Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3211 and HP15_3212 overlap by 19 nucleotides HP15_3212 and HP15_3213 are separated by 282 nucleotides HP15_3213 and HP15_3214 are separated by 148 nucleotides HP15_3214 and HP15_3215 are separated by 119 nucleotides
HP15_3211: HP15_3211 - 5-formyltetrahydrofolate cyclo-ligase, at 3,361,999 to 3,362,682
_3211
HP15_3212: HP15_3212 - secreted protein, at 3,362,664 to 3,362,825
_3212
HP15_3213: HP15_3213 - threonine dehydratase biosynthetic, at 3,363,108 to 3,364,643
_3213
HP15_3214: HP15_3214 - ribose-5-phosphate isomerase A, at 3,364,792 to 3,365,475
_3214
HP15_3215: HP15_3215 - acyl-CoA dehydrogenase domain protein, at 3,365,595 to 3,366,761
_3215
Position (kb)
3363
3364
3365 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3362.272 kb on + strand, within HP15_3211 at 3362.280 kb on - strand, within HP15_3211 at 3362.297 kb on - strand, within HP15_3211 at 3362.297 kb on - strand, within HP15_3211 at 3362.487 kb on + strand, within HP15_3211 at 3362.630 kb on + strand at 3362.683 kb on + strand, within HP15_3212 at 3362.732 kb on - strand, within HP15_3212 at 3362.815 kb on - strand at 3362.828 kb on + strand at 3363.002 kb on - strand at 3363.100 kb on - strand at 3363.121 kb on + strand at 3363.129 kb on - strand at 3363.129 kb on - strand at 3363.218 kb on - strand at 3363.218 kb on - strand at 3363.226 kb on - strand at 3363.255 kb on + strand at 3363.263 kb on - strand, within HP15_3213 at 3363.288 kb on + strand, within HP15_3213 at 3363.296 kb on - strand, within HP15_3213 at 3363.735 kb on - strand, within HP15_3213 at 3363.800 kb on - strand, within HP15_3213 at 3363.961 kb on + strand, within HP15_3213 at 3364.069 kb on + strand, within HP15_3213 at 3364.117 kb on - strand, within HP15_3213 at 3364.218 kb on - strand, within HP15_3213 at 3364.308 kb on + strand, within HP15_3213 at 3364.316 kb on - strand, within HP15_3213 at 3364.336 kb on + strand, within HP15_3213 at 3364.393 kb on + strand, within HP15_3213 at 3364.436 kb on - strand, within HP15_3213 at 3364.436 kb on - strand, within HP15_3213 at 3364.461 kb on - strand, within HP15_3213 at 3364.549 kb on + strand at 3364.572 kb on + strand at 3364.572 kb on + strand at 3364.672 kb on + strand at 3364.747 kb on - strand at 3365.612 kb on + strand at 3365.616 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 3,362,272 + HP15_3211 0.40 -0.8 3,362,280 - HP15_3211 0.41 -1.2 3,362,297 - HP15_3211 0.44 +0.8 3,362,297 - HP15_3211 0.44 -1.1 3,362,487 + HP15_3211 0.71 -0.9 3,362,630 + -1.4 3,362,683 + HP15_3212 0.12 -0.1 3,362,732 - HP15_3212 0.42 -0.7 3,362,815 - -0.9 3,362,828 + -0.7 3,363,002 - -0.4 3,363,100 - -1.2 3,363,121 + -1.5 3,363,129 - -0.8 3,363,129 - +0.5 3,363,218 - -0.8 3,363,218 - -0.8 3,363,226 - -0.2 3,363,255 + +0.1 3,363,263 - HP15_3213 0.10 -1.8 3,363,288 + HP15_3213 0.12 -1.7 3,363,296 - HP15_3213 0.12 -0.4 3,363,735 - HP15_3213 0.41 -1.8 3,363,800 - HP15_3213 0.45 -1.2 3,363,961 + HP15_3213 0.56 -1.5 3,364,069 + HP15_3213 0.63 -3.2 3,364,117 - HP15_3213 0.66 -1.8 3,364,218 - HP15_3213 0.72 -0.5 3,364,308 + HP15_3213 0.78 -1.8 3,364,316 - HP15_3213 0.79 -2.2 3,364,336 + HP15_3213 0.80 -0.8 3,364,393 + HP15_3213 0.84 -1.0 3,364,436 - HP15_3213 0.86 -0.7 3,364,436 - HP15_3213 0.86 -0.1 3,364,461 - HP15_3213 0.88 +0.0 3,364,549 + -1.0 3,364,572 + -1.7 3,364,572 + +0.4 3,364,672 + -1.4 3,364,747 - +0.1 3,365,612 + -0.5 3,365,616 - -0.2
Or see this region's nucleotide sequence