Experiment: D-Trehalose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1861 and Pf6N2E2_1862 are separated by 334 nucleotides Pf6N2E2_1862 and Pf6N2E2_1863 are separated by 0 nucleotides Pf6N2E2_1863 and Pf6N2E2_1864 are separated by 416 nucleotides
Pf6N2E2_1861 - Ferredoxin reductase, at 2,204,360 to 2,205,595
_1861
Pf6N2E2_1862 - Benzoate 1,2-dioxygenase beta subunit (EC 1.14.12.10), at 2,205,930 to 2,206,424
_1862
Pf6N2E2_1863 - putative dioxygenase hydroxylase component, at 2,206,425 to 2,207,684
_1863
Pf6N2E2_1864 - Transcriptional regulator, TetR family, at 2,208,101 to 2,208,751
_1864
Position (kb)
2206
2207
2208 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2205.438 kb on + strand, within Pf6N2E2_1861 at 2205.439 kb on - strand, within Pf6N2E2_1861 at 2205.591 kb on + strand at 2205.592 kb on - strand at 2205.619 kb on + strand at 2205.620 kb on - strand at 2205.701 kb on + strand at 2205.701 kb on + strand at 2205.701 kb on + strand at 2205.702 kb on - strand at 2206.031 kb on - strand, within Pf6N2E2_1862 at 2206.092 kb on - strand, within Pf6N2E2_1862 at 2206.092 kb on - strand, within Pf6N2E2_1862 at 2206.194 kb on - strand, within Pf6N2E2_1862 at 2206.237 kb on + strand, within Pf6N2E2_1862 at 2206.237 kb on + strand, within Pf6N2E2_1862 at 2206.241 kb on + strand, within Pf6N2E2_1862 at 2206.295 kb on - strand, within Pf6N2E2_1862 at 2206.343 kb on + strand, within Pf6N2E2_1862 at 2206.427 kb on - strand at 2206.550 kb on - strand at 2206.597 kb on + strand, within Pf6N2E2_1863 at 2206.840 kb on + strand, within Pf6N2E2_1863 at 2206.841 kb on - strand, within Pf6N2E2_1863 at 2206.865 kb on - strand, within Pf6N2E2_1863 at 2206.996 kb on + strand, within Pf6N2E2_1863 at 2206.996 kb on + strand, within Pf6N2E2_1863 at 2206.997 kb on - strand, within Pf6N2E2_1863 at 2206.997 kb on - strand, within Pf6N2E2_1863 at 2206.997 kb on - strand, within Pf6N2E2_1863 at 2206.997 kb on - strand, within Pf6N2E2_1863 at 2206.997 kb on - strand, within Pf6N2E2_1863 at 2207.251 kb on + strand, within Pf6N2E2_1863 at 2207.369 kb on - strand, within Pf6N2E2_1863 at 2207.379 kb on - strand, within Pf6N2E2_1863 at 2207.379 kb on - strand, within Pf6N2E2_1863 at 2207.811 kb on - strand at 2207.850 kb on - strand at 2207.850 kb on - strand at 2207.988 kb on + strand at 2207.989 kb on - strand at 2207.989 kb on - strand at 2207.989 kb on - strand at 2208.043 kb on + strand at 2208.116 kb on + strand at 2208.176 kb on + strand, within Pf6N2E2_1864 at 2208.190 kb on + strand, within Pf6N2E2_1864 at 2208.191 kb on - strand, within Pf6N2E2_1864 at 2208.191 kb on - strand, within Pf6N2E2_1864 at 2208.269 kb on - strand, within Pf6N2E2_1864 at 2208.315 kb on + strand, within Pf6N2E2_1864 at 2208.564 kb on + strand, within Pf6N2E2_1864 at 2208.564 kb on + strand, within Pf6N2E2_1864 at 2208.565 kb on - strand, within Pf6N2E2_1864 at 2208.569 kb on - strand, within Pf6N2E2_1864 at 2208.569 kb on - strand, within Pf6N2E2_1864 at 2208.569 kb on - strand, within Pf6N2E2_1864 at 2208.569 kb on - strand, within Pf6N2E2_1864 at 2208.579 kb on + strand, within Pf6N2E2_1864
Per-strain Table
Position Strand Gene LocusTag Fraction D-Trehalose (C) remove 2,205,438 + Pf6N2E2_1861 0.87 +0.4 2,205,439 - Pf6N2E2_1861 0.87 +0.3 2,205,591 + +0.7 2,205,592 - -0.7 2,205,619 + +0.8 2,205,620 - +1.6 2,205,701 + -0.8 2,205,701 + +1.2 2,205,701 + -0.4 2,205,702 - -1.4 2,206,031 - Pf6N2E2_1862 0.20 -1.3 2,206,092 - Pf6N2E2_1862 0.33 +1.5 2,206,092 - Pf6N2E2_1862 0.33 +0.8 2,206,194 - Pf6N2E2_1862 0.53 +2.2 2,206,237 + Pf6N2E2_1862 0.62 -0.0 2,206,237 + Pf6N2E2_1862 0.62 +0.9 2,206,241 + Pf6N2E2_1862 0.63 +0.5 2,206,295 - Pf6N2E2_1862 0.74 +0.2 2,206,343 + Pf6N2E2_1862 0.83 -0.8 2,206,427 - +0.2 2,206,550 - +0.4 2,206,597 + Pf6N2E2_1863 0.14 -0.2 2,206,840 + Pf6N2E2_1863 0.33 +1.4 2,206,841 - Pf6N2E2_1863 0.33 +0.6 2,206,865 - Pf6N2E2_1863 0.35 +2.1 2,206,996 + Pf6N2E2_1863 0.45 +1.2 2,206,996 + Pf6N2E2_1863 0.45 -0.5 2,206,997 - Pf6N2E2_1863 0.45 +0.9 2,206,997 - Pf6N2E2_1863 0.45 -1.8 2,206,997 - Pf6N2E2_1863 0.45 +0.8 2,206,997 - Pf6N2E2_1863 0.45 +0.6 2,206,997 - Pf6N2E2_1863 0.45 +1.2 2,207,251 + Pf6N2E2_1863 0.66 -0.2 2,207,369 - Pf6N2E2_1863 0.75 +0.8 2,207,379 - Pf6N2E2_1863 0.76 +0.8 2,207,379 - Pf6N2E2_1863 0.76 +0.6 2,207,811 - -0.6 2,207,850 - -1.0 2,207,850 - -1.9 2,207,988 + -1.4 2,207,989 - -0.1 2,207,989 - +0.8 2,207,989 - +0.0 2,208,043 + +2.8 2,208,116 + -0.7 2,208,176 + Pf6N2E2_1864 0.12 +1.0 2,208,190 + Pf6N2E2_1864 0.14 -0.2 2,208,191 - Pf6N2E2_1864 0.14 +1.2 2,208,191 - Pf6N2E2_1864 0.14 -0.2 2,208,269 - Pf6N2E2_1864 0.26 +0.9 2,208,315 + Pf6N2E2_1864 0.33 +1.0 2,208,564 + Pf6N2E2_1864 0.71 -0.2 2,208,564 + Pf6N2E2_1864 0.71 +2.3 2,208,565 - Pf6N2E2_1864 0.71 -0.4 2,208,569 - Pf6N2E2_1864 0.72 +0.0 2,208,569 - Pf6N2E2_1864 0.72 +0.8 2,208,569 - Pf6N2E2_1864 0.72 -0.4 2,208,569 - Pf6N2E2_1864 0.72 -2.4 2,208,579 + Pf6N2E2_1864 0.73 +0.5
Or see this region's nucleotide sequence