Experiment: 4-Hydroxybenzoic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2832 and Pf6N2E2_2833 are separated by 329 nucleotides Pf6N2E2_2833 and Pf6N2E2_2834 are separated by 1 nucleotides Pf6N2E2_2834 and Pf6N2E2_2835 are separated by 37 nucleotides
Pf6N2E2_2832 - dicarboxylic acid transporter PcaT, at 3,321,298 to 3,322,596
_2832
Pf6N2E2_2833 - Protocatechuate 3,4-dioxygenase alpha chain (EC 1.13.11.3), at 3,322,926 to 3,323,492
_2833
Pf6N2E2_2834 - Protocatechuate 3,4-dioxygenase beta chain (EC 1.13.11.3), at 3,323,494 to 3,324,198
_2834
Pf6N2E2_2835 - Beta-ketoadipyl CoA thiolase (EC 2.3.1.-), at 3,324,236 to 3,325,438
_2835
Position (kb)
3322
3323
3324 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3322.022 kb on + strand, within Pf6N2E2_2832 at 3322.023 kb on - strand, within Pf6N2E2_2832 at 3322.023 kb on - strand, within Pf6N2E2_2832 at 3322.109 kb on + strand, within Pf6N2E2_2832 at 3322.109 kb on + strand, within Pf6N2E2_2832 at 3322.163 kb on + strand, within Pf6N2E2_2832 at 3322.163 kb on + strand, within Pf6N2E2_2832 at 3322.163 kb on + strand, within Pf6N2E2_2832 at 3322.163 kb on + strand, within Pf6N2E2_2832 at 3322.163 kb on + strand, within Pf6N2E2_2832 at 3322.163 kb on + strand, within Pf6N2E2_2832 at 3322.163 kb on + strand, within Pf6N2E2_2832 at 3322.163 kb on + strand, within Pf6N2E2_2832 at 3322.164 kb on - strand, within Pf6N2E2_2832 at 3322.164 kb on - strand, within Pf6N2E2_2832 at 3322.164 kb on - strand, within Pf6N2E2_2832 at 3322.164 kb on - strand, within Pf6N2E2_2832 at 3322.164 kb on - strand, within Pf6N2E2_2832 at 3322.164 kb on - strand, within Pf6N2E2_2832 at 3322.164 kb on - strand, within Pf6N2E2_2832 at 3322.164 kb on - strand, within Pf6N2E2_2832 at 3322.164 kb on - strand, within Pf6N2E2_2832 at 3322.259 kb on + strand, within Pf6N2E2_2832 at 3322.259 kb on + strand, within Pf6N2E2_2832 at 3322.260 kb on - strand, within Pf6N2E2_2832 at 3322.260 kb on - strand, within Pf6N2E2_2832 at 3322.329 kb on + strand, within Pf6N2E2_2832 at 3322.330 kb on - strand, within Pf6N2E2_2832 at 3322.330 kb on - strand, within Pf6N2E2_2832 at 3322.484 kb on + strand at 3322.484 kb on + strand at 3322.485 kb on - strand at 3322.485 kb on - strand at 3322.485 kb on - strand at 3322.490 kb on + strand at 3322.490 kb on + strand at 3322.490 kb on + strand at 3322.490 kb on + strand at 3322.490 kb on + strand at 3322.490 kb on + strand at 3322.490 kb on + strand at 3322.490 kb on + strand at 3322.490 kb on + strand at 3322.490 kb on + strand at 3322.490 kb on + strand at 3322.490 kb on + strand at 3322.490 kb on + strand at 3322.490 kb on + strand at 3322.491 kb on - strand at 3322.491 kb on - strand at 3322.491 kb on - strand at 3322.491 kb on - strand at 3322.491 kb on - strand at 3322.491 kb on - strand at 3322.491 kb on - strand at 3322.491 kb on - strand at 3322.491 kb on - strand at 3322.491 kb on - strand at 3322.545 kb on + strand at 3322.545 kb on + strand at 3322.546 kb on - strand at 3322.546 kb on - strand at 3322.546 kb on - strand at 3322.572 kb on - strand at 3322.572 kb on - strand at 3322.572 kb on - strand at 3322.602 kb on + strand at 3322.640 kb on - strand at 3322.640 kb on - strand at 3322.640 kb on - strand at 3322.686 kb on + strand at 3322.730 kb on + strand at 3322.730 kb on + strand at 3322.730 kb on + strand at 3322.730 kb on + strand at 3322.731 kb on - strand at 3322.731 kb on - strand at 3322.731 kb on - strand at 3322.747 kb on - strand at 3322.747 kb on - strand at 3322.747 kb on - strand at 3322.747 kb on - strand at 3322.747 kb on - strand at 3322.754 kb on + strand at 3322.754 kb on + strand at 3322.754 kb on + strand at 3322.755 kb on - strand at 3322.853 kb on - strand at 3322.985 kb on - strand, within Pf6N2E2_2833 at 3323.086 kb on + strand, within Pf6N2E2_2833 at 3323.199 kb on - strand, within Pf6N2E2_2833 at 3323.199 kb on - strand, within Pf6N2E2_2833 at 3323.293 kb on + strand, within Pf6N2E2_2833 at 3323.293 kb on + strand, within Pf6N2E2_2833 at 3323.293 kb on + strand, within Pf6N2E2_2833 at 3323.294 kb on - strand, within Pf6N2E2_2833 at 3323.294 kb on - strand, within Pf6N2E2_2833 at 3323.294 kb on - strand, within Pf6N2E2_2833 at 3323.294 kb on - strand, within Pf6N2E2_2833 at 3323.449 kb on + strand at 3323.449 kb on + strand at 3323.450 kb on - strand at 3323.450 kb on - strand at 3323.453 kb on - strand at 3323.453 kb on - strand at 3323.495 kb on + strand at 3323.495 kb on + strand at 3323.495 kb on + strand at 3323.495 kb on + strand at 3323.495 kb on + strand at 3323.496 kb on - strand at 3323.496 kb on - strand at 3323.496 kb on - strand at 3323.496 kb on - strand at 3323.496 kb on - strand at 3323.496 kb on - strand at 3323.496 kb on - strand at 3323.558 kb on + strand at 3323.672 kb on + strand, within Pf6N2E2_2834 at 3323.672 kb on + strand, within Pf6N2E2_2834 at 3323.672 kb on + strand, within Pf6N2E2_2834 at 3323.673 kb on - strand, within Pf6N2E2_2834 at 3323.673 kb on - strand, within Pf6N2E2_2834 at 3323.673 kb on - strand, within Pf6N2E2_2834 at 3323.673 kb on - strand, within Pf6N2E2_2834 at 3323.673 kb on - strand, within Pf6N2E2_2834 at 3323.885 kb on + strand, within Pf6N2E2_2834 at 3323.885 kb on + strand, within Pf6N2E2_2834 at 3323.886 kb on - strand, within Pf6N2E2_2834 at 3324.146 kb on + strand at 3324.146 kb on + strand at 3324.218 kb on + strand at 3324.218 kb on + strand at 3324.219 kb on - strand at 3324.219 kb on - strand at 3324.221 kb on + strand at 3324.221 kb on + strand at 3324.221 kb on + strand at 3324.222 kb on - strand at 3324.224 kb on + strand at 3324.224 kb on + strand at 3324.225 kb on - strand at 3324.225 kb on - strand at 3324.225 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Hydroxybenzoic Acid (C) remove 3,322,022 + Pf6N2E2_2832 0.56 -1.8 3,322,023 - Pf6N2E2_2832 0.56 -0.8 3,322,023 - Pf6N2E2_2832 0.56 -0.6 3,322,109 + Pf6N2E2_2832 0.62 -3.1 3,322,109 + Pf6N2E2_2832 0.62 -3.1 3,322,163 + Pf6N2E2_2832 0.67 -4.0 3,322,163 + Pf6N2E2_2832 0.67 -4.1 3,322,163 + Pf6N2E2_2832 0.67 -3.5 3,322,163 + Pf6N2E2_2832 0.67 -0.3 3,322,163 + Pf6N2E2_2832 0.67 -3.8 3,322,163 + Pf6N2E2_2832 0.67 -1.1 3,322,163 + Pf6N2E2_2832 0.67 -3.8 3,322,163 + Pf6N2E2_2832 0.67 -4.0 3,322,164 - Pf6N2E2_2832 0.67 +0.2 3,322,164 - Pf6N2E2_2832 0.67 -3.8 3,322,164 - Pf6N2E2_2832 0.67 -3.0 3,322,164 - Pf6N2E2_2832 0.67 -1.7 3,322,164 - Pf6N2E2_2832 0.67 -2.4 3,322,164 - Pf6N2E2_2832 0.67 -2.0 3,322,164 - Pf6N2E2_2832 0.67 -1.3 3,322,164 - Pf6N2E2_2832 0.67 -0.1 3,322,164 - Pf6N2E2_2832 0.67 -3.9 3,322,259 + Pf6N2E2_2832 0.74 -4.0 3,322,259 + Pf6N2E2_2832 0.74 -3.4 3,322,260 - Pf6N2E2_2832 0.74 -1.7 3,322,260 - Pf6N2E2_2832 0.74 -3.7 3,322,329 + Pf6N2E2_2832 0.79 -4.2 3,322,330 - Pf6N2E2_2832 0.79 -2.1 3,322,330 - Pf6N2E2_2832 0.79 -1.7 3,322,484 + -1.8 3,322,484 + -3.0 3,322,485 - -2.3 3,322,485 - -1.2 3,322,485 - -3.8 3,322,490 + -1.9 3,322,490 + -4.6 3,322,490 + -0.9 3,322,490 + -3.1 3,322,490 + -3.7 3,322,490 + -3.0 3,322,490 + -3.1 3,322,490 + -4.4 3,322,490 + -2.2 3,322,490 + -4.0 3,322,490 + -2.7 3,322,490 + -1.9 3,322,490 + -4.1 3,322,490 + -2.8 3,322,491 - -0.8 3,322,491 - -2.9 3,322,491 - -2.8 3,322,491 - -3.6 3,322,491 - -1.9 3,322,491 - -3.2 3,322,491 - -2.2 3,322,491 - -3.1 3,322,491 - -3.8 3,322,491 - -1.5 3,322,545 + -2.8 3,322,545 + -2.1 3,322,546 - -2.9 3,322,546 - -0.3 3,322,546 - -1.6 3,322,572 - -1.8 3,322,572 - -1.6 3,322,572 - -2.9 3,322,602 + -3.8 3,322,640 - -1.4 3,322,640 - -1.0 3,322,640 - -0.8 3,322,686 + -3.7 3,322,730 + -3.1 3,322,730 + -2.5 3,322,730 + -2.6 3,322,730 + -3.5 3,322,731 - +0.0 3,322,731 - +1.7 3,322,731 - -0.5 3,322,747 - +0.3 3,322,747 - -0.7 3,322,747 - +0.7 3,322,747 - +0.3 3,322,747 - +2.3 3,322,754 + -3.1 3,322,754 + -3.0 3,322,754 + -3.1 3,322,755 - +0.2 3,322,853 - +0.2 3,322,985 - Pf6N2E2_2833 0.10 -4.3 3,323,086 + Pf6N2E2_2833 0.28 -3.6 3,323,199 - Pf6N2E2_2833 0.48 -1.6 3,323,199 - Pf6N2E2_2833 0.48 -3.8 3,323,293 + Pf6N2E2_2833 0.65 -3.2 3,323,293 + Pf6N2E2_2833 0.65 -2.1 3,323,293 + Pf6N2E2_2833 0.65 -4.0 3,323,294 - Pf6N2E2_2833 0.65 -1.7 3,323,294 - Pf6N2E2_2833 0.65 -1.8 3,323,294 - Pf6N2E2_2833 0.65 -3.3 3,323,294 - Pf6N2E2_2833 0.65 -1.9 3,323,449 + -3.1 3,323,449 + -3.6 3,323,450 - -3.2 3,323,450 - -4.1 3,323,453 - -4.2 3,323,453 - -2.7 3,323,495 + -4.2 3,323,495 + -0.3 3,323,495 + -3.9 3,323,495 + -2.5 3,323,495 + -3.3 3,323,496 - -2.1 3,323,496 - -3.7 3,323,496 - -1.1 3,323,496 - -0.3 3,323,496 - -2.1 3,323,496 - -1.9 3,323,496 - -0.8 3,323,558 + -1.7 3,323,672 + Pf6N2E2_2834 0.25 -0.7 3,323,672 + Pf6N2E2_2834 0.25 -0.6 3,323,672 + Pf6N2E2_2834 0.25 -2.3 3,323,673 - Pf6N2E2_2834 0.25 -3.4 3,323,673 - Pf6N2E2_2834 0.25 -3.3 3,323,673 - Pf6N2E2_2834 0.25 -4.2 3,323,673 - Pf6N2E2_2834 0.25 -3.9 3,323,673 - Pf6N2E2_2834 0.25 -3.5 3,323,885 + Pf6N2E2_2834 0.55 -1.3 3,323,885 + Pf6N2E2_2834 0.55 -2.3 3,323,886 - Pf6N2E2_2834 0.56 -3.6 3,324,146 + -3.2 3,324,146 + -1.6 3,324,218 + -1.3 3,324,218 + -2.7 3,324,219 - -0.3 3,324,219 - +0.4 3,324,221 + -2.3 3,324,221 + -2.1 3,324,221 + -3.7 3,324,222 - +0.0 3,324,224 + +0.3 3,324,224 + -2.1 3,324,225 - -3.7 3,324,225 - -1.6 3,324,225 - -1.5
Or see this region's nucleotide sequence