Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_666 and Pf6N2E2_667 overlap by 1 nucleotides Pf6N2E2_667 and Pf6N2E2_668 are separated by 55 nucleotides
Pf6N2E2_666 - Acetoin dehydrogenase E1 component beta-subunit (EC 1.2.4.-), at 771,042 to 772,058
_666
Pf6N2E2_667 - Dihydrolipoamide acetyltransferase component (E2) of acetoin dehydrogenase complex (EC 2.3.1.-), at 772,058 to 773,170
_667
Pf6N2E2_668 - 2,3-butanediol dehydrogenase, R-alcohol forming, (R)- and (S)-acetoin-specific (EC 1.1.1.4), at 773,226 to 774,320
_668
Position (kb) 772
773
774 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 771.378 kb on + strand, within Pf6N2E2_666 at 771.379 kb on - strand, within Pf6N2E2_666 at 771.379 kb on - strand, within Pf6N2E2_666 at 771.506 kb on - strand, within Pf6N2E2_666 at 771.506 kb on - strand, within Pf6N2E2_666 at 771.526 kb on - strand, within Pf6N2E2_666 at 771.601 kb on - strand, within Pf6N2E2_666 at 771.633 kb on + strand, within Pf6N2E2_666 at 771.633 kb on + strand, within Pf6N2E2_666 at 771.776 kb on - strand, within Pf6N2E2_666 at 771.884 kb on + strand, within Pf6N2E2_666 at 771.993 kb on + strand at 771.993 kb on + strand at 771.993 kb on + strand at 771.993 kb on + strand at 771.993 kb on + strand at 771.994 kb on - strand at 771.994 kb on - strand at 771.994 kb on - strand at 771.994 kb on - strand at 771.994 kb on - strand at 772.072 kb on + strand at 772.782 kb on - strand, within Pf6N2E2_667 at 772.895 kb on + strand, within Pf6N2E2_667 at 772.895 kb on + strand, within Pf6N2E2_667 at 772.896 kb on - strand, within Pf6N2E2_667 at 772.896 kb on - strand, within Pf6N2E2_667 at 772.948 kb on - strand, within Pf6N2E2_667 at 772.989 kb on + strand, within Pf6N2E2_667 at 772.990 kb on - strand, within Pf6N2E2_667 at 773.388 kb on + strand, within Pf6N2E2_668 at 773.388 kb on + strand, within Pf6N2E2_668 at 773.389 kb on - strand, within Pf6N2E2_668 at 773.568 kb on + strand, within Pf6N2E2_668 at 773.568 kb on + strand, within Pf6N2E2_668 at 773.569 kb on - strand, within Pf6N2E2_668 at 773.569 kb on - strand, within Pf6N2E2_668 at 773.572 kb on - strand, within Pf6N2E2_668 at 773.572 kb on - strand, within Pf6N2E2_668 at 773.572 kb on - strand, within Pf6N2E2_668 at 773.572 kb on - strand, within Pf6N2E2_668 at 773.590 kb on - strand, within Pf6N2E2_668 at 773.676 kb on + strand, within Pf6N2E2_668 at 773.676 kb on + strand, within Pf6N2E2_668 at 774.147 kb on + strand, within Pf6N2E2_668 at 774.148 kb on - strand, within Pf6N2E2_668 at 774.148 kb on - strand, within Pf6N2E2_668 at 774.148 kb on - strand, within Pf6N2E2_668
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 771,378 + Pf6N2E2_666 0.33 -1.8 771,379 - Pf6N2E2_666 0.33 +0.6 771,379 - Pf6N2E2_666 0.33 -0.4 771,506 - Pf6N2E2_666 0.46 -0.6 771,506 - Pf6N2E2_666 0.46 -0.2 771,526 - Pf6N2E2_666 0.48 +1.1 771,601 - Pf6N2E2_666 0.55 +1.4 771,633 + Pf6N2E2_666 0.58 +1.0 771,633 + Pf6N2E2_666 0.58 -0.9 771,776 - Pf6N2E2_666 0.72 -0.0 771,884 + Pf6N2E2_666 0.83 +2.0 771,993 + -1.1 771,993 + +1.2 771,993 + +0.8 771,993 + +2.1 771,993 + -0.5 771,994 - +0.8 771,994 - +2.4 771,994 - +0.5 771,994 - +0.0 771,994 - -0.2 772,072 + +0.9 772,782 - Pf6N2E2_667 0.65 +0.6 772,895 + Pf6N2E2_667 0.75 +0.5 772,895 + Pf6N2E2_667 0.75 -0.5 772,896 - Pf6N2E2_667 0.75 +0.8 772,896 - Pf6N2E2_667 0.75 +0.2 772,948 - Pf6N2E2_667 0.80 -0.4 772,989 + Pf6N2E2_667 0.84 +0.5 772,990 - Pf6N2E2_667 0.84 +0.0 773,388 + Pf6N2E2_668 0.15 +1.6 773,388 + Pf6N2E2_668 0.15 +0.1 773,389 - Pf6N2E2_668 0.15 +0.4 773,568 + Pf6N2E2_668 0.31 +0.2 773,568 + Pf6N2E2_668 0.31 +2.6 773,569 - Pf6N2E2_668 0.31 +0.8 773,569 - Pf6N2E2_668 0.31 -1.2 773,572 - Pf6N2E2_668 0.32 -0.3 773,572 - Pf6N2E2_668 0.32 -0.8 773,572 - Pf6N2E2_668 0.32 +0.4 773,572 - Pf6N2E2_668 0.32 +0.2 773,590 - Pf6N2E2_668 0.33 -0.4 773,676 + Pf6N2E2_668 0.41 -0.5 773,676 + Pf6N2E2_668 0.41 +0.3 774,147 + Pf6N2E2_668 0.84 +0.2 774,148 - Pf6N2E2_668 0.84 -0.0 774,148 - Pf6N2E2_668 0.84 +1.0 774,148 - Pf6N2E2_668 0.84 +2.8
Or see this region's nucleotide sequence