 45,042 - BT0049 0.42 -2.4 45,089 - BT0049 0.47 -1.1 45,119 + BT0049 0.50 -2.4 45,155 + BT0049 0.54 -3.0 45,201 - BT0049 0.59 +2.3 45,259 - BT0049 0.65 -2.9 45,270 - BT0049 0.67 -2.0 45,301 + BT0049 0.70 -1.6 45,337 - BT0049 0.74 -1.0 45,370 - BT0049 0.77 -1.0 45,423 + BT0049 0.83 -1.0 45,423 + BT0049 0.83 -1.0 45,550 + -0.0 45,567 - +0.1 45,630 + -2.4 45,661 + -1.3 45,703 + BT0050 0.11 -0.5 45,731 + BT0050 0.14 -0.0 45,742 + BT0050 0.15 -1.0 45,743 - BT0050 0.15 -1.1 45,943 - BT0050 0.36 -0.0 45,981 + BT0050 0.40 +0.7 46,014 - BT0050 0.43 -1.6 46,162 - BT0050 0.58 -2.4 46,213 - BT0050 0.64 -1.6 46,218 - BT0050 0.64 -0.8 46,364 + BT0050 0.79 -2.4 46,364 + BT0050 0.79 +0.3 46,365 - BT0050 0.79 -1.8 46,365 - BT0050 0.79 -1.0 46,366 + BT0050 0.79 -0.2 46,367 - BT0050 0.80 -3.5 46,382 + BT0050 0.81 -3.1 46,459 - BT0050 0.89 -0.8 46,484 + -1.3 46,558 - -0.0 46,560 + -1.0
Or see this region's nucleotide sequence