Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_863 and Pf6N2E2_864 overlap by 4 nucleotides Pf6N2E2_864 and Pf6N2E2_865 are separated by 3 nucleotides Pf6N2E2_865 and Pf6N2E2_866 are separated by 22 nucleotides
Pf6N2E2_863 - Transcriptional regulator, IclR family, at 1,011,922 to 1,012,728
_863
Pf6N2E2_864 - 2-keto-4-pentenoate hydratase (EC 4.2.1.80), at 1,012,725 to 1,013,525
_864
Pf6N2E2_865 - Fumarylacetoacetate hydrolase family protein, at 1,013,529 to 1,014,422
_865
Pf6N2E2_866 - hypothetical protein, at 1,014,445 to 1,015,140
_866
Position (kb)
1012
1013
1014 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1011.738 kb on + strand at 1011.738 kb on + strand at 1011.829 kb on - strand at 1011.829 kb on - strand at 1011.829 kb on - strand at 1011.829 kb on - strand at 1011.831 kb on + strand at 1011.832 kb on - strand at 1011.832 kb on - strand at 1011.832 kb on - strand at 1011.907 kb on + strand at 1011.907 kb on + strand at 1011.907 kb on + strand at 1011.907 kb on + strand at 1011.908 kb on - strand at 1011.908 kb on - strand at 1011.908 kb on - strand at 1011.908 kb on - strand at 1012.151 kb on + strand, within Pf6N2E2_863 at 1012.151 kb on + strand, within Pf6N2E2_863 at 1012.151 kb on + strand, within Pf6N2E2_863 at 1012.151 kb on + strand, within Pf6N2E2_863 at 1012.151 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.297 kb on + strand, within Pf6N2E2_863 at 1012.504 kb on + strand, within Pf6N2E2_863 at 1012.636 kb on + strand, within Pf6N2E2_863 at 1012.636 kb on + strand, within Pf6N2E2_863 at 1012.724 kb on + strand at 1012.724 kb on + strand at 1012.725 kb on - strand at 1012.892 kb on - strand, within Pf6N2E2_864 at 1012.892 kb on - strand, within Pf6N2E2_864 at 1012.892 kb on - strand, within Pf6N2E2_864 at 1012.965 kb on + strand, within Pf6N2E2_864 at 1012.965 kb on + strand, within Pf6N2E2_864 at 1012.966 kb on - strand, within Pf6N2E2_864 at 1012.982 kb on - strand, within Pf6N2E2_864 at 1012.982 kb on - strand, within Pf6N2E2_864 at 1012.982 kb on - strand, within Pf6N2E2_864 at 1013.085 kb on + strand, within Pf6N2E2_864 at 1013.086 kb on - strand, within Pf6N2E2_864 at 1013.155 kb on - strand, within Pf6N2E2_864 at 1013.156 kb on + strand, within Pf6N2E2_864 at 1013.156 kb on + strand, within Pf6N2E2_864 at 1013.157 kb on - strand, within Pf6N2E2_864 at 1013.157 kb on - strand, within Pf6N2E2_864 at 1013.157 kb on - strand, within Pf6N2E2_864 at 1013.157 kb on - strand, within Pf6N2E2_864 at 1013.204 kb on - strand, within Pf6N2E2_864 at 1013.222 kb on - strand, within Pf6N2E2_864 at 1013.379 kb on - strand, within Pf6N2E2_864 at 1013.427 kb on + strand, within Pf6N2E2_864 at 1013.427 kb on + strand, within Pf6N2E2_864 at 1013.427 kb on + strand, within Pf6N2E2_864 at 1013.428 kb on - strand, within Pf6N2E2_864 at 1013.537 kb on - strand at 1013.537 kb on - strand at 1013.767 kb on - strand, within Pf6N2E2_865 at 1014.195 kb on - strand, within Pf6N2E2_865 at 1014.204 kb on + strand, within Pf6N2E2_865 at 1014.366 kb on + strand at 1014.366 kb on + strand at 1014.367 kb on - strand at 1014.367 kb on - strand at 1014.444 kb on + strand at 1014.468 kb on - strand at 1014.468 kb on - strand at 1014.514 kb on + strand at 1014.514 kb on + strand at 1014.515 kb on - strand, within Pf6N2E2_866 at 1014.515 kb on - strand, within Pf6N2E2_866 at 1014.515 kb on - strand, within Pf6N2E2_866 at 1014.515 kb on - strand, within Pf6N2E2_866
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 1,011,738 + +0.4 1,011,738 + -0.5 1,011,829 - -0.7 1,011,829 - +0.8 1,011,829 - +0.0 1,011,829 - -1.0 1,011,831 + +1.0 1,011,832 - -0.6 1,011,832 - -0.0 1,011,832 - +1.7 1,011,907 + +1.1 1,011,907 + -1.5 1,011,907 + -1.4 1,011,907 + +0.9 1,011,908 - -1.0 1,011,908 - -1.0 1,011,908 - -0.0 1,011,908 - -0.6 1,012,151 + Pf6N2E2_863 0.28 -1.0 1,012,151 + Pf6N2E2_863 0.28 +0.1 1,012,151 + Pf6N2E2_863 0.28 -0.5 1,012,151 + Pf6N2E2_863 0.28 -1.0 1,012,151 + Pf6N2E2_863 0.28 -0.4 1,012,293 + Pf6N2E2_863 0.46 -0.8 1,012,293 + Pf6N2E2_863 0.46 -0.5 1,012,293 + Pf6N2E2_863 0.46 +1.9 1,012,293 + Pf6N2E2_863 0.46 -0.1 1,012,293 + Pf6N2E2_863 0.46 +0.8 1,012,293 + Pf6N2E2_863 0.46 -0.1 1,012,293 + Pf6N2E2_863 0.46 +0.3 1,012,293 + Pf6N2E2_863 0.46 -0.2 1,012,293 + Pf6N2E2_863 0.46 +0.7 1,012,293 + Pf6N2E2_863 0.46 -1.3 1,012,293 + Pf6N2E2_863 0.46 +0.0 1,012,293 + Pf6N2E2_863 0.46 +0.1 1,012,297 + Pf6N2E2_863 0.46 -0.0 1,012,504 + Pf6N2E2_863 0.72 +0.9 1,012,636 + Pf6N2E2_863 0.88 -0.0 1,012,636 + Pf6N2E2_863 0.88 -1.6 1,012,724 + +0.6 1,012,724 + -0.3 1,012,725 - +0.9 1,012,892 - Pf6N2E2_864 0.21 -1.5 1,012,892 - Pf6N2E2_864 0.21 -0.7 1,012,892 - Pf6N2E2_864 0.21 +0.6 1,012,965 + Pf6N2E2_864 0.30 +1.8 1,012,965 + Pf6N2E2_864 0.30 -0.8 1,012,966 - Pf6N2E2_864 0.30 -2.4 1,012,982 - Pf6N2E2_864 0.32 +0.4 1,012,982 - Pf6N2E2_864 0.32 -0.6 1,012,982 - Pf6N2E2_864 0.32 +0.2 1,013,085 + Pf6N2E2_864 0.45 -0.2 1,013,086 - Pf6N2E2_864 0.45 -0.5 1,013,155 - Pf6N2E2_864 0.54 -0.8 1,013,156 + Pf6N2E2_864 0.54 +0.2 1,013,156 + Pf6N2E2_864 0.54 +1.3 1,013,157 - Pf6N2E2_864 0.54 +0.3 1,013,157 - Pf6N2E2_864 0.54 -0.7 1,013,157 - Pf6N2E2_864 0.54 -0.1 1,013,157 - Pf6N2E2_864 0.54 -0.8 1,013,204 - Pf6N2E2_864 0.60 -0.2 1,013,222 - Pf6N2E2_864 0.62 -0.7 1,013,379 - Pf6N2E2_864 0.82 +0.6 1,013,427 + Pf6N2E2_864 0.88 +0.9 1,013,427 + Pf6N2E2_864 0.88 +0.9 1,013,427 + Pf6N2E2_864 0.88 -0.2 1,013,428 - Pf6N2E2_864 0.88 +0.2 1,013,537 - -0.2 1,013,537 - +0.5 1,013,767 - Pf6N2E2_865 0.27 +0.5 1,014,195 - Pf6N2E2_865 0.74 +1.2 1,014,204 + Pf6N2E2_865 0.76 -0.4 1,014,366 + +0.7 1,014,366 + -0.3 1,014,367 - +0.8 1,014,367 - -0.2 1,014,444 + +0.2 1,014,468 - -0.1 1,014,468 - +0.8 1,014,514 + -1.1 1,014,514 + -0.5 1,014,515 - Pf6N2E2_866 0.10 -0.1 1,014,515 - Pf6N2E2_866 0.10 +0.2 1,014,515 - Pf6N2E2_866 0.10 +0.6 1,014,515 - Pf6N2E2_866 0.10 +0.8
Or see this region's nucleotide sequence