Experiment: Mc formate, casamino free, with 5mM L-Glutamate and 5mM L-Proline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05050 and MMP_RS05055 are separated by 219 nucleotides MMP_RS05055 and MMP_RS05060 are separated by 0 nucleotides MMP_RS05060 and MMP_RS05065 are separated by 4 nucleotides MMP_RS05065 and MMP_RS05070 are separated by 104 nucleotides MMP_RS05070 and MMP_RS05075 are separated by 4 nucleotides
MMP_RS05050: MMP_RS05050 - substrate-binding domain-containing protein, at 961,541 to 962,392
_RS05050
MMP_RS05055: MMP_RS05055 - DUF166 domain-containing protein, at 962,612 to 963,268
_RS05055
MMP_RS05060: MMP_RS05060 - AAA family ATPase, at 963,269 to 964,051
_RS05060
MMP_RS05065: MMP_RS05065 - CooT family nickel-binding protein, at 964,056 to 964,235
_RS05065
MMP_RS05070: MMP_RS05070 - 4Fe-4S dicluster domain-containing protein, at 964,340 to 964,804
_RS05070
MMP_RS05075: MMP_RS05075 - acetyl-CoA decarbonylase/synthase complex subunit gamma, at 964,809 to 966,182
_RS05075
Position (kb)
963
964
965 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 962.379 kb on - strand at 962.395 kb on + strand at 962.395 kb on + strand at 962.452 kb on + strand at 962.460 kb on + strand at 962.463 kb on + strand at 962.467 kb on + strand at 962.487 kb on - strand at 962.488 kb on + strand at 962.488 kb on + strand at 962.499 kb on - strand at 962.545 kb on - strand at 962.564 kb on - strand at 962.565 kb on + strand at 962.566 kb on - strand at 962.566 kb on - strand at 962.566 kb on - strand at 962.567 kb on + strand at 962.568 kb on - strand at 962.568 kb on - strand at 962.569 kb on + strand at 962.569 kb on + strand at 962.569 kb on + strand at 962.569 kb on + strand at 962.569 kb on + strand at 962.573 kb on + strand at 962.573 kb on + strand at 962.598 kb on + strand at 962.620 kb on + strand at 962.625 kb on - strand at 962.663 kb on + strand at 962.735 kb on + strand, within MMP_RS05055 at 962.779 kb on - strand, within MMP_RS05055 at 962.788 kb on - strand, within MMP_RS05055 at 962.789 kb on + strand, within MMP_RS05055 at 962.792 kb on - strand, within MMP_RS05055 at 962.792 kb on - strand, within MMP_RS05055 at 962.792 kb on - strand, within MMP_RS05055 at 962.792 kb on - strand, within MMP_RS05055 at 962.793 kb on + strand, within MMP_RS05055 at 962.794 kb on - strand, within MMP_RS05055 at 962.794 kb on - strand, within MMP_RS05055 at 962.794 kb on - strand, within MMP_RS05055 at 962.794 kb on - strand, within MMP_RS05055 at 962.794 kb on - strand, within MMP_RS05055 at 962.794 kb on - strand, within MMP_RS05055 at 962.795 kb on + strand, within MMP_RS05055 at 962.795 kb on + strand, within MMP_RS05055 at 962.799 kb on - strand, within MMP_RS05055 at 962.837 kb on - strand, within MMP_RS05055 at 962.838 kb on + strand, within MMP_RS05055 at 962.852 kb on - strand, within MMP_RS05055 at 963.023 kb on + strand, within MMP_RS05055 at 963.039 kb on - strand, within MMP_RS05055 at 963.040 kb on + strand, within MMP_RS05055 at 963.047 kb on + strand, within MMP_RS05055 at 963.047 kb on + strand, within MMP_RS05055 at 963.049 kb on + strand, within MMP_RS05055 at 963.049 kb on + strand, within MMP_RS05055 at 963.049 kb on + strand, within MMP_RS05055 at 963.137 kb on + strand, within MMP_RS05055 at 963.366 kb on - strand, within MMP_RS05060 at 963.366 kb on - strand, within MMP_RS05060 at 963.367 kb on + strand, within MMP_RS05060 at 963.408 kb on - strand, within MMP_RS05060 at 963.409 kb on + strand, within MMP_RS05060 at 963.448 kb on + strand, within MMP_RS05060 at 963.548 kb on - strand, within MMP_RS05060 at 963.637 kb on + strand, within MMP_RS05060 at 963.745 kb on + strand, within MMP_RS05060 at 963.778 kb on - strand, within MMP_RS05060 at 963.847 kb on + strand, within MMP_RS05060 at 963.916 kb on + strand, within MMP_RS05060 at 963.919 kb on + strand, within MMP_RS05060 at 963.945 kb on - strand, within MMP_RS05060 at 963.946 kb on + strand, within MMP_RS05060 at 964.013 kb on + strand at 964.060 kb on + strand at 964.075 kb on + strand, within MMP_RS05065 at 964.075 kb on + strand, within MMP_RS05065 at 964.075 kb on + strand, within MMP_RS05065 at 964.138 kb on + strand, within MMP_RS05065 at 964.197 kb on - strand, within MMP_RS05065 at 964.235 kb on - strand at 964.267 kb on - strand at 964.288 kb on + strand at 964.301 kb on + strand at 964.304 kb on + strand at 964.354 kb on + strand at 964.402 kb on - strand, within MMP_RS05070 at 964.421 kb on - strand, within MMP_RS05070 at 964.533 kb on + strand, within MMP_RS05070 at 964.533 kb on + strand, within MMP_RS05070 at 964.537 kb on - strand, within MMP_RS05070 at 964.537 kb on - strand, within MMP_RS05070 at 964.581 kb on + strand, within MMP_RS05070 at 964.581 kb on + strand, within MMP_RS05070 at 964.582 kb on - strand, within MMP_RS05070 at 964.601 kb on - strand, within MMP_RS05070 at 964.751 kb on + strand, within MMP_RS05070 at 964.787 kb on - strand at 964.816 kb on + strand at 964.824 kb on - strand at 964.825 kb on + strand at 964.866 kb on + strand at 964.891 kb on + strand at 964.947 kb on - strand, within MMP_RS05075
Per-strain Table
Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 5mM L-Glutamate and 5mM L-Proline remove 962,379 - -2.2 962,395 + +0.2 962,395 + +0.6 962,452 + +0.8 962,460 + +0.6 962,463 + +0.1 962,467 + -0.2 962,487 - +0.4 962,488 + -0.5 962,488 + +0.8 962,499 - -0.9 962,545 - +0.2 962,564 - +0.4 962,565 + -0.5 962,566 - -1.8 962,566 - -4.9 962,566 - -0.6 962,567 + -0.9 962,568 - -0.7 962,568 - -0.3 962,569 + -0.4 962,569 + -0.9 962,569 + -1.2 962,569 + -0.2 962,569 + +0.4 962,573 + +0.1 962,573 + -1.0 962,598 + -1.0 962,620 + -1.9 962,625 - -3.4 962,663 + -1.7 962,735 + MMP_RS05055 0.19 -2.2 962,779 - MMP_RS05055 0.25 -1.4 962,788 - MMP_RS05055 0.27 -1.8 962,789 + MMP_RS05055 0.27 -2.6 962,792 - MMP_RS05055 0.27 -2.7 962,792 - MMP_RS05055 0.27 -1.9 962,792 - MMP_RS05055 0.27 -2.5 962,792 - MMP_RS05055 0.27 -1.5 962,793 + MMP_RS05055 0.28 -2.1 962,794 - MMP_RS05055 0.28 -2.8 962,794 - MMP_RS05055 0.28 -2.0 962,794 - MMP_RS05055 0.28 -1.9 962,794 - MMP_RS05055 0.28 -1.7 962,794 - MMP_RS05055 0.28 -1.6 962,794 - MMP_RS05055 0.28 -1.8 962,795 + MMP_RS05055 0.28 -2.5 962,795 + MMP_RS05055 0.28 -1.8 962,799 - MMP_RS05055 0.28 -1.4 962,837 - MMP_RS05055 0.34 -2.0 962,838 + MMP_RS05055 0.34 -2.6 962,852 - MMP_RS05055 0.37 -1.4 963,023 + MMP_RS05055 0.63 -2.8 963,039 - MMP_RS05055 0.65 -2.0 963,040 + MMP_RS05055 0.65 -2.0 963,047 + MMP_RS05055 0.66 -4.5 963,047 + MMP_RS05055 0.66 -1.2 963,049 + MMP_RS05055 0.67 -1.5 963,049 + MMP_RS05055 0.67 -1.6 963,049 + MMP_RS05055 0.67 -0.8 963,137 + MMP_RS05055 0.80 -1.8 963,366 - MMP_RS05060 0.12 -2.8 963,366 - MMP_RS05060 0.12 -2.1 963,367 + MMP_RS05060 0.13 -1.6 963,408 - MMP_RS05060 0.18 -4.1 963,409 + MMP_RS05060 0.18 -2.8 963,448 + MMP_RS05060 0.23 -2.9 963,548 - MMP_RS05060 0.36 -1.2 963,637 + MMP_RS05060 0.47 -1.5 963,745 + MMP_RS05060 0.61 -1.3 963,778 - MMP_RS05060 0.65 -1.2 963,847 + MMP_RS05060 0.74 -2.0 963,916 + MMP_RS05060 0.83 -1.5 963,919 + MMP_RS05060 0.83 -1.8 963,945 - MMP_RS05060 0.86 -0.9 963,946 + MMP_RS05060 0.86 -1.9 964,013 + -2.2 964,060 + -1.7 964,075 + MMP_RS05065 0.11 -1.3 964,075 + MMP_RS05065 0.11 -2.5 964,075 + MMP_RS05065 0.11 -0.8 964,138 + MMP_RS05065 0.46 -1.4 964,197 - MMP_RS05065 0.78 -1.6 964,235 - -1.4 964,267 - -1.9 964,288 + -1.3 964,301 + -1.2 964,304 + -1.8 964,354 + +0.1 964,402 - MMP_RS05070 0.13 -1.6 964,421 - MMP_RS05070 0.17 -0.8 964,533 + MMP_RS05070 0.42 -1.3 964,533 + MMP_RS05070 0.42 -1.8 964,537 - MMP_RS05070 0.42 -2.3 964,537 - MMP_RS05070 0.42 -2.7 964,581 + MMP_RS05070 0.52 -1.7 964,581 + MMP_RS05070 0.52 -1.3 964,582 - MMP_RS05070 0.52 -1.4 964,601 - MMP_RS05070 0.56 -3.0 964,751 + MMP_RS05070 0.88 -1.8 964,787 - -2.1 964,816 + -1.7 964,824 - -1.6 964,825 + -2.3 964,866 + -2.0 964,891 + -2.0 964,947 - MMP_RS05075 0.10 -1.3
Or see this region's nucleotide sequence