Experiment: Mc formate, casamino free, with 5mM L-Glutamate and 5mM L-Proline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01430 and MMP_RS01435 are separated by 35 nucleotides MMP_RS01435 and MMP_RS01440 are separated by 39 nucleotides MMP_RS01440 and MMP_RS01445 are separated by 19 nucleotides MMP_RS01445 and MMP_RS01450 are separated by 13 nucleotides
MMP_RS01430: MMP_RS01430 - mechanosensitive ion channel family protein, at 264,845 to 265,900
_RS01430
MMP_RS01435: MMP_RS01435 - DMT family transporter, at 265,936 to 266,379
_RS01435
MMP_RS01440: MMP_RS01440 - NAAT family transporter, at 266,419 to 267,060
_RS01440
MMP_RS01445: MMP_RS01445 - hypothetical protein, at 267,080 to 267,796
_RS01445
MMP_RS01450: MMP_RS01450 - tRNA pseudouridine(38-40) synthase TruA, at 267,810 to 268,598
_RS01450
Position (kb) 266
267
268 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 265.495 kb on - strand, within MMP_RS01430 at 265.501 kb on - strand, within MMP_RS01430 at 265.502 kb on + strand, within MMP_RS01430 at 265.534 kb on - strand, within MMP_RS01430 at 265.535 kb on + strand, within MMP_RS01430 at 265.602 kb on - strand, within MMP_RS01430 at 265.603 kb on + strand, within MMP_RS01430 at 265.648 kb on + strand, within MMP_RS01430 at 265.881 kb on - strand at 265.881 kb on - strand at 265.882 kb on + strand at 265.883 kb on - strand at 265.884 kb on + strand at 265.884 kb on + strand at 266.003 kb on - strand, within MMP_RS01435 at 266.016 kb on + strand, within MMP_RS01435 at 266.141 kb on - strand, within MMP_RS01435 at 266.204 kb on - strand, within MMP_RS01435 at 266.325 kb on - strand, within MMP_RS01435 at 266.346 kb on + strand at 266.500 kb on - strand, within MMP_RS01440 at 266.932 kb on + strand, within MMP_RS01440 at 266.935 kb on - strand, within MMP_RS01440 at 266.935 kb on - strand, within MMP_RS01440 at 266.936 kb on + strand, within MMP_RS01440 at 266.971 kb on + strand, within MMP_RS01440 at 267.156 kb on - strand, within MMP_RS01445 at 267.161 kb on - strand, within MMP_RS01445 at 267.199 kb on - strand, within MMP_RS01445 at 267.307 kb on - strand, within MMP_RS01445 at 267.307 kb on - strand, within MMP_RS01445 at 267.441 kb on - strand, within MMP_RS01445 at 267.460 kb on - strand, within MMP_RS01445 at 267.506 kb on - strand, within MMP_RS01445 at 267.577 kb on + strand, within MMP_RS01445 at 267.702 kb on + strand, within MMP_RS01445 at 267.764 kb on - strand at 267.765 kb on + strand at 267.790 kb on + strand at 267.790 kb on + strand at 267.790 kb on + strand at 267.904 kb on - strand, within MMP_RS01450 at 267.904 kb on - strand, within MMP_RS01450 at 267.904 kb on - strand, within MMP_RS01450 at 267.905 kb on + strand, within MMP_RS01450 at 267.905 kb on + strand, within MMP_RS01450 at 267.926 kb on - strand, within MMP_RS01450 at 267.930 kb on + strand, within MMP_RS01450 at 267.988 kb on - strand, within MMP_RS01450 at 268.037 kb on - strand, within MMP_RS01450
Per-strain Table
Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 5mM L-Glutamate and 5mM L-Proline remove 265,495 - MMP_RS01430 0.62 +0.5 265,501 - MMP_RS01430 0.62 -0.2 265,502 + MMP_RS01430 0.62 +0.7 265,534 - MMP_RS01430 0.65 +0.4 265,535 + MMP_RS01430 0.65 +0.6 265,602 - MMP_RS01430 0.72 +0.0 265,603 + MMP_RS01430 0.72 +1.3 265,648 + MMP_RS01430 0.76 -1.7 265,881 - +0.8 265,881 - +0.0 265,882 + +0.6 265,883 - -1.6 265,884 + -1.1 265,884 + +0.0 266,003 - MMP_RS01435 0.15 -0.4 266,016 + MMP_RS01435 0.18 +0.4 266,141 - MMP_RS01435 0.46 +0.5 266,204 - MMP_RS01435 0.60 +0.2 266,325 - MMP_RS01435 0.88 +0.7 266,346 + +0.3 266,500 - MMP_RS01440 0.13 -2.9 266,932 + MMP_RS01440 0.80 -1.5 266,935 - MMP_RS01440 0.80 -0.8 266,935 - MMP_RS01440 0.80 -0.9 266,936 + MMP_RS01440 0.81 -1.4 266,971 + MMP_RS01440 0.86 -0.3 267,156 - MMP_RS01445 0.11 -1.1 267,161 - MMP_RS01445 0.11 -0.9 267,199 - MMP_RS01445 0.17 -1.4 267,307 - MMP_RS01445 0.32 -1.2 267,307 - MMP_RS01445 0.32 -2.6 267,441 - MMP_RS01445 0.50 -1.6 267,460 - MMP_RS01445 0.53 -1.4 267,506 - MMP_RS01445 0.59 -1.8 267,577 + MMP_RS01445 0.69 -1.4 267,702 + MMP_RS01445 0.87 -1.8 267,764 - -1.5 267,765 + -1.8 267,790 + -1.1 267,790 + -1.5 267,790 + -2.4 267,904 - MMP_RS01450 0.12 -0.9 267,904 - MMP_RS01450 0.12 -1.2 267,904 - MMP_RS01450 0.12 +0.4 267,905 + MMP_RS01450 0.12 -2.5 267,905 + MMP_RS01450 0.12 -1.1 267,926 - MMP_RS01450 0.15 -0.8 267,930 + MMP_RS01450 0.15 -0.4 267,988 - MMP_RS01450 0.23 -0.1 268,037 - MMP_RS01450 0.29 -0.7
Or see this region's nucleotide sequence