Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS26465

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS26450 and BWI76_RS26455 overlap by 4 nucleotidesBWI76_RS26455 and BWI76_RS26460 overlap by 1 nucleotidesBWI76_RS26460 and BWI76_RS26465 overlap by 4 nucleotidesBWI76_RS26465 and BWI76_RS26470 overlap by 1 nucleotidesBWI76_RS26470 and BWI76_RS26475 overlap by 4 nucleotides BWI76_RS26450: BWI76_RS26450 - hypothetical protein, at 5,474,310 to 5,475,359 _RS26450 BWI76_RS26455: BWI76_RS26455 - 4-amino-4-deoxy-L-arabinose-phospho-UDP flippase, at 5,475,356 to 5,475,739 _RS26455 BWI76_RS26460: BWI76_RS26460 - 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE, at 5,475,739 to 5,476,077 _RS26460 BWI76_RS26465: BWI76_RS26465 - 4-amino-4-deoxy-L-arabinose lipid A transferase, at 5,476,074 to 5,477,729 _RS26465 BWI76_RS26470: BWI76_RS26470 - 4-deoxy-4-formamido-L-arabinose- phosphoundecaprenol deformylase, at 5,477,729 to 5,478,631 _RS26470 BWI76_RS26475: BWI76_RS26475 - bifunctional UDP-glucuronic acid oxidase/UDP-4-amino-4-deoxy-L-arabinose formyltransferase, at 5,478,628 to 5,480,613 _RS26475 Position (kb) 5476 5477 5478Strain fitness (log2 ratio) -1 0 1 2 3at 5475.163 kb on + strand, within BWI76_RS26450at 5475.178 kb on + strand, within BWI76_RS26450at 5475.186 kb on - strand, within BWI76_RS26450at 5475.482 kb on - strand, within BWI76_RS26455at 5475.482 kb on - strand, within BWI76_RS26455at 5475.719 kb on - strandat 5475.867 kb on + strand, within BWI76_RS26460at 5475.872 kb on + strand, within BWI76_RS26460at 5475.875 kb on - strand, within BWI76_RS26460at 5475.880 kb on - strand, within BWI76_RS26460at 5475.967 kb on + strand, within BWI76_RS26460at 5476.026 kb on + strand, within BWI76_RS26460at 5476.036 kb on + strand, within BWI76_RS26460at 5476.044 kb on - strandat 5476.211 kb on + strandat 5476.264 kb on + strand, within BWI76_RS26465at 5476.272 kb on - strand, within BWI76_RS26465at 5476.740 kb on - strand, within BWI76_RS26465at 5476.771 kb on - strand, within BWI76_RS26465at 5476.792 kb on + strand, within BWI76_RS26465at 5476.794 kb on - strand, within BWI76_RS26465at 5476.843 kb on + strand, within BWI76_RS26465at 5476.843 kb on + strand, within BWI76_RS26465at 5477.022 kb on - strand, within BWI76_RS26465at 5477.153 kb on + strand, within BWI76_RS26465at 5477.166 kb on - strand, within BWI76_RS26465at 5477.226 kb on + strand, within BWI76_RS26465at 5477.453 kb on + strand, within BWI76_RS26465at 5477.508 kb on + strand, within BWI76_RS26465at 5477.639 kb on - strandat 5477.842 kb on + strand, within BWI76_RS26470at 5477.890 kb on - strand, within BWI76_RS26470at 5477.890 kb on - strand, within BWI76_RS26470at 5477.940 kb on + strand, within BWI76_RS26470at 5477.948 kb on - strand, within BWI76_RS26470at 5478.061 kb on - strand, within BWI76_RS26470at 5478.102 kb on - strand, within BWI76_RS26470at 5478.282 kb on + strand, within BWI76_RS26470at 5478.498 kb on + strand, within BWI76_RS26470at 5478.649 kb on + strandat 5478.673 kb on + strandat 5478.684 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM
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5,475,163 + BWI76_RS26450 0.81 +0.0
5,475,178 + BWI76_RS26450 0.83 +0.5
5,475,186 - BWI76_RS26450 0.83 +0.1
5,475,482 - BWI76_RS26455 0.33 +1.7
5,475,482 - BWI76_RS26455 0.33 +1.4
5,475,719 - +1.4
5,475,867 + BWI76_RS26460 0.38 +0.5
5,475,872 + BWI76_RS26460 0.39 +1.7
5,475,875 - BWI76_RS26460 0.40 +1.6
5,475,880 - BWI76_RS26460 0.42 +1.4
5,475,967 + BWI76_RS26460 0.67 +1.5
5,476,026 + BWI76_RS26460 0.85 +1.8
5,476,036 + BWI76_RS26460 0.88 +1.7
5,476,044 - +1.5
5,476,211 + +2.5
5,476,264 + BWI76_RS26465 0.11 +2.2
5,476,272 - BWI76_RS26465 0.12 +1.9
5,476,740 - BWI76_RS26465 0.40 +2.2
5,476,771 - BWI76_RS26465 0.42 +2.2
5,476,792 + BWI76_RS26465 0.43 +2.6
5,476,794 - BWI76_RS26465 0.43 +2.1
5,476,843 + BWI76_RS26465 0.46 +2.2
5,476,843 + BWI76_RS26465 0.46 +1.8
5,477,022 - BWI76_RS26465 0.57 +2.1
5,477,153 + BWI76_RS26465 0.65 +1.6
5,477,166 - BWI76_RS26465 0.66 +2.1
5,477,226 + BWI76_RS26465 0.70 +1.9
5,477,453 + BWI76_RS26465 0.83 +2.1
5,477,508 + BWI76_RS26465 0.87 +2.0
5,477,639 - +2.2
5,477,842 + BWI76_RS26470 0.13 +2.1
5,477,890 - BWI76_RS26470 0.18 +2.4
5,477,890 - BWI76_RS26470 0.18 +1.7
5,477,940 + BWI76_RS26470 0.23 +2.2
5,477,948 - BWI76_RS26470 0.24 +3.0
5,478,061 - BWI76_RS26470 0.37 +2.3
5,478,102 - BWI76_RS26470 0.41 +2.3
5,478,282 + BWI76_RS26470 0.61 +1.4
5,478,498 + BWI76_RS26470 0.85 +2.1
5,478,649 + +1.7
5,478,673 + +2.0
5,478,684 + +1.4

Or see this region's nucleotide sequence