Strain Fitness in Methanococcus maripaludis S2 around MMP_RS06400

Experiment: Mc formate, casamino free, with 5mM L-Glutamate and 5mM L-Proline

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS06390 and MMP_RS06395 are separated by 52 nucleotidesMMP_RS06395 and MMP_RS06400 are separated by 203 nucleotidesMMP_RS06400 and MMP_RS06405 are separated by 23 nucleotides MMP_RS06390: MMP_RS06390 - quinolinate synthase NadA, at 1,227,185 to 1,228,102 _RS06390 MMP_RS06395: MMP_RS06395 - ThiF family adenylyltransferase, at 1,228,155 to 1,228,769 _RS06395 MMP_RS06400: MMP_RS06400 - 4Fe-4S binding protein, at 1,228,973 to 1,229,419 _RS06400 MMP_RS06405: MMP_RS06405 - 4Fe-4S binding protein, at 1,229,443 to 1,230,501 _RS06405 Position (kb) 1228 1229 1230Strain fitness (log2 ratio) -3 -2 -1 0 1at 1228.036 kb on + strandat 1228.094 kb on - strandat 1228.094 kb on - strandat 1228.416 kb on - strand, within MMP_RS06395at 1228.509 kb on + strand, within MMP_RS06395at 1228.568 kb on + strand, within MMP_RS06395at 1228.603 kb on + strand, within MMP_RS06395at 1228.798 kb on + strandat 1228.837 kb on + strandat 1228.928 kb on - strandat 1228.929 kb on + strandat 1228.929 kb on + strandat 1228.929 kb on + strandat 1228.929 kb on + strandat 1228.951 kb on + strandat 1228.985 kb on + strandat 1228.985 kb on + strandat 1228.985 kb on + strandat 1228.986 kb on - strandat 1228.986 kb on - strandat 1228.987 kb on + strandat 1228.987 kb on + strandat 1228.990 kb on + strandat 1229.234 kb on - strand, within MMP_RS06400at 1229.242 kb on + strand, within MMP_RS06400at 1229.251 kb on - strand, within MMP_RS06400at 1229.439 kb on - strandat 1229.440 kb on + strandat 1230.027 kb on + strandat 1230.083 kb on + strandat 1230.357 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 5mM L-Glutamate and 5mM L-Proline
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1,228,036 + -0.8
1,228,094 - -1.1
1,228,094 - -2.2
1,228,416 - MMP_RS06395 0.42 +1.2
1,228,509 + MMP_RS06395 0.58 +0.9
1,228,568 + MMP_RS06395 0.67 +1.0
1,228,603 + MMP_RS06395 0.73 +0.9
1,228,798 + +0.5
1,228,837 + -0.5
1,228,928 - -1.1
1,228,929 + -1.7
1,228,929 + -1.6
1,228,929 + -0.9
1,228,929 + -1.9
1,228,951 + -3.2
1,228,985 + -1.8
1,228,985 + -1.3
1,228,985 + -2.6
1,228,986 - -1.5
1,228,986 - -2.7
1,228,987 + -0.9
1,228,987 + -2.3
1,228,990 + -3.2
1,229,234 - MMP_RS06400 0.58 -1.1
1,229,242 + MMP_RS06400 0.60 -2.7
1,229,251 - MMP_RS06400 0.62 -1.0
1,229,439 - +0.7
1,229,440 + -1.6
1,230,027 + -0.9
1,230,083 + -1.3
1,230,357 + -0.1

Or see this region's nucleotide sequence