Experiment: Mc formate, casamino free, with 5mM L-Glutamate and 5mM L-Proline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05915 and MMP_RS05920 are separated by 174 nucleotides MMP_RS05920 and MMP_RS05925 are separated by 76 nucleotides MMP_RS05925 and MMP_RS05930 are separated by 131 nucleotides MMP_RS05930 and MMP_RS05935 are separated by 76 nucleotides
MMP_RS05915: MMP_RS05915 - amidophosphoribosyltransferase, at 1,135,617 to 1,136,996
_RS05915
MMP_RS05920: MMP_RS05920 - 50S ribosomal protein L37e, at 1,137,171 to 1,137,365
_RS05920
MMP_RS05925: MMP_RS05925 - small nuclear ribonucleoprotein, at 1,137,442 to 1,137,660
_RS05925
MMP_RS05930: MMP_RS05930 - 3-isopropylmalate dehydratase large subunit, at 1,137,792 to 1,139,066
_RS05930
MMP_RS05935: MMP_RS05935 - uroporphyrinogen decarboxylase family protein, at 1,139,143 to 1,140,201
_RS05935
Position (kb)
1137
1138
1139
1140 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1136.865 kb on - strand at 1137.017 kb on + strand at 1137.024 kb on - strand at 1137.026 kb on - strand at 1137.027 kb on + strand at 1137.029 kb on + strand at 1137.029 kb on + strand at 1137.029 kb on + strand at 1137.082 kb on - strand at 1137.084 kb on - strand at 1137.084 kb on - strand at 1137.084 kb on - strand at 1137.085 kb on + strand at 1137.085 kb on + strand at 1137.085 kb on + strand at 1137.085 kb on + strand at 1137.106 kb on - strand at 1137.117 kb on - strand at 1137.145 kb on + strand at 1137.149 kb on + strand at 1137.149 kb on + strand at 1137.338 kb on - strand, within MMP_RS05920 at 1137.387 kb on - strand at 1137.392 kb on - strand at 1137.399 kb on - strand at 1137.445 kb on - strand at 1137.456 kb on + strand at 1137.460 kb on - strand at 1137.460 kb on - strand at 1137.476 kb on + strand, within MMP_RS05925 at 1137.554 kb on - strand, within MMP_RS05925 at 1137.554 kb on - strand, within MMP_RS05925 at 1137.562 kb on - strand, within MMP_RS05925 at 1137.593 kb on + strand, within MMP_RS05925 at 1137.624 kb on - strand, within MMP_RS05925 at 1137.660 kb on - strand at 1137.678 kb on - strand at 1137.732 kb on + strand at 1137.734 kb on + strand at 1137.748 kb on + strand at 1137.748 kb on + strand at 1137.776 kb on + strand at 1137.777 kb on - strand at 1137.903 kb on + strand at 1137.942 kb on + strand, within MMP_RS05930 at 1138.030 kb on - strand, within MMP_RS05930 at 1138.061 kb on - strand, within MMP_RS05930 at 1138.400 kb on - strand, within MMP_RS05930 at 1138.498 kb on + strand, within MMP_RS05930 at 1139.111 kb on - strand at 1139.321 kb on + strand, within MMP_RS05935 at 1139.335 kb on - strand, within MMP_RS05935 at 1139.336 kb on + strand, within MMP_RS05935 at 1139.354 kb on + strand, within MMP_RS05935 at 1139.496 kb on - strand, within MMP_RS05935 at 1139.499 kb on - strand, within MMP_RS05935 at 1139.500 kb on + strand, within MMP_RS05935 at 1139.540 kb on - strand, within MMP_RS05935 at 1139.575 kb on - strand, within MMP_RS05935 at 1139.690 kb on + strand, within MMP_RS05935 at 1139.703 kb on - strand, within MMP_RS05935 at 1139.705 kb on - strand, within MMP_RS05935 at 1139.705 kb on - strand, within MMP_RS05935 at 1139.706 kb on + strand, within MMP_RS05935 at 1139.706 kb on + strand, within MMP_RS05935 at 1139.706 kb on + strand, within MMP_RS05935 at 1139.709 kb on - strand, within MMP_RS05935 at 1139.828 kb on - strand, within MMP_RS05935 at 1139.832 kb on + strand, within MMP_RS05935 at 1139.834 kb on + strand, within MMP_RS05935 at 1139.937 kb on + strand, within MMP_RS05935 at 1139.970 kb on + strand, within MMP_RS05935 at 1139.970 kb on + strand, within MMP_RS05935 at 1139.983 kb on + strand, within MMP_RS05935 at 1139.986 kb on - strand, within MMP_RS05935
Per-strain Table
Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 5mM L-Glutamate and 5mM L-Proline remove 1,136,865 - -2.3 1,137,017 + -0.5 1,137,024 - -0.3 1,137,026 - -2.6 1,137,027 + -2.7 1,137,029 + -0.8 1,137,029 + -0.7 1,137,029 + -0.8 1,137,082 - -2.6 1,137,084 - +0.9 1,137,084 - +0.4 1,137,084 - +0.4 1,137,085 + +2.2 1,137,085 + +0.2 1,137,085 + +0.2 1,137,085 + +1.1 1,137,106 - -1.0 1,137,117 - -0.4 1,137,145 + +0.3 1,137,149 + -1.7 1,137,149 + -0.4 1,137,338 - MMP_RS05920 0.86 -0.3 1,137,387 - -0.6 1,137,392 - -1.3 1,137,399 - -0.0 1,137,445 - -0.0 1,137,456 + +0.2 1,137,460 - -0.2 1,137,460 - -0.9 1,137,476 + MMP_RS05925 0.16 -0.4 1,137,554 - MMP_RS05925 0.51 -0.6 1,137,554 - MMP_RS05925 0.51 -1.9 1,137,562 - MMP_RS05925 0.55 -0.8 1,137,593 + MMP_RS05925 0.69 -1.5 1,137,624 - MMP_RS05925 0.83 -0.2 1,137,660 - -0.4 1,137,678 - +0.1 1,137,732 + -0.5 1,137,734 + -3.7 1,137,748 + -2.1 1,137,748 + -1.5 1,137,776 + +0.2 1,137,777 - -2.0 1,137,903 + -1.3 1,137,942 + MMP_RS05930 0.12 -0.4 1,138,030 - MMP_RS05930 0.19 -0.9 1,138,061 - MMP_RS05930 0.21 -3.3 1,138,400 - MMP_RS05930 0.48 -1.1 1,138,498 + MMP_RS05930 0.55 -2.3 1,139,111 - +0.6 1,139,321 + MMP_RS05935 0.17 +0.4 1,139,335 - MMP_RS05935 0.18 -0.1 1,139,336 + MMP_RS05935 0.18 +0.2 1,139,354 + MMP_RS05935 0.20 +0.3 1,139,496 - MMP_RS05935 0.33 -0.7 1,139,499 - MMP_RS05935 0.34 -1.6 1,139,500 + MMP_RS05935 0.34 +0.7 1,139,540 - MMP_RS05935 0.37 -0.3 1,139,575 - MMP_RS05935 0.41 -0.5 1,139,690 + MMP_RS05935 0.52 -0.5 1,139,703 - MMP_RS05935 0.53 -0.6 1,139,705 - MMP_RS05935 0.53 +0.4 1,139,705 - MMP_RS05935 0.53 +0.8 1,139,706 + MMP_RS05935 0.53 +0.4 1,139,706 + MMP_RS05935 0.53 +0.5 1,139,706 + MMP_RS05935 0.53 +0.6 1,139,709 - MMP_RS05935 0.53 +0.8 1,139,828 - MMP_RS05935 0.65 +0.2 1,139,832 + MMP_RS05935 0.65 -0.3 1,139,834 + MMP_RS05935 0.65 +0.8 1,139,937 + MMP_RS05935 0.75 -0.4 1,139,970 + MMP_RS05935 0.78 -0.2 1,139,970 + MMP_RS05935 0.78 -0.7 1,139,983 + MMP_RS05935 0.79 +0.5 1,139,986 - MMP_RS05935 0.80 -0.8
Or see this region's nucleotide sequence