Experiment: Mc formate, casamino free, with 5mM L-Glutamate and 5mM L-Proline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00030 and MMP_RS00035 are separated by 243 nucleotides MMP_RS00035 and MMP_RS00040 are separated by 30 nucleotides MMP_RS00040 and MMP_RS00045 are separated by 36 nucleotides
MMP_RS00030: MMP_RS00030 - 16S ribosomal RNA, at 8,394 to 9,860
_RS00030
MMP_RS00035: MMP_RS00035 - DUF2098 family protein, at 10,104 to 10,385
_RS00035
MMP_RS00040: MMP_RS00040 - TIGR03576 family pyridoxal phosphate-dependent enzyme, at 10,416 to 11,480
_RS00040
MMP_RS00045: MMP_RS00045 - 2-oxoacid:acceptor oxidoreductase subunit alpha, at 11,517 to 12,608
_RS00045
Position (kb)
10
11
12 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 10.063 kb on + strand at 10.065 kb on + strand at 10.065 kb on + strand at 10.065 kb on + strand at 10.065 kb on + strand at 10.065 kb on + strand at 10.067 kb on + strand at 10.067 kb on + strand at 10.067 kb on + strand at 10.071 kb on - strand at 10.071 kb on - strand at 10.072 kb on + strand at 10.072 kb on + strand at 10.084 kb on + strand at 10.103 kb on - strand at 10.104 kb on + strand at 10.147 kb on + strand, within MMP_RS00035 at 10.147 kb on + strand, within MMP_RS00035 at 10.147 kb on + strand, within MMP_RS00035 at 10.150 kb on - strand, within MMP_RS00035 at 10.150 kb on - strand, within MMP_RS00035 at 10.151 kb on + strand, within MMP_RS00035 at 10.151 kb on + strand, within MMP_RS00035 at 10.155 kb on - strand, within MMP_RS00035 at 10.157 kb on - strand, within MMP_RS00035 at 10.158 kb on + strand, within MMP_RS00035 at 10.158 kb on + strand, within MMP_RS00035 at 10.158 kb on + strand, within MMP_RS00035 at 10.160 kb on - strand, within MMP_RS00035 at 10.237 kb on - strand, within MMP_RS00035 at 10.238 kb on + strand, within MMP_RS00035 at 10.238 kb on - strand, within MMP_RS00035 at 10.239 kb on - strand, within MMP_RS00035 at 10.239 kb on - strand, within MMP_RS00035 at 10.239 kb on - strand, within MMP_RS00035 at 10.239 kb on - strand, within MMP_RS00035 at 10.240 kb on + strand, within MMP_RS00035 at 10.240 kb on + strand, within MMP_RS00035 at 10.240 kb on + strand, within MMP_RS00035 at 10.240 kb on + strand, within MMP_RS00035 at 10.240 kb on + strand, within MMP_RS00035 at 10.240 kb on + strand, within MMP_RS00035 at 10.310 kb on + strand, within MMP_RS00035 at 10.326 kb on + strand, within MMP_RS00035 at 10.383 kb on - strand at 10.569 kb on - strand, within MMP_RS00040 at 10.569 kb on - strand, within MMP_RS00040 at 10.569 kb on - strand, within MMP_RS00040 at 10.604 kb on - strand, within MMP_RS00040 at 10.605 kb on + strand, within MMP_RS00040 at 10.625 kb on + strand, within MMP_RS00040 at 10.681 kb on - strand, within MMP_RS00040 at 10.731 kb on - strand, within MMP_RS00040 at 11.105 kb on + strand, within MMP_RS00040 at 11.197 kb on - strand, within MMP_RS00040 at 11.286 kb on + strand, within MMP_RS00040 at 11.315 kb on + strand, within MMP_RS00040 at 11.330 kb on + strand, within MMP_RS00040 at 11.354 kb on + strand, within MMP_RS00040 at 11.450 kb on + strand at 11.452 kb on - strand at 11.464 kb on - strand at 11.523 kb on - strand at 11.533 kb on + strand at 11.671 kb on + strand at 11.714 kb on + strand at 11.825 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 5mM L-Glutamate and 5mM L-Proline remove 10,063 + -0.4 10,065 + -0.1 10,065 + -0.1 10,065 + -1.9 10,065 + +0.3 10,065 + -0.1 10,067 + +0.3 10,067 + +0.1 10,067 + -0.1 10,071 - -1.7 10,071 - -0.9 10,072 + +0.2 10,072 + -1.5 10,084 + -1.0 10,103 - -0.9 10,104 + -0.5 10,147 + MMP_RS00035 0.15 +0.3 10,147 + MMP_RS00035 0.15 +0.4 10,147 + MMP_RS00035 0.15 -0.3 10,150 - MMP_RS00035 0.16 -0.9 10,150 - MMP_RS00035 0.16 +0.4 10,151 + MMP_RS00035 0.17 +1.4 10,151 + MMP_RS00035 0.17 -0.5 10,155 - MMP_RS00035 0.18 -2.6 10,157 - MMP_RS00035 0.19 +0.0 10,158 + MMP_RS00035 0.19 -0.4 10,158 + MMP_RS00035 0.19 -0.4 10,158 + MMP_RS00035 0.19 +0.2 10,160 - MMP_RS00035 0.20 -2.4 10,237 - MMP_RS00035 0.47 -0.6 10,238 + MMP_RS00035 0.48 -0.6 10,238 - MMP_RS00035 0.48 -0.6 10,239 - MMP_RS00035 0.48 -0.3 10,239 - MMP_RS00035 0.48 -1.2 10,239 - MMP_RS00035 0.48 -2.3 10,239 - MMP_RS00035 0.48 -0.7 10,240 + MMP_RS00035 0.48 +0.5 10,240 + MMP_RS00035 0.48 -0.6 10,240 + MMP_RS00035 0.48 -1.0 10,240 + MMP_RS00035 0.48 -0.3 10,240 + MMP_RS00035 0.48 -0.8 10,240 + MMP_RS00035 0.48 -0.0 10,310 + MMP_RS00035 0.73 +0.7 10,326 + MMP_RS00035 0.79 +0.2 10,383 - -2.4 10,569 - MMP_RS00040 0.14 -1.3 10,569 - MMP_RS00040 0.14 -3.0 10,569 - MMP_RS00040 0.14 -2.5 10,604 - MMP_RS00040 0.18 -2.8 10,605 + MMP_RS00040 0.18 -1.6 10,625 + MMP_RS00040 0.20 -3.1 10,681 - MMP_RS00040 0.25 -2.9 10,731 - MMP_RS00040 0.30 -2.4 11,105 + MMP_RS00040 0.65 -4.1 11,197 - MMP_RS00040 0.73 -2.2 11,286 + MMP_RS00040 0.82 +0.2 11,315 + MMP_RS00040 0.84 -1.2 11,330 + MMP_RS00040 0.86 -2.3 11,354 + MMP_RS00040 0.88 -2.3 11,450 + -2.5 11,452 - -3.0 11,464 - -1.8 11,523 - +0.2 11,533 + -0.1 11,671 + -1.9 11,714 + -2.5 11,825 + -0.7
Or see this region's nucleotide sequence