Experiment: Mc formate, casamino free, with 5mM L-Glutamate and 5mM L-Proline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07570 and MMP_RS07575 are separated by 15 nucleotides MMP_RS07575 and MMP_RS07580 are separated by 55 nucleotides MMP_RS07580 and MMP_RS07585 are separated by 39 nucleotides
MMP_RS07570: MMP_RS07570 - nucleotidyltransferase family protein, at 1,431,231 to 1,432,280
_RS07570
MMP_RS07575: MMP_RS07575 - RNA ligase partner protein, at 1,432,296 to 1,432,967
_RS07575
MMP_RS07580: MMP_RS07580 - DUF356 domain-containing protein, at 1,433,023 to 1,433,403
_RS07580
MMP_RS07585: MMP_RS07585 - isoleucine--tRNA ligase, at 1,433,443 to 1,436,547
_RS07585
Position (kb)
1432
1433 Strain fitness (log2 ratio)
-2
-1
0
1 at 1431.503 kb on - strand, within MMP_RS07570 at 1431.683 kb on + strand, within MMP_RS07570 at 1431.729 kb on + strand, within MMP_RS07570 at 1431.729 kb on + strand, within MMP_RS07570 at 1431.731 kb on - strand, within MMP_RS07570 at 1431.731 kb on - strand, within MMP_RS07570 at 1431.786 kb on - strand, within MMP_RS07570 at 1431.787 kb on + strand, within MMP_RS07570 at 1431.787 kb on + strand, within MMP_RS07570 at 1431.807 kb on - strand, within MMP_RS07570 at 1431.807 kb on - strand, within MMP_RS07570 at 1431.932 kb on - strand, within MMP_RS07570 at 1431.942 kb on + strand, within MMP_RS07570 at 1432.034 kb on - strand, within MMP_RS07570 at 1432.035 kb on + strand, within MMP_RS07570 at 1432.062 kb on - strand, within MMP_RS07570 at 1432.077 kb on + strand, within MMP_RS07570 at 1432.108 kb on + strand, within MMP_RS07570 at 1432.134 kb on - strand, within MMP_RS07570 at 1432.212 kb on + strand at 1432.222 kb on + strand at 1432.322 kb on - strand at 1432.323 kb on + strand at 1432.585 kb on - strand, within MMP_RS07575 at 1432.585 kb on - strand, within MMP_RS07575 at 1432.599 kb on + strand, within MMP_RS07575 at 1432.678 kb on + strand, within MMP_RS07575 at 1432.984 kb on + strand at 1433.098 kb on + strand, within MMP_RS07580 at 1433.103 kb on + strand, within MMP_RS07580 at 1433.109 kb on + strand, within MMP_RS07580 at 1433.309 kb on + strand, within MMP_RS07580 at 1433.354 kb on + strand, within MMP_RS07580 at 1433.412 kb on + strand at 1433.799 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 5mM L-Glutamate and 5mM L-Proline remove 1,431,503 - MMP_RS07570 0.26 +0.3 1,431,683 + MMP_RS07570 0.43 -1.2 1,431,729 + MMP_RS07570 0.47 +1.2 1,431,729 + MMP_RS07570 0.47 -1.4 1,431,731 - MMP_RS07570 0.48 +0.2 1,431,731 - MMP_RS07570 0.48 +0.5 1,431,786 - MMP_RS07570 0.53 -2.1 1,431,787 + MMP_RS07570 0.53 -1.6 1,431,787 + MMP_RS07570 0.53 +0.9 1,431,807 - MMP_RS07570 0.55 +0.3 1,431,807 - MMP_RS07570 0.55 +1.0 1,431,932 - MMP_RS07570 0.67 +1.1 1,431,942 + MMP_RS07570 0.68 -1.1 1,432,034 - MMP_RS07570 0.76 +0.5 1,432,035 + MMP_RS07570 0.77 +0.4 1,432,062 - MMP_RS07570 0.79 +0.7 1,432,077 + MMP_RS07570 0.81 +0.7 1,432,108 + MMP_RS07570 0.84 +0.8 1,432,134 - MMP_RS07570 0.86 -0.0 1,432,212 + +0.4 1,432,222 + +0.8 1,432,322 - -1.9 1,432,323 + -1.8 1,432,585 - MMP_RS07575 0.43 -2.3 1,432,585 - MMP_RS07575 0.43 -2.2 1,432,599 + MMP_RS07575 0.45 -1.5 1,432,678 + MMP_RS07575 0.57 -1.0 1,432,984 + -1.1 1,433,098 + MMP_RS07580 0.20 -1.1 1,433,103 + MMP_RS07580 0.21 +0.7 1,433,109 + MMP_RS07580 0.23 -1.0 1,433,309 + MMP_RS07580 0.75 -0.8 1,433,354 + MMP_RS07580 0.87 -0.7 1,433,412 + +0.8 1,433,799 - -2.1
Or see this region's nucleotide sequence