Experiment: Mc formate, casamino free, with 5mM L-Glutamate and 5mM L-Proline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04245 and MMP_RS04250 are separated by 325 nucleotides MMP_RS04250 and MMP_RS04255 are separated by 152 nucleotides MMP_RS04255 and MMP_RS04260 are separated by 21 nucleotides MMP_RS04260 and MMP_RS04265 are separated by 10 nucleotides
MMP_RS04245: MMP_RS04245 - right-handed parallel beta-helix repeat-containing protein, at 808,687 to 810,939
_RS04245
MMP_RS04250: MMP_RS04250 - PC4/YdbC family ssDNA-binding protein, at 811,265 to 811,486
_RS04250
MMP_RS04255: MMP_RS04255 - coenzyme F420 hydrogenase subunit beta, at 811,639 to 812,487
_RS04255
MMP_RS04260: MMP_RS04260 - coenzyme F420 hydrogenase subunit gamma, at 812,509 to 813,237
_RS04260
MMP_RS04265: MMP_RS04265 - coenzyme F420-reducing hydrogenase FrhD protein, at 813,248 to 813,748
_RS04265
Position (kb) 811
812
813 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 810.639 kb on - strand, within MMP_RS04245 at 810.857 kb on - strand at 810.874 kb on - strand at 810.875 kb on + strand at 810.898 kb on + strand at 810.903 kb on - strand at 810.903 kb on - strand at 810.904 kb on + strand at 810.916 kb on + strand at 810.917 kb on - strand at 810.931 kb on - strand at 810.931 kb on - strand at 810.973 kb on - strand at 811.009 kb on - strand at 811.010 kb on + strand at 811.010 kb on + strand at 811.034 kb on - strand at 811.034 kb on - strand at 811.035 kb on + strand at 811.036 kb on - strand at 811.036 kb on - strand at 811.036 kb on - strand at 811.037 kb on + strand at 811.053 kb on + strand at 811.064 kb on - strand at 811.068 kb on - strand at 811.069 kb on + strand at 811.185 kb on - strand at 811.186 kb on + strand at 811.189 kb on - strand at 811.213 kb on + strand at 811.244 kb on + strand at 811.324 kb on - strand, within MMP_RS04250 at 811.368 kb on + strand, within MMP_RS04250 at 811.368 kb on + strand, within MMP_RS04250 at 811.400 kb on - strand, within MMP_RS04250 at 811.401 kb on + strand, within MMP_RS04250 at 811.441 kb on + strand, within MMP_RS04250 at 811.445 kb on - strand, within MMP_RS04250 at 811.453 kb on - strand, within MMP_RS04250 at 811.464 kb on + strand at 811.514 kb on + strand at 811.525 kb on + strand at 811.552 kb on - strand at 811.553 kb on + strand at 811.560 kb on - strand at 811.601 kb on - strand at 811.607 kb on - strand at 811.607 kb on - strand at 811.726 kb on - strand, within MMP_RS04255 at 811.872 kb on - strand, within MMP_RS04255 at 811.925 kb on - strand, within MMP_RS04255 at 811.925 kb on - strand, within MMP_RS04255 at 811.926 kb on + strand, within MMP_RS04255 at 811.935 kb on - strand, within MMP_RS04255 at 812.048 kb on + strand, within MMP_RS04255 at 812.101 kb on + strand, within MMP_RS04255 at 812.124 kb on - strand, within MMP_RS04255 at 812.213 kb on - strand, within MMP_RS04255 at 812.242 kb on - strand, within MMP_RS04255 at 812.374 kb on - strand, within MMP_RS04255 at 812.457 kb on - strand at 812.457 kb on - strand at 812.458 kb on + strand at 812.466 kb on - strand at 812.468 kb on - strand at 812.468 kb on - strand at 812.487 kb on - strand at 812.488 kb on + strand at 812.506 kb on - strand at 812.507 kb on + strand at 812.527 kb on - strand at 812.528 kb on + strand at 812.632 kb on - strand, within MMP_RS04260 at 812.699 kb on + strand, within MMP_RS04260 at 812.913 kb on - strand, within MMP_RS04260 at 812.913 kb on - strand, within MMP_RS04260 at 813.010 kb on - strand, within MMP_RS04260 at 813.133 kb on - strand, within MMP_RS04260
Per-strain Table
Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 5mM L-Glutamate and 5mM L-Proline remove 810,639 - MMP_RS04245 0.87 +1.2 810,857 - +0.2 810,874 - +1.0 810,875 + -0.0 810,898 + +0.6 810,903 - +0.8 810,903 - -2.1 810,904 + +0.4 810,916 + +0.7 810,917 - -0.0 810,931 - +0.0 810,931 - +0.6 810,973 - +0.2 811,009 - -0.4 811,010 + +0.1 811,010 + -0.7 811,034 - -0.9 811,034 - +0.6 811,035 + +0.1 811,036 - +1.1 811,036 - +0.5 811,036 - -0.2 811,037 + -0.2 811,053 + +1.1 811,064 - +0.4 811,068 - +0.7 811,069 + -0.1 811,185 - -0.9 811,186 + -0.1 811,189 - +1.1 811,213 + -0.1 811,244 + -0.1 811,324 - MMP_RS04250 0.27 -0.5 811,368 + MMP_RS04250 0.46 +0.4 811,368 + MMP_RS04250 0.46 +0.2 811,400 - MMP_RS04250 0.61 +0.6 811,401 + MMP_RS04250 0.61 +0.6 811,441 + MMP_RS04250 0.79 -0.4 811,445 - MMP_RS04250 0.81 -0.5 811,453 - MMP_RS04250 0.85 +0.7 811,464 + -0.5 811,514 + -0.3 811,525 + +0.3 811,552 - +0.5 811,553 + -0.4 811,560 - +0.3 811,601 - -0.0 811,607 - -0.7 811,607 - -0.8 811,726 - MMP_RS04255 0.10 +0.3 811,872 - MMP_RS04255 0.27 -2.0 811,925 - MMP_RS04255 0.34 +0.1 811,925 - MMP_RS04255 0.34 -1.9 811,926 + MMP_RS04255 0.34 -0.7 811,935 - MMP_RS04255 0.35 -1.1 812,048 + MMP_RS04255 0.48 -0.6 812,101 + MMP_RS04255 0.54 +0.8 812,124 - MMP_RS04255 0.57 -3.0 812,213 - MMP_RS04255 0.68 -0.4 812,242 - MMP_RS04255 0.71 -0.0 812,374 - MMP_RS04255 0.87 -0.7 812,457 - -0.4 812,457 - +0.4 812,458 + +0.4 812,466 - +0.7 812,468 - -0.1 812,468 - -0.1 812,487 - +0.9 812,488 + +0.7 812,506 - -1.2 812,507 + -0.3 812,527 - +0.5 812,528 + +0.3 812,632 - MMP_RS04260 0.17 +0.5 812,699 + MMP_RS04260 0.26 -0.2 812,913 - MMP_RS04260 0.55 +0.0 812,913 - MMP_RS04260 0.55 +0.2 813,010 - MMP_RS04260 0.69 -0.6 813,133 - MMP_RS04260 0.86 -0.2
Or see this region's nucleotide sequence