Experiment: Mc formate, casamino free, with 5mM L-Glutamate and 5mM L-Proline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06965 and MMP_RS06970 are separated by 6 nucleotides MMP_RS06970 and MMP_RS06975 are separated by 170 nucleotides MMP_RS06975 and MMP_RS06980 are separated by 90 nucleotides
MMP_RS06965: MMP_RS06965 - TIGR04014 family B12-binding domain/radical SAM domain-containing protein, at 1,329,745 to 1,331,058
_RS06965
MMP_RS06970: MMP_RS06970 - ribosomal protein S18-alanine N-acetyltransferase, at 1,331,065 to 1,331,550
_RS06970
MMP_RS06975: MMP_RS06975 - sulfide-dependent adenosine diphosphate thiazole synthase, at 1,331,721 to 1,332,509
_RS06975
MMP_RS06980: MMP_RS06980 - tRNA 2-thiocytidine biosynthesis TtcA family protein, at 1,332,600 to 1,333,574
_RS06980
Position (kb)
1331
1332
1333 Strain fitness (log2 ratio)
-2
-1
0
1 at 1331.008 kb on - strand at 1331.008 kb on - strand at 1331.043 kb on + strand at 1331.063 kb on + strand at 1331.129 kb on + strand, within MMP_RS06970 at 1331.151 kb on + strand, within MMP_RS06970 at 1331.154 kb on + strand, within MMP_RS06970 at 1331.154 kb on + strand, within MMP_RS06970 at 1331.157 kb on - strand, within MMP_RS06970 at 1331.157 kb on - strand, within MMP_RS06970 at 1331.159 kb on - strand, within MMP_RS06970 at 1331.286 kb on - strand, within MMP_RS06970 at 1331.323 kb on - strand, within MMP_RS06970 at 1331.347 kb on + strand, within MMP_RS06970 at 1331.442 kb on + strand, within MMP_RS06970 at 1331.442 kb on + strand, within MMP_RS06970 at 1331.443 kb on - strand, within MMP_RS06970 at 1331.444 kb on + strand, within MMP_RS06970 at 1331.542 kb on - strand at 1331.551 kb on - strand at 1331.551 kb on - strand at 1331.553 kb on - strand at 1331.553 kb on - strand at 1331.554 kb on + strand at 1331.554 kb on + strand at 1331.554 kb on + strand at 1331.593 kb on + strand at 1331.598 kb on + strand at 1331.600 kb on + strand at 1331.666 kb on - strand at 1331.708 kb on + strand at 1331.776 kb on - strand at 1331.876 kb on - strand, within MMP_RS06975 at 1332.005 kb on + strand, within MMP_RS06975 at 1332.005 kb on + strand, within MMP_RS06975 at 1332.545 kb on + strand at 1332.613 kb on - strand at 1332.613 kb on - strand at 1332.725 kb on - strand, within MMP_RS06980 at 1332.726 kb on + strand, within MMP_RS06980 at 1332.761 kb on + strand, within MMP_RS06980 at 1332.873 kb on - strand, within MMP_RS06980 at 1332.874 kb on + strand, within MMP_RS06980 at 1332.916 kb on + strand, within MMP_RS06980 at 1332.919 kb on + strand, within MMP_RS06980 at 1332.920 kb on - strand, within MMP_RS06980 at 1332.934 kb on + strand, within MMP_RS06980 at 1332.947 kb on - strand, within MMP_RS06980 at 1332.947 kb on - strand, within MMP_RS06980 at 1333.237 kb on + strand, within MMP_RS06980 at 1333.362 kb on - strand, within MMP_RS06980 at 1333.506 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 5mM L-Glutamate and 5mM L-Proline remove 1,331,008 - -0.2 1,331,008 - -1.4 1,331,043 + +1.2 1,331,063 + +0.4 1,331,129 + MMP_RS06970 0.13 +0.2 1,331,151 + MMP_RS06970 0.18 -1.3 1,331,154 + MMP_RS06970 0.18 -1.3 1,331,154 + MMP_RS06970 0.18 -1.0 1,331,157 - MMP_RS06970 0.19 +0.6 1,331,157 - MMP_RS06970 0.19 +0.3 1,331,159 - MMP_RS06970 0.19 -0.2 1,331,286 - MMP_RS06970 0.45 -0.8 1,331,323 - MMP_RS06970 0.53 +0.6 1,331,347 + MMP_RS06970 0.58 +0.8 1,331,442 + MMP_RS06970 0.78 -1.3 1,331,442 + MMP_RS06970 0.78 -2.7 1,331,443 - MMP_RS06970 0.78 -0.2 1,331,444 + MMP_RS06970 0.78 -0.7 1,331,542 - -0.0 1,331,551 - +0.1 1,331,551 - +0.5 1,331,553 - -0.6 1,331,553 - +0.6 1,331,554 + -0.6 1,331,554 + -0.1 1,331,554 + -0.9 1,331,593 + -1.4 1,331,598 + -0.6 1,331,600 + +0.1 1,331,666 - +0.7 1,331,708 + -0.6 1,331,776 - -1.3 1,331,876 - MMP_RS06975 0.20 -1.2 1,332,005 + MMP_RS06975 0.36 -2.1 1,332,005 + MMP_RS06975 0.36 -2.7 1,332,545 + -0.5 1,332,613 - -1.1 1,332,613 - -1.5 1,332,725 - MMP_RS06980 0.13 +0.9 1,332,726 + MMP_RS06980 0.13 +0.7 1,332,761 + MMP_RS06980 0.17 -2.2 1,332,873 - MMP_RS06980 0.28 -0.8 1,332,874 + MMP_RS06980 0.28 +0.3 1,332,916 + MMP_RS06980 0.32 +0.5 1,332,919 + MMP_RS06980 0.33 +0.2 1,332,920 - MMP_RS06980 0.33 -0.4 1,332,934 + MMP_RS06980 0.34 +0.1 1,332,947 - MMP_RS06980 0.36 +0.1 1,332,947 - MMP_RS06980 0.36 -0.8 1,333,237 + MMP_RS06980 0.65 +0.0 1,333,362 - MMP_RS06980 0.78 +0.1 1,333,506 + -0.1
Or see this region's nucleotide sequence